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A kind of phosphatidic acid molecularly imprinted fluorescent nanoparticle and its preparation method

A fluorescent nanometer and molecular imprinting technology, applied in the field of analytical chemistry, can solve the problems of lack of specificity of molecularly imprinted polymers, residues of template molecules, and low utilization of templates, so as to improve selectivity and specificity, high specificity, and improve efficiency effect

Active Publication Date: 2022-03-08
NANJING NORMAL UNIVERSITY
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Problems solved by technology

[0005] Technical problems to be solved: Aiming at the technical problems of low template utilization rate and difficulty in elution in current molecular imprinting technology, and lack of specificity of phosphatidic acid molecularly imprinted polymers in the prior art, the present invention provides a phosphatidic acid molecularly imprinted fluorescent nanoparticle and Its preparation method can solve the problem that template molecules will remain in molecularly imprinted polymers in traditional methods, and significantly improve the specificity of molecularly imprinted fluorescent nanoparticles; in addition, the present invention can adopt static Separation of products and by-products in a single way avoids the use of centrifuges and makes the operation easier

Method used

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  • A kind of phosphatidic acid molecularly imprinted fluorescent nanoparticle and its preparation method
  • A kind of phosphatidic acid molecularly imprinted fluorescent nanoparticle and its preparation method
  • A kind of phosphatidic acid molecularly imprinted fluorescent nanoparticle and its preparation method

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Embodiment 1

[0036] Preparation of aldehyde-modified silica nanoparticles (SN-CHO), see figure 1 ,Specific steps are as follows:

[0037] Step 1: First, ammonia solution (25 wt.%, 30.4 mL), ethanol (200 mL), and water (306 mL) were mixed in a 1 L round bottom flask and stirred at 600 rpm; subsequently, ortho-silicon was added A mixture of ethyl acetate (45.6 mL) and ethanol (456 mL) was stirred at room temperature for 8 hours; finally, silica nanoparticles were collected by centrifugation (10,000 rpm, 10 min) and washed with ethanol at least three times at room temperature After drying under vacuum for 24 h, silica nanoparticles (SN) were obtained with a yield of 37%.

[0038] Step 2: Disperse SN (14 g) into anhydrous toluene (280 mL) by magnetic stirring in a 500 mL three-necked round bottom flask; then, add 3-aminopropyltriethoxysilane ( APTES, 2.37 g, 10.7 mmol), and refluxed at an oil bath temperature of 130 °C for 12 h; after the reaction was completed, the mixture was cooled to ro...

Embodiment 2

[0041] Preparation of fingolimod phosphatidic acid bis(tetrabutylammonium) salt functionalized silica nanoparticles (named SN-FP(2TBA)), see figure 2 , the specific preparation process is as follows:

[0042] The template molecule fingolimod phosphatidic acid (FP) (29 mg, 0.075 mol) was added to a dispersion of SN-CHO (0.25 g) in 2 mL of phosphate (10 mM, pH 7.4) under magnetic stirring at room temperature After 24 h of reaction, the nanoparticles were collected by centrifugation (10,000 rpm, 10 min), washed three times with phosphate buffer, pure water, and methanol, respectively, and then the nanoparticles were vacuum-dried at room temperature to obtain FP-modified silica nanoparticles. (SN-FP). Then 0.25 g SN-FP was dispersed in methanol (2 mL), tetrabutylammonium hydroxide (TBA-OH, 0.3 mmol) was added, and the reaction was stirred at room temperature for 12 h, then washed with methanol three times, and vacuum dried at room temperature to obtain the template The molecula...

Embodiment 3

[0044] Fingolimod phosphatidic acid molecularly imprinted fluorescent nanoparticles (poly(IMID-EGDMA)-MIP) and their control polymers (poly(IMID-EGDMA)-NIP) were prepared.

[0045] Preparation of fingolimod phosphatidic acid molecularly imprinted fluorescent nanoparticles (poly(IMID-EGDMA)-MIP), such as image 3 As shown, the specific preparation process is as follows:

[0046] (1) SN-FP(2TBA) (50 mg), fluorescent functional monomer 1,8-anthracene-bis(3-vinyl-1H-imidazole) dibromide (IMID) (5.7 mg, 10.3 μmol), The cross-linker ethylene glycol bis(methacrylate) (EGDMA) (65 μL, 344 μmol) and the initiator azobisisoheptanenitrile (ABDV) (5 mg, 20.1 μmol) were dissolved in 10 mL of chloroform and mixed with 0.2 mL of methanol in liquid.

[0047] The synthetic method of the fluorescent functional monomer 1,8-anthracene-bis(3-vinyl-1H-imidazole) dibromide (IMID) is as follows: First, 1,8-dibromoanthracene (1.09 g, 2.0 mmol) It was dissolved in 25 mL of acetonitrile, then N-vinyli...

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Abstract

A phosphatidic acid molecularly imprinted fluorescent nanoparticle and a preparation method thereof belong to the field of analytical chemistry. The preparation method comprises the following steps: first, phosphatidic acid is covalently bonded to the surface of the carrier through a Schiff base reaction; then the phosphatidic acid-modified carrier is dispersed into a solvent with a fluorescent functional monomer, a cross-linking agent and an initiator. Heating under stirring or shaking conditions initiates the polymerization reaction; secondly, the upper solution is removed by standing still, and the carrier material is washed with chloroform solvent; finally, the molecularly imprinted fluorescent nanoparticles are eluted from the carrier surface with chloroform solution by ultrasound to obtain phosphatidic acid molecules Imprint fluorescent nanoparticles. The present invention adopts the strategy of immobilizing templates with covalent bonds to prepare phosphatidic acid molecularly imprinted fluorescent nanoparticles, which is not only simple to prepare and convenient to operate, but also can avoid template molecules remaining in molecularly imprinted polymers, and can significantly improve the specificity of molecularly imprinted fluorescent nanoparticles. sexual recognition.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a phosphatidic acid molecularly imprinted fluorescent nanoparticle and a preparation method thereof. Background technique [0002] Molecularly Imprinted Polymer (MIP) is a kind of high molecular polymer with special molecular recognition ability. The complex is formed by covalent action, and then a cross-linking agent and an initiator are added to obtain a molecularly imprinted prepolymerization solution; then, a polymerization reaction is initiated to obtain a high molecular polymer containing template molecules; finally, the template molecules in the polymer are removed, so that the An imprinted cavity that is complementary to the shape of the template molecule is left in the polymer, and the functional group of the functional monomer remains in the cavity, so the imprinted cavity has the ability to selectively recognize the template molecule. Due to the simple preparation,...

Claims

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Application Information

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IPC IPC(8): C08F226/06C08F222/14C08F2/44C08J9/26G01N21/64B01J20/281B01J20/28B01J20/30C08L39/04
Inventor 李前进伯杰·塞勒格伦王婷婷金雨王奋英李建林
Owner NANJING NORMAL UNIVERSITY
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