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Liquid culture medium for paecilomyces lilacinus and preparation method of paecilomyces lilacinus microbial inoculum

A technology of Paecilomyces lilacinus and Paecilomyces lilacinus is applied in the directions of methods using spores, methods based on microorganisms, biochemical equipment and methods, etc., which can solve the problems of waste of resources, environment, pollution, etc., and achieve a clear process, The effect of increasing oxygen supply and short production cycle

Active Publication Date: 2021-04-20
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it is not processed and utilized in time, it will also cause waste of resources and environmental pollution.

Method used

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  • Liquid culture medium for paecilomyces lilacinus and preparation method of paecilomyces lilacinus microbial inoculum
  • Liquid culture medium for paecilomyces lilacinus and preparation method of paecilomyces lilacinus microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The first step: the preparation of the liquid medium of Paecilomyces lilacinus;

[0021] Put 2g of fungus chaff, 0.10g of zinc chloride, 0.20g of sodium nitrate, and 12ml of distilled water into a 50ml Erlenmeyer flask, mix well with high-pressure steam at 121°C, and sterilize for 30 minutes.

[0022] The second step: Paecilomyces lilacinus plate preparation;

[0023] The Paecilomyces lilacinus block was placed in a plate culture dish filled with sterilized PDA medium by aseptic operation, placed in an incubator at 29° C. for 10 days and then harvested.

[0024] Step 3: By washing the plates with Paecilomyces lilacinus, a concentration of 10 7 spores / ml of Paecilomyces lilacinus inoculum.

[0025] Step 4: Insert the bacterium chaff medium in Example 1, add 0.14ml of inoculum into the sterilized 50ml Erlenmeyer flask containing the bacterium chaff medium, seal the bottle mouth with a parafilm, and place it at 29°C Harvest after culturing in a shaker for 6 days.

[00...

Embodiment 2

[0029] The difference between this embodiment and embodiment 1 is only: the volume of the distilled water added in the first step is 14ml. Example 2 Prepare Paecilomyces lilacinus liquid culture medium and spore production amount by using various bacterium chaffs in Table 1

Embodiment 3

[0031] The difference between this embodiment and embodiment 1 is only: the quality of the sodium nitrate added in the first step is 0.16g. Example 3 Prepare Paecilomyces lilacinus liquid culture medium and spore production amount by using various fungus chaffs in Table 1

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Abstract

The invention provides a liquid culture medium for paecilomyces lilacinus and a preparation method of a paecilomyces lilacinus microbial inoculum. The paecilomyces lilacinus microbial inoculum is obtained by uniformly mixing mushroom bran, water, zinc chloride and sodium nitrate and then performing high-pressure steam sterilization, wherein 6-8ml of water, 0.04-0.06 g of zinc chloride and 0.08-0.12 g of sodium nitrate are added into each gram of mushroom bran; and inoculating the paecilomyces lilacinus inoculant into the liquid culture medium, and culturing at 27-31 DEG C for 4-6 days to obtain the paecilomyces lilacinus microbial inoculum. The raw materials are agricultural wastes and are economical and easy to obtain, and the culture method is simple to operate, low in production cost and high in sporulation quantity.

Description

technical field [0001] The invention belongs to the technical field of fermentation and cultivation, and in particular relates to a liquid medium for Paecilomyces lilacinus and a method for preparing a Paecilomyces lilacinus agent. Background technique [0002] Paecilomyces lilacinus is an inhabitant fungus widely distributed in soil and plant roots, and is considered to be the most promising biological agent for the control of plant nematode diseases. It has the characteristics of high efficiency, spectrum, long-term effect, safety, no pollution, no residue and so on. Paecilomyces lilacinus can parasitize the eggs, larvae and adults of plant nematodes. During the infection process, through the joint action of mycelium mechanical pressure and secreted extracellular enzymes, the nematodes and worms burst and die, and can produce Substances with nematicidal activity inhibit the hatching of nematode eggs and strongly inhibit the killing of 2nd instar larvae to control crop nem...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/79
Inventor 于晓斌程志强高峰赵春莉刘文丛杨桂霞
Owner JILIN AGRICULTURAL UNIV