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Coniferous tree rhizosphere biocontrol growth-promoting bacterium bidirectional Burkholderia ambifaria strain and application thereof

A technology that promotes growth of bacteria and needles, applied in the field of microorganisms, can solve problems such as poor growth of seedlings, lag in mining and utilization of disease-resistant bacteria resources, etc.

Active Publication Date: 2021-04-16
HEILONGJIANG PROV FOREST PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As far as the research on Burkholderia is concerned, the excavation and utilization of coniferous, especially northern conifer such as sylvestris sylvestris, Korean pine and other rhizosphere growth-promoting and disease-resistant bacterial resources are seriously lagging behind. In recent years, there has been excessive chemical pesticides in the northern conifer nursery. The problem of poor growth of seedlings caused by reasons such as use and continuous cropping obstacles, the introduction of rhizosphere probiotics is an effective way to promote the growth of seedlings and improve the micro-ecological environment of the rhizosphere, so screen and excavate the rhizosphere growth-promoting and stress-resistant bacterial resources of northern coniferous trees , which has important scientific significance for improving the stress resistance level of coniferous seedlings, ensuring the production of strong seedlings and improving the survival rate of subsequent afforestation

Method used

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  • Coniferous tree rhizosphere biocontrol growth-promoting bacterium bidirectional Burkholderia ambifaria strain and application thereof
  • Coniferous tree rhizosphere biocontrol growth-promoting bacterium bidirectional Burkholderia ambifaria strain and application thereof
  • Coniferous tree rhizosphere biocontrol growth-promoting bacterium bidirectional Burkholderia ambifaria strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Isolation and Identification of Strain ZB-155

[0054] The bacterial strain ZB-155 isolated from the rhizosphere soil of Pinus sylvestris sylvestris plantation in Zhanggutai Experimental Forest Farm, Liaoning Province was identified, and the specific methods were as follows:

[0055] 1) Physiological and biochemical measurements of the bacterial strain ZB-155 were performed referring to the "Berger Bacteria Identification Handbook" and "Common Bacterial System Identification Handbook", and the colony morphology and characteristics were described.

[0056] Strain ZB-155 can form 1-2mm colonies after being cultured on NA medium for 24 hours. There is no protrusion in the middle of the colony, milky white, smooth and shiny surface, neat edges, opaque, and no fluorescence ( figure 1 -A, figure 1 -B). Bacteria are short rod-shaped, Gram staining is negative ( figure 2 ).

[0057] Strain ZB-155 can grow anaerobically, phenylalanine dehydrogenase, amylase, urease, V-P tes...

Embodiment 2

[0064] Determination of Antibacterial Spectrum Activity of Rhizosphere Bacteria ZB-155 Forest Pathogenic Bacteria

[0065] Plate antagonism: Draw a cross on the back of the PDA plate through the center point, ferment and culture the rhizosphere bacteria ZB-155 in NA medium for 24 hours, respectively absorb 50 μl of the bacterial liquid and spot on the cross line 3.0cm away from the center point 4 At one point, after 24°C culture at 25°C, inoculate the test pathogenic fungus in the center of the PDA medium, take the place where the bacteria solution was not inoculated as the control treatment, put it in the incubator for 25°C culture, and measure when the control plate is covered with the plate Treat the colony diameter and calculate the bacteriostatic rate.

[0066]

[0067] Antibacterial experiment of fermented liquid: after the rhizosphere bacterium ZB-155 was shaken and cultivated in NA medium for 72 hours, the fermented liquid was transferred into a 50ml sterile centrif...

Embodiment 3

[0073] Determination of IAA Production and Phosphorus Solubilizing Activity of Rhizosphere Bacteria ZB-155

[0074] Qualitative detection of IAA: Inoculate ZB-155 into R2A medium containing 200 mg / L L-tryptophan, culture in a constant temperature shaking incubator at 28°C and 180rpm for 48 hours, then use a sterile pipette to draw 2ml of bacterial liquid into the sterile Add an equal volume of Sackowchi's chromogen to the bacterial centrifuge tube, mix quickly and fully, and place in the dark for 30 minutes at room temperature, and observe the color change.

[0075] Quantitative detection of IAA: Accurately weigh 10mg of IAA, first dissolve it with a small amount of absolute ethanol, then dilute to 100ml with distilled water (concentration 100μg / ml), and then dilute to 0, 4, 8, 12, 16, 20, 24μg / ml respectively ml, take 1ml of different concentrations, add 4ml of Sackowchi's chromogenic reagent respectively, and let it stand in the dark for 30min at room temperature. 535nm The...

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Abstract

The invention provides a coniferous tree rhizosphere biocontrol growth-promoting bacterium, namely a bidirectional Burkholderia ambifaria strain and application thereof, belonging to the technical field of microorganisms. The coniferous tree rhizosphere biocontrol growth-promoting bacterium, namely the bidirectional Burkholderia ambifaria strain ZB-155 has the capabilities of producing IAA and dissolving phosphorus, and has a remarkable promoting effect on the growth of coniferous trees such as pinus sylvestris; moreover, the strain also has the capacity of secreting cellulase and protease; and the strain and fermentation products thereof have an antibacterial effect on pathogenic bacteria such as seedling damping-off, poplar rotten bark disease, pine wilt, hazelnut leaf blight and hazelnut brown spot, and can also improve the nutritional ingredients of plant rhizosphere soil, so the invention provides application of the strain and derivative products thereof in plant cultivation and soil improvement.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bidirectional Burkholderia strain of conifer rhizosphere biocontrol and growth-promoting bacteria and an application thereof. Background technique [0002] The rhizosphere microbiome plays an irreplaceable role in promoting plant growth, improving mineral absorption, biological disease control, and improving plant stress resistance, so it is called the "plant second genome". Rhizosphere growth-promoting bacteria (PGPR, plant growth promoting rhizobacteria) are typical representatives of probiotics, mainly through biological nitrogen fixation, secretion of hormones, production of iron chelating carriers, phosphorus solubilization, etc. to improve plant rhizosphere nutrient absorption capacity and promote growth. Representative PGPR genera mainly include Pseudomonas, Bacillus, Flavobacterium, Sinorhizobium, Burkholderia, etc. . The mechanism of rhizosphere growth-p...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P21/00C12R1/01
Inventor 邓勋宋小双遇文婧刘欣周琦马晓乾王琪李俊刘艳红姜瑞凤梁新宇王笑松
Owner HEILONGJIANG PROV FOREST PROTECTION INST
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