A kind of carbon quantum dot fluorescent probe and method for detecting thiamphenicol content
A technology of thiamphenicol and carbon quantum dots, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., can solve the problem of little work in detecting TAP, achieve excellent selectivity, anti-interference ability, and sensitivity Detection limit, effect of good biocompatibility
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[0041] (1) Types and sources of raw materials
[0042] Crayfish shells were collected in the garbage of a restaurant in Changsha, China; reference drugs thiamphenicol (TAP), florfenicol (FF), chloramphenicol (CAP), amoxicillin (AMX), sulfonamides The drug (SDI), penicillin (PG), doxycycline hydrochloride (DOX), melamine (MEL) (98%), phenylindole (DAPI) and dimethyl sulfoxide (DMSO) are all produced by Shanghai Yuanye Biotechnology Ltd.; L-cysteine was purchased from Chengdu Kelong Chemical Reagent Co., Ltd.; BR buffer was H 3 PO 4 -HAc-H 3 BO 3 Mixed acid system; all reagents are of analytical grade and used without further purification. The Hela cell line was obtained from Shanghai Binxin Biotechnology Co., Ltd. (www.bio-nano.com). Ultrapure water (18.2 MΩ·cm) was produced by a Milli-Q ultrapure water system (Millipore, Burlington, MA, USA, www.millipore.com). The milk and fish samples were all from Ya'an Jixuan Supermarket.
[0043] (2) Synthesis of carbon quantum d...
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