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Carbon quantum dot fluorescent probe and method for detecting content of thiamphenicol

A technology of carbon quantum dots and fluorescent probes, which is applied in the field of food safety detection, can solve the problem of little work in detecting TAP, and achieve excellent selectivity and anti-interference ability, sensitive detection limit, and low cost.

Active Publication Date: 2021-05-28
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation of CDs from crayfish shell litter has not been reported, and there is little work on the detection of TAP using CDs probes

Method used

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  • Carbon quantum dot fluorescent probe and method for detecting content of thiamphenicol
  • Carbon quantum dot fluorescent probe and method for detecting content of thiamphenicol
  • Carbon quantum dot fluorescent probe and method for detecting content of thiamphenicol

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Embodiment 1

[0041] (1) Types and sources of raw materials

[0042] Crayfish shells were collected in the garbage of a restaurant in Changsha, China; reference drugs thiamphenicol (TAP), florfenicol (FF), chloramphenicol (CAP), amoxicillin (AMX), sulfonamides The drug (SDI), penicillin (PG), doxycycline hydrochloride (DOX), melamine (MEL) (98%), phenylindole (DAPI) and dimethyl sulfoxide (DMSO) are all produced by Shanghai Yuanye Biotechnology Ltd.; L-cysteine ​​was purchased from Chengdu Kelong Chemical Reagent Co., Ltd.; BR buffer was H 3 PO 4 -HAc-H 3 BO 3 Mixed acid system; all reagents are of analytical grade and used without further purification. The Hela cell line was obtained from Shanghai Binxin Biotechnology Co., Ltd. (www.bio-nano.com). Ultrapure water (18.2 MΩ·cm) was produced by a Milli-Q ultrapure water system (Millipore, Burlington, MA, USA, www.millipore.com). The milk and fish samples were all from Ya'an Jixuan Supermarket.

[0043] (2) Synthesis of carbon quantum d...

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Abstract

The invention provides a carbon quantum dot fluorescent probe and a method for detecting the content of thiamphenicol in food and living cells. The preparation method of the carbon quantum dot fluorescent probe comprises the following steps of (1) crushing the crayfish shells, (2) dispersing the crayfish shell powder in ultrapure water, adding L-cysteine, transferring the mixture into a reaction kettle, and carrying out carbonization treatment at 200 DEG C for 8 hours by adopting a hydrothermal method, and (3) after cooling to room temperature, centrifuging the obtained substance, filtering, collecting supernate, filtering with a 0.22 mum filter membrane, dialyzing and purifying, freeze-drying the obtained solution to obtain carbon quantum dots, and dissolving the carbon quantum dots in water to obtain the carbon quantum dot fluorescent probe. The carbon quantum dot fluorescent probe provided by the invention can be effectively quenched in a static quenching manner, is wide in linear detection range, reasonable in detection limit, high in sensitivity and good in selectivity, can be well applied to rapid detection of trace thiamphenicol residues in food and living cells, and is not interfered by a complex environment.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, in particular to a carbon quantum dot fluorescent probe and a method for detecting the content of thiamphenicol in food or living cells. Background technique [0002] Amino-alcohol antibiotics are broad-spectrum antibiotics that are widely used in the veterinary treatment of food-borne animals. Chloramphenicol (CAP) is one of the typical representatives of amide-alcohol antibiotics, but the 1994 European Community Regulation 1430 / 94 announcement clearly prohibited the use of chloramphenicol. Thiamphenicol (TAP) is similar to CAP in structure, but its potency is stronger than chloramphenicol, and it is the best substitute for CAP in treating poultry and animal diseases. Existing studies have shown that TAP has a wide range of effects on the treatment of bacterial infections of the gastrointestinal tract and respiratory tract, bacterial prostatitis and sexually transmitted diseases. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15B82Y40/00B82Y20/00G01N21/64
CPCC09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/6428G01N21/643G01N21/6486G01N21/6456G01N2021/6432C01P2002/01C01P2002/72C01P2002/85C01P2004/64
Inventor 陈姝娟欧阳莞琳刘梦茹邹立扣敖晓琳刘书亮何利申光辉杨勇
Owner SICHUAN AGRI UNIV
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