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Breeding method of high-temperature-resistant chlorella for aquaculture

A technology for aquaculture and chlorella, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problems of increasing production costs, unfavorable for high density of chlorella, waste of energy, etc., to achieve Improve the success rate, protect the stability, promote the effect of growth separation

Pending Publication Date: 2021-04-30
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is not conducive to high-density, factory farming of chlorella
If air conditioning is used to cool down, energy is greatly wasted and production costs are increased.

Method used

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  • Breeding method of high-temperature-resistant chlorella for aquaculture
  • Breeding method of high-temperature-resistant chlorella for aquaculture
  • Breeding method of high-temperature-resistant chlorella for aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Breeding high temperature resistant chlorella

[0031] 1. Collect microalgae liquid from outdoor aquaculture water, and separate relatively pure chlorella single algae colony (purity is more than 80%, calculated by counting method) through plate marking and coating method.

[0032] Plate medium configuration is as follows:

[0033] (1) Nutrient solution mother solution formula (1L)

[0034] Table 1

[0035] Material name molecular formula level Weight (g) sodium nitrate NaNO 3

AR 75 Potassium dihydrogen phosphate dihydrate NaH 2 PO 4 2H 2 o

AR 5 Dipotassium hydrogen phosphate anhydrous K 2 HPO 4

AR 4 sodium bicarbonate NaHCO 3

AR 1 ammonium chloride NH 4 Cl

AR 1 yeast extract BR 2 plant peptone BR 1

[0036] Vitamin Mother Liquid Formula (100mL):

[0037] Table 2

[0038] Material name molecular formula level Weight (mg) ...

Embodiment 2

[0057] Embodiment 2: the contrast of breeding method

[0058] Culture medium of the present invention: culture medium in embodiment 1;

[0059] Conventional microalgae medium: BG11 (sodium nitrate 1.5g, dipotassium hydrogen phosphate 0.04g, magnesium sulfate heptahydrate 0.075g, calcium chloride heptahydrate 0.036g, sodium carbonate 0.02g, citric acid 0.006g, ferric ammonium citrate 0.006 g, EDTA0.001g, sterile water 1L.)

[0060] On the basis of the method in Example 1, different culture media and whether to add cell protection solution were used for comparison;

[0061] Breeding method of the present invention: embodiment 1 method;

[0062] Comparative breeding method: on the basis of the method in Example 1, replace the culture medium of the present invention with conventional microalgae culture medium and do not add cell protection solution, cultivate under the conditions of light intensity 10000lux and culture temperature 45°C for breeding;

[0063] Each method adopts ...

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PUM

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Abstract

The invention relates to the field of aquaculture, and discloses a breeding method of high-temperature-resistant chlorella for aquaculture. According to the method, high-temperature-resistant algae species suitable for a high-temperature environment are screened out by continuously screening algae species in a high-temperature adaptability mode while a self-developed cell protection liquid and an optimized breeding culture medium are used in the screening process to carry out high-temperature protection and proliferation promotion on microalgae cells, so that the integrity of microalgae cell walls at the high temperature can be effectively protected, the chloroplast stability is protected, the breeding success rate is increased, the screened chlorella can adapt to the high temperature of 45 DEG C to the maximum extent, and certain technical support is provided for chlorella production in southern China.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a method for breeding high-temperature resistant chlorella for aquaculture. Background technique [0002] Chlorella is an ancient and healthy single-celled plant that is widely distributed in the ocean and land. It is rich in active substances such as protein, amino acid, fatty acid, and vitamin, making it widely used in the fields of pharmaceutical industry, food industry, and biological bait. development prospects. In the pharmaceutical industry, microalgae can be added to make blood lipid-lowering drugs, blood pressure lowering drugs, anti-tumor drugs and other internal medicines, and can also be made into external medicines for treating burns, scalds and sports injuries. In terms of biological bait, microalgae itself is a pure natural green biological bait, which is easily absorbed and digested by fish and shrimp. Using microalgae biological bait to replace traditional bait, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/12C12N1/02
Inventor 黄成潭潘军叶蕾黄敏
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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