A kind of carbon quantum dot fluorescent probe and method for detecting thiamphenicol content

A technology of thiamphenicol and carbon quantum dots, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., can solve the problem of little work in detecting TAP, achieve excellent selectivity, anti-interference ability, and sensitivity Detection limit, effect of good biocompatibility

Active Publication Date: 2022-04-26
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the preparation of CDs from crayfish shell litter has not been reported, and there is little work on the detection of TAP using CDs probes

Method used

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  • A kind of carbon quantum dot fluorescent probe and method for detecting thiamphenicol content
  • A kind of carbon quantum dot fluorescent probe and method for detecting thiamphenicol content
  • A kind of carbon quantum dot fluorescent probe and method for detecting thiamphenicol content

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Embodiment 1

[0041] (1) Types and sources of raw materials

[0042] Crayfish shells were collected in the garbage of a restaurant in Changsha, China; reference drugs thiamphenicol (TAP), florfenicol (FF), chloramphenicol (CAP), amoxicillin (AMX), sulfonamides The drug (SDI), penicillin (PG), doxycycline hydrochloride (DOX), melamine (MEL) (98%), phenylindole (DAPI) and dimethyl sulfoxide (DMSO) are all produced by Shanghai Yuanye Biotechnology Ltd.; L-cysteine ​​was purchased from Chengdu Kelong Chemical Reagent Co., Ltd.; BR buffer was H 3 PO 4 -HAc-H 3 BO 3 Mixed acid system; all reagents are of analytical grade and used without further purification. The Hela cell line was obtained from Shanghai Binxin Biotechnology Co., Ltd. (www.bio-nano.com). Ultrapure water (18.2 MΩ·cm) was produced by a Milli-Q ultrapure water system (Millipore, Burlington, MA, USA, www.millipore.com). The milk and fish samples were all from Ya'an Jixuan Supermarket.

[0043] (2) Synthesis of carbon quantum d...

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Abstract

The invention provides a carbon quantum dot fluorescent probe and a method for detecting the content of thiamphenicol in food and living cells. The preparation method of the carbon quantum dot fluorescent probe is: (1) pulverizing the crayfish shell; (2) dispersing the crayfish shell powder in ultrapure water, adding L-cysteine, and transferring the mixture to the reactor (3) After cooling to room temperature, centrifuge the resultant, collect the supernatant by filtration, filter through a 0.22 μm filter membrane, and purify by dialysis. The obtained solution is freeze-dried to obtain Carbon quantum dots are dissolved in water to obtain carbon quantum dot fluorescent probes. The carbon quantum dot fluorescent probe provided by the present invention can be effectively quenched by static quenching, and has a wide linear detection range, reasonable detection limit, high sensitivity and good selectivity, and can be well applied to traces in food and living cells. Rapid detection of thiamphenicol residues without interference from complex environments.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, in particular to a carbon quantum dot fluorescent probe and a method for detecting the content of thiamphenicol in food or living cells. Background technique [0002] Amino-alcohol antibiotics are broad-spectrum antibiotics that are widely used in the veterinary treatment of food-borne animals. Chloramphenicol (CAP) is one of the typical representatives of amide-alcohol antibiotics, but the 1994 European Community Regulation 1430 / 94 announcement clearly prohibited the use of chloramphenicol. Thiamphenicol (TAP) is similar to CAP in structure, but its potency is stronger than chloramphenicol, and it is the best substitute for CAP in treating poultry and animal diseases. Existing studies have shown that TAP has a wide range of effects on the treatment of bacterial infections of the gastrointestinal tract and respiratory tract, bacterial prostatitis and sexually transmitted diseases. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65C01B32/15B82Y40/00B82Y20/00G01N21/64
CPCC09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/6428G01N21/643G01N21/6486G01N21/6456G01N2021/6432C01P2002/01C01P2002/72C01P2002/85C01P2004/64
Inventor 陈姝娟欧阳莞琳刘梦茹邹立扣敖晓琳刘书亮何利申光辉杨勇
Owner SICHUAN AGRI UNIV
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