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Preparation method of cistanche enzymolysis oligosaccharide concentrated solution as well as obtained product and application

A Cistanche enzyme and technology of Cistanche, applied in the direction of cosmetic preparations, dressing preparations, skin care preparations, etc., can solve the problems of unpublished Cistanche oligosaccharide mixtures, research reports that have no efficacy of Cistanche oligosaccharide mixtures, etc., and achieve reduction Effects of skin cell damage, reduction of free radical formation, and inhibition of melanin formation

Pending Publication Date: 2021-05-11
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The prior art has not yet disclosed the method of preparing the Cistanche oligosaccharide mixture by enzymatic degradation of the polysaccharide extracted from Cistanche deserticola, and there is no research report on the efficacy of this Cistanche oligosaccharide mixture

Method used

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  • Preparation method of cistanche enzymolysis oligosaccharide concentrated solution as well as obtained product and application
  • Preparation method of cistanche enzymolysis oligosaccharide concentrated solution as well as obtained product and application
  • Preparation method of cistanche enzymolysis oligosaccharide concentrated solution as well as obtained product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The extraction of embodiment 1 cistanche bulb polysaccharide

[0051] Fresh Cistanche bulbs are taken, sliced, dried at 40°C until the water content is <4wt%, and then pulverized, passed through a 30-mesh sieve to collect Cistanche bulb powder. Weigh 1000g of cistanche bulb powder, add purified water to make up the volume according to the solid-to-liquid ratio of 1:5, and make a solution. At 40°C, 400W ultrasonic was used to treat the solution for 40min, and the lignified structure of Cistanche deserticola powder was preliminarily destroyed. Add 1 g of cellulase, 0.4 g of hemicellulase and 1 ml of laccase to the sonicated Cistanche powder solution, and treat at 45° C. for 40 min. The enzyme-treated solution was heated to 80°C for 1 hour, and the obtained water extract was filtered, and the filter residue was extracted once with purified water at a solid-to-liquid ratio of 1:5, and the obtained water extract was filtered. The two water extracts were combined, and 10L o...

Embodiment 2

[0052] The preparation of embodiment 2 cistanche enzymolysis oligosaccharide concentrate

[0053] Take 20 g of cistanche bulb polysaccharide prepared in Example 1, make a solution according to a solid-to-liquid ratio of 1:5, add different degrading enzymes to the solution, and perform enzymolysis at the appropriate temperature, pH and dosage of each degrading enzyme Treatment, the enzymolysis solution obtained after enzymolysis was inactivated at 70°C for 40min, then 1wt% activated carbon was added to the enzymolysis solution, decolorized at 55°C for 40min, the decolorized enzymolysis solution was centrifuged, and the supernatant was taken. The solution is then passed through a 0.22 micron membrane filter to remove insoluble impurities. Then, the enzymolysis solution was dialyzed overnight with a dialysis bag with a molecular weight cut-off of 5000 Da to further remove polysaccharides with too high molecular weight, and the permeate was collected to obtain an enzymatic oligosa...

Embodiment 3

[0061] Fresh Cistanche bulbs are taken, sliced, dried at 40°C until the water content is <4wt%, and then pulverized, passed through a 10-mesh sieve to collect Cistanche bulb powder. Weigh an appropriate amount of cistanche bulb powder, add purified water to constant volume, and prepare a 5L solution according to the set solid-to-liquid ratio. The solution was ultrasonically treated to initially destroy the lignified structure of Cistanche powder. After ultrasonic treatment, enzyme treatment can be carried out, and then the solution after ultrasonic or enzyme treatment is subjected to water extraction, the obtained water extract is filtered, and 93% industrial ethanol, which is 5 times the volume of the water extract, is slowly added to it under stirring. , standing and settling, the supernatant is brown-black, the precipitate is black, and the texture is fine. Pour off the supernatant, add 93% industrial ethanol to the precipitate and wash it three times to obtain a loose bro...

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Abstract

The invention relates to a preparation method of a cistanche enzymolysis oligosaccharide concentrated solution as well as an obtained product and application. The cistanche enzymolysis oligosaccharide concentrated solution is obtained by degrading cistanche bulb polysaccharide with degrading enzyme. The enzyme is one or more of glucoamylase, mannase, galactosidase and xylanase. The cistanche enzymolysis oligosaccharide concentrated solution is prepared by adopting an enzymolysis method, has mild conditions, no non-sugar by-products, high oligosaccharide yield, and no toxic and irritating by-products such as furfural, and is more suitable for application in cosmetics. The enzymolysis method has advantages of low requirements on reaction equipment, low equipment loss, low energy consumption, low production water consumption and higher economic and environmental benefits. The obtained oligosaccharide concentrated solution has functions of whitening, moisturizing, antioxidation, sunscreen, post-sun repair and the like when being applied to cosmetics, and can be applied to cosmetics as a moisturizing, whitening, sunscreen, post-sun repair and antioxidation component.

Description

technical field [0001] The present invention relates to a new product of Cistanche cistanche enzymatically hydrolyzed oligosaccharides prepared from the Chinese herbal medicine Cistanche cistanche as raw material, as well as the preparation method and application of the product, and further relates to a product with multiple functions of moisturizing, whitening, sun protection, post-sun repair and anti-oxidation, In particular, the preparation method of Cistanche deserticola oligosaccharide concentrate with whitening effect and its products and applications belong to the fields of natural medicinal chemistry and cosmetics. Background technique [0002] Skin is the largest organ in the human body, composed of epidermis, dermis and subcutaneous tissue, and contains appendages. As the body's first barrier to the external environment, the skin is constantly affected by various microbial, chemical and physical factors. Light, environmental pollution in modern society, and radiat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/00A61K8/73A61K8/60A61Q17/04A61Q19/00A61Q19/02A61Q19/08
CPCC12P19/14C12P19/00A61K8/73A61K8/60A61Q17/04A61Q19/00A61Q19/02A61Q19/004A61Q19/08
Inventor 王冠凤董艳美刘玉敏张晓鸥谢敬石艳丽栾贻宏郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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