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Surface hydrophilic modification method of polystyrene micro-fluidic chip

A microfluidic chip, polystyrene technology, applied in the field of analysis and detection, can solve problems such as wide application limitation, separation failure, harsh reaction conditions, etc., achieving simple components and preparation processes, suppressing the generation of bubbles, mild and environmentally friendly conditions Effect

Inactive Publication Date: 2021-04-27
NINGBO POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the PS microfluidic chip also has some defects: Like other polymer materials, its surface is highly hydrophobic, and most biological macromolecules such as proteins are strongly adsorbed on the surface of its microchannels due to hydrophobic interactions and other reasons, resulting in poor analysis results. Distortion, poor separation efficiency, and even separation failure when the adsorption is severe; secondly, the hydrophobic surface makes it difficult to fill the micron-sized channel with aqueous solution, and the surface is very easy to generate air bubbles, which makes the flow path disconnected
At present, the surface modification technologies for polymer microfluidic chips mainly include dynamic coating method and static induction grafting modification. The former needs to add the substance to be coated in the buffer solution every time it is injected. It may affect the properties of the sample or buffer; although the latter can permanently change the surface properties of the chip, the reaction conditions are harsh and require anhydrous, oxygen-free, plasma or ultraviolet light source irradiation, etc., the operation steps are complicated and the cost is high. limit its wide application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for surface hydrophilic modification of a polystyrene microfluidic chip, comprising the following steps:

[0025] S1, preparation of hydrophilic treatment agent A, followed by dopamine 2g / L, 2-(diethanolamino)ethanesulfonate sodium 0.5g / L, lauryl amidopropyl sulfobetaine 0.1g / L, inositol 0.1g / L, Triton X-1000.1g / L, and sodium azide 0.1g / L were added to the tris(hydroxymethyl)aminomethane buffer solution 50mmol / L, fully stirred and mixed, and then the solvent water was added, and continued to stir and mix uniform;

[0026] S2, the obtained hydrophilic treatment agent A is filtered by a microporous membrane, and the obtained filtrate is hydrophilic coating one;

[0027] S3, preparing hydrophilic treatment agent B, successively add polyethylene glycol diamine 1g / L, polyethylene glycol disuccinic acid 1g / L, tromethamine sulfate lauryl sulfate 0.5g / L, inositol 0.1g / L L. Add Triton X-1000.1g / L and sodium azide 0.1g / L into tris(hydroxymethyl)aminomethane buffer sol...

Embodiment 2

[0032] S1, prepare hydrophilic treatment agent A, successively add dopamine 3g / L, 2-(diethanolamino)ethanesulfonate sodium 0.8g / L, lauramide propyl hydroxy sulfobetaine 0.3g / L, inositol 0.2g / L, Triton X-1000.2g / L, and sodium azide 0.1g / L were added to tris(hydroxymethyl)aminomethane buffer solution 90mmol / L, fully stirred and mixed, then added solvent water, and continued to stir and mix uniform;

[0033] S2, the obtained hydrophilic treatment agent A is filtered by a microporous membrane, and the obtained filtrate is hydrophilic coating one;

[0034] S3, preparing hydrophilic treatment agent B, successively add polyethylene glycol diamine 2g / L, polyethylene glycol disuccinic acid 2g / L, tromethamine sulfate lauryl sulfate 0.8g / L, inositol 0.2g / L L, Triton X-1000.2g / L, sodium azide 0.1g / L are added to tris(hydroxymethyl)aminomethane buffer solution 90mmol / L, fully stirred and mixed, then solvent water is added, continue to stir and mix ;

[0035] S4, the obtained hydrophili...

Embodiment 3

[0039] A method for surface hydrophilic modification of a polystyrene microfluidic chip, comprising the following steps:

[0040] S1, prepare hydrophilic treatment agent A, successively add dopamine 3.5g / L, 2-(diethanolamino) ethanesulfonate sodium 1.25g / L, lauramide propyl hydroxy sulfobetaine 0.55g / L, inositol 0.3 g / L, Triton X-1000.3g / L, and sodium azide 0.1g / L are added to tris(hydroxymethyl)aminomethane buffer solution 125mmol / L, fully stirred and mixed, then solvent water is added, and the stirring is continued mix;

[0041] S2, the obtained hydrophilic treatment agent A is filtered by a microporous membrane, and the obtained filtrate is hydrophilic coating one;

[0042] S3, preparing hydrophilic treatment agent B, successively add polyethylene glycol diamine 3g / L, polyethylene glycol disuccinic acid 3g / L, tromethamine sulfate lauryl sulfate 1.25g / L, inositol 0.3g / L L, Triton X-1000.3g / L, sodium azide 0.1g / L are added to tris(hydroxymethyl)aminomethane buffer solution 12...

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PUM

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Abstract

The invention provides a surface hydrophilic modification method of a polystyrene micro-fluidic chip, which comprises the following steps: preparing a hydrophilic treatment agent A, and filtering the obtained hydrophilic treatment agent A by using a microfiltration membrane to obtain a filtrate which is a hydrophilic coating I; preparing a hydrophilic treatment agent B, and filtering the obtained hydrophilic treatment agent B by using a microfiltration membrane to obtain a filtrate which is a hydrophilic coating II; sucking 1-2 [mu]L of the hydrophilic coating I by using a pipette, slowly adding the hydrophilic coating I to the top end of a chip flow channel to be subjected to hydrophilic treatment, and after the hydrophilic coating I automatically fills the whole flow channel, placing the chip in a drying oven for drying for 1-2 h; and sucking 1-2 [mu]L of the hydrophilic coating II by using a pipette, slowly adding the hydrophilic coating II to the top end of the chip flow channel treated by using the hydrophilic coating I, and after the hydrophilic coating II automatically fills the whole flow channel, putting the chip into a drying box, and conducting drying for 4-5h. The method is high in modification efficiency, long in hydrophilicity retention time after modification, good in stability and free of damage to the chip.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a surface hydrophilic modification method of a polystyrene microfluidic chip. Background technique [0002] Microfluidic chip technology is an epoch-making analytical science and technology based on multidisciplinary foundations such as microelectronics technology, biological and chemical technology, and detection and analysis technology. As a new technology produced by interdisciplinary integration, it is considered to be one of the most important inventions that will affect the future of mankind. As an important development frontier of current analytical science, it represents the development direction of future analytical instruments towards miniaturization and integration. [0003] At present, the common materials used in the processing and production of microfluidic chips mainly include monocrystalline silicon wafers, glass and thermoplastic polymers. Monocrystalline si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L2300/161
Inventor 沃燕波刘兴艳施美霞申芳嫡邬智喜
Owner NINGBO POLYTECHNIC