Infant formula milk powder for promoting brain development as well as preparation method and application thereof
A technology of infant formula and milk powder, which is applied in the fields of application, dairy products, whey, etc., can solve the problems of low content and insufficient ability to supply sialic acid, and achieve the effect of increasing content, excellent effect, and promoting the development of brain nerve tissue
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Embodiment 1-6
[0036] Embodiment 1-6 A kind of preparation method of infant formula milk powder that promotes brain development
[0037]Embodiments 1-6 respectively prepare a kind of infant formula milk powder for promoting brain development, comprising the following steps carried out in sequence:
[0038] (1) Preparation of composite components
[0039] Prepare the composite components in Examples 1-6 in advance respectively according to the composite component batching table shown in Table 1, and mix the corresponding composite component batches evenly to obtain the corresponding composite components;
[0040] Table 1 embodiment 1-6 composite component batching table
[0041]
[0042]
[0043]
[0044] In Table 1, proportional relationship 1 is the proportional relationship between soybean oil, rapeseed oil, sunflower oil and walnut oil in the compound vegetable oil;
[0045] Proportional relationship 2 is the proportional relationship between calcium carbonate, calcium chloride...
Embodiment 7
[0056] Example 7 Fatty acid content of infant formula for promoting brain development
[0057] Using the assay method in GB 5009.168-2016 "Determination of Fatty Acids in Food", the infant formula milk powder (marked as MX1-MX6 group), breast milk (marked as MX7 group) and traditional The content of two essential fatty acids, linoleic acid and α-linolenic acid, in formula milk powder (marked as MX8 group), the specific results are as follows:
[0058] Table 4 fatty acid detection result table
[0059]
[0060] The results show that compared with the traditional formula milk powder, the fatty acid content and ratio in the brain-promoting infant formula milk powder of the present invention are closer to breast milk, and are easier to be absorbed and utilized by infants, so as to achieve fatty acid supplementation, promote central nervous development, and benefit brain development Effect.
Embodiment 8
[0061] Example 8 The synergistic effect of sialyllactose, ganglioside, compound vegetable powder, curcumin and probiotic powder on the proliferation of cerebral cortex neural stem cells
[0062] Isolate the cerebral cortex tissue of the fetal mouse under sterile conditions, make a single cell suspension, centrifuge to remove the supernatant, add DMEM / F12 complete medium containing 2% B27 for culture; the fetal mouse cerebral cortex nerves in good growth state Stem cells were randomly divided into five groups and seeded in five 96-well plates. The complete medium used in each group was as follows:
[0063] Control group: without adding any substances (that is, without adding 3'-sialyllactose, 6'-sialyllactose, gangliosides, compound vegetable powder, curcumin and Bifidobacterium animalis milk subsp. i797 powder);
[0064] Group Y1: simultaneously add 3'-sialyllactose, 6'-sialyllactose, ganglioside, compound vegetable powder, curcumin and Bifidobacterium animalis milk subsp.
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