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Broiler chicken antibiotic-replacement feed composite additive rich in enzyme and bacteria and preparation method thereof

A technology of compound additives and enzymes, applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of declining benefits, raising breeding costs, and increasing disease rates.

Pending Publication Date: 2021-05-07
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a compound feed additive for broiler chickens to replace antibiotics, so as to solve the problems of decreased production performance, increased disease rate, increased breeding costs and declining benefits in the breeding process of broilers after antibiotics are banned.

Method used

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  • Broiler chicken antibiotic-replacement feed composite additive rich in enzyme and bacteria and preparation method thereof
  • Broiler chicken antibiotic-replacement feed composite additive rich in enzyme and bacteria and preparation method thereof
  • Broiler chicken antibiotic-replacement feed composite additive rich in enzyme and bacteria and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 feeding probiotics

[0019] (1) Activation of bacteria

[0020] The probiotics used in the examples of the present invention were purchased from the Microorganism Collection Center, Bacillus subtilis CICC 24675 and Clostridium butyricum CICC 23847.

[0021] The Bacillus subtilis activation medium adopts LB, and the formula is: 0.5% of yeast extract, 1% of tryptone, 1% of sodium chloride, and 2% of agar in solid medium. Bacillus subtilis was inoculated on the plate medium, and cultured at 37° C. for 24 hours to obtain activated strains.

[0022] Clostridium butyricum activation medium adopts enhanced Clostridium medium, the formula is: beef extract 1%, glucose 05%, tryptone 1%, yeast extract powder 0.3%, sodium chloride 0.5%, soluble starch 0.1%, cysteine Amino acid hydrochloride 0.05%, sodium acetate 0.3%, solid medium plus agar 2%. Inoculate Clostridium butyricum on the plate medium and culture anaerobically at 37°C for 12 hours to ob...

Embodiment 2

[0029] The preparation of embodiment 2 feed enzyme preparations

[0030] The heterologous expression host bacteria of the enzyme preparations used in the examples of the present invention were all constructed in this laboratory.

[0031] (1) Preparation of seed solution

[0032] Pick the monoclonal Pichia host bacteria in the ultra-clean workbench and inoculate them in 200mLYEPD medium, with 1% yeast extract, 2% peptone, and 2% glucose, and culture at 30°C and 250rpm for 36-38h, until the wet cell weight reaches 30g / L.

[0033] Batch fermentation of the seeds was carried out in two stages using 50 liter and 500 liter fermenters, respectively. The seeds were inoculated in a 50L fermenter containing 30L sterile fermentation medium at 30°C, 350rpm, and 20% (w / v) ammonia solution was added to adjust the pH to 4.5 for fermentation until the wet cell weight reached OD600>30. In the second stage, the seeds were transferred to a 500-liter fermenter containing 350 liters of sterile...

Embodiment 3

[0039] The preparation of embodiment 3 oligomannan oligosaccharides

[0040] (1) Preparation of hydrolyzate of konjac oligosaccharide

[0041] Take 100kg of pure water, heat it indirectly to 60°C, add β-mannanase to make the enzyme activity in the enzymolysis tank reach 50IU / mL, stir and mix evenly. Weigh 12.5kg of konjac powder, slowly add it into the enzymatic hydrolysis tank, add while stirring, continue to add 12.5kg of konjac powder after 20 minutes; stir and hydrolyze for 1 hour after all the addition, measure the viscosity, reducing sugar and total sugar content. After the reaction, heat up to 80°C and keep it warm for 10 minutes to terminate the enzymatic hydrolysis reaction, cool to 50°C, filter and intercept the crude fiber in the reaction system with a sieve to obtain the crude filtrate of konjac oligosaccharides.

[0042] (2) Centrifugal filtration

[0043] The starch, coarse fiber particles and residues in the oligosaccharide solution were removed by 8000rpm hig...

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Abstract

The invention discloses a broiler chicken antibiotic-replacement feed composite additive rich in enzyme and bacteria and a preparation method thereof, and belongs to the field of feed additives. The enzyme and bacteria composite additive is composed of probiotics, a feed enzyme preparation and prebiotics. The composite additive in enzyme and bacteria is added into broiler chicken feed according to the ratio of 400g / t, and can effectively replace composite antibiotics for use, and broiler chicken antibiotic-free feed with the composite additive rich in enzyme and bacteria added can obtain good production performance and economic benefits after broiler chickens are fed.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to a composite feed additive rich in enzyme bacteria element for broilers and a preparation method thereof. Background technique [0002] With the continuous development of society, people's demand for animal protein continues to increase, which directly promotes the rapid development of the aquaculture industry. How to provide consumers with highly nutritious and food-safe meat, eggs, milk and other foods is an issue that the farming industry has been discussing. The ban on antibiotics is the best contribution to the food industry, and it will definitely be a step forward for the development of the industry. A solid step. The Ministry of Agriculture and Rural Affairs issued Announcement No. 194. From July 1, 2020, feed manufacturers will stop producing commercial feed containing growth-promoting drug feed additives (except Chinese herbal medicines). So far, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K10/18A23K20/163A23K20/189
CPCA23K50/75A23K10/18A23K20/163A23K20/189
Inventor 黄遵锡苗华彪唐湘华韩楠玉
Owner YUNNAN NORMAL UNIV
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