CHI3L1/LN detection card as well as preparation method and application thereof
A CHI3L1 and detection card technology, applied in the field of nanomaterials and nanomedicine, can solve the problems of unsatisfactory rapid detection, inaccurate quantification, low sensitivity, etc., and achieve the effect of fast and simple detection, strong fluorescent signal and high sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0070] The present invention also provides a preparation method of CHI3L1 / LN detection card, comprising the following steps:
[0071] Step 1: preparing activated rare earth nanoprobes according to the preparation method of claim 3;
[0072] Step 2: Prepare rare earth nanoprobe-labeled LN antibody 1, CHI3L1 antibody 1, and rabbit IgG antibody: add LN antibody 1, CHI3L1 antibody 1, and rabbit IgG antibody to the activated rare earth nanoprobe in step 1 at 50-200 μg / 200 μl, After blocking with blocking solution, centrifuge at high speed, wash and resuspend with containing preservative solution, and store at 4°C in the dark;
[0073] Step 3: Prepare coating membrane: respectively use CHI3L1 antibody 2, LN antibody 2 and goat anti-rabbit IgG antibody as the detection line T1, T2 line and quality control line in parallel on the nitrocellulose membrane for coating and drying;
[0074] Step 4: Prepare the bonding pad: Soak the bonding pad in 20mM Tris-HCl with pH 8.0 containing 0.5% ...
Embodiment 1
[0090] The rare earth nanoprobe of this embodiment is sodium yttrium fluoride coated with sodium erbium fluoride with a core-shell structure, and has a particle size of 20nm to 30nm. Its composition is:
[0091] NaErF 4 @NaYF 4 , where NaErF 4 It is an all-erbium-doped core structure; NaYF 4 is the shell, @ means NaYF 4 Coated in NaErF 4 surface.
[0092] Further, the rare earth nanoprobe is stable in the ground state, and emits three emission fluorescences in the wavelength ranges of 500-550nm, 640-680nm and 1500-1600nm under the action of an excitation light source of 808nm. NaErF 4 @NaYF 4 Fluorescence spectra of rare earth nanoprobes excited at 808nm, such as figure 2 shown. NaErF 4 @NaYF 4 TEM images of rare earth nanoprobes, such as image 3 shown.
[0093] The method for preparing the rare earth nanoprobe of this embodiment comprises the following steps:
[0094] Step 1: Synthesis of erbium sodium fluoride core structure: In the container, add oleic acid ...
Embodiment 2
[0098] like figure 1 As shown, the CHI3L1 / LN detection card of the present embodiment includes:
[0099] Substrate 1; as the substrate of the CHI3L1 / LN detection card;
[0100] Coating film 4; arranged in the middle of the upper surface of the substrate 1;
[0101] Bonding pad 3: overlapped at one end of the upper surface of the coating film 4
[0102] Sample pad 2; overlapped with one end of the upper surface of the binding pad 3;
[0103] Absorbent paper 4; overlapped on the other end of the upper surface of the coating film 3;
[0104] Wherein, the binding pad 3 is sprayed with microsphere threads 31, and the microsphere threads 31 are the rare earth nanoprobe-labeled LN antibody 1, CHI3L1 antibody 1, and rabbit IgG antibody provided in Example 1; A detection line T1 41, a detection line T2 42 and a quality control line 43 are arranged in sequence, the detection line 41 is close to the binding pad 3, the detection line T141, the detection line T2 42 and the quality cont...
PUM
Property | Measurement | Unit |
---|---|---|
Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com