Paper-based sensor for detecting H2O2 based on mesoporous CuO nano-enzyme, and preparation method thereof

A sensor and nanozyme technology, applied in the field of biomedical detection, can solve the problems of low portability, complicated operation, and high cost, and achieve the effects of strong universality, simple operation process, and high selectivity

Pending Publication Date: 2021-06-11
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These lead to high cost, complicated operation and low portability of traditional paper-based sensors

Method used

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  • Paper-based sensor for detecting H2O2 based on mesoporous CuO nano-enzyme, and preparation method thereof
  • Paper-based sensor for detecting H2O2 based on mesoporous CuO nano-enzyme, and preparation method thereof
  • Paper-based sensor for detecting H2O2 based on mesoporous CuO nano-enzyme, and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0037] refer to figure 2 and 3 , a mesoporous CuO-based nanozyme for the detection of H 2 o 2 The preparation method of the paper-based sensor comprises the following steps:

[0038]Step 1. Dissolve the amphiphilic block copolymer F127, tannic acid (TA), and formaldehyde in an alkaline water / ethanol mixed solvent, and stir at room temperature for 12 to 24 hours to prepare a mixed solution; add nitric acid to the mixed solution After continuing to stir the copper aqueous solution for a certain period of time, the copper-tannic acid-formaldehyde polymer is obtained by hydrothermal and centrifugal collection, and the copper-tannic acid-formaldehyde polymer is roasted in air to obtain mesoporous CuO hollow nanospheres;

[0039] Step 2. Disperse the mesoporous CuO hollow nanospheres in a certain volume of aqueous solution, soak the paper in the CuO aqueous solution for a certain period of time, let it dry naturally at room temperature, then cut the paper and store it in a plast...

Embodiment 1

[0045] A mesoporous CuO nanozyme based on the detection of H 2 o 2 The preparation method of the paper-based sensor comprises the following steps:

[0046] Step 1. Preparation of Mesoporous CuO Hollow Nanospheres

[0047] S1.1. Dissolve the amphiphilic block copolymer F127 and tannic acid in a mixed solution of alkaline water and ethanol, then add formaldehyde, and stir at room temperature for 12 to 24 hours to prepare a mixed solution;

[0048] Dissolve 0.20 g block copolymer F127 and 0.20 g tannic acid in 48 mL H 2 In the mixed solution of O and 8mL absolute ethanol, add 0.35mL concentrated ammonia water after completely dissolving, slowly add 0.38mL formaldehyde solution after stirring for 5min, and add 2mL freshly prepared Cu(NO 3 ) 2 solution (the mass of the metal salt is 0.10g), continue to stir for 12h, and then place it in an oven at 100°C for 12h;

[0049] S1.2. Collect hydrothermal products by centrifugation at 8500rpm for 10min, then centrifugally wash with ul...

Embodiment 2

[0062] A mesoporous CuO nanozyme based on the detection of H 2 o 2 The preparation method of the paper-based sensor comprises the following steps:

[0063] Step 1. Preparation of Mesoporous CuO Hollow Nanospheres

[0064] S1.1. Dissolve the amphiphilic block copolymer F127 and tannic acid in a mixed solution of alkaline water and ethanol, then add formaldehyde, and stir at room temperature for 12 to 24 hours to prepare a mixed solution;

[0065] Dissolve 0.20 g block copolymer F127 and 0.20 g tannic acid in 48 mL H 2 In the mixed solution of O and 8mL absolute ethanol, add 0.35mL concentrated ammonia water after completely dissolving, slowly add 0.38mL formaldehyde solution after stirring for 5min, and add 2mL freshly prepared Cu(NO 3 ) 2 solution (the mass of the metal salt is 0.10g), continue to stir for 12h, and then place it in an oven at 100°C for 12h;

[0066] S1.2. Collect hydrothermal products by centrifugation at 8500rpm for 10min, then centrifugally wash with ul...

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Abstract

The invention discloses a paper-based sensor for detecting H2O2 based on mesoporous CuO hollow nano-enzyme, and a preparation method thereof, and belongs to the field of biomedical detection. The specific surface area of the CuO nanosphere is 30-100 m<2> / g, the CuO nanosphere has a mesoporous and hollow structure, the mesoporous aperture size is 6 nm, and the particle size is about 100 nm. Due to the unique structure, the mesoporous CuO has high peroxidase activity. The paper-based sensor has the characteristics of simplicity in manufacturing, convenience in carrying, adjustable size, low cost, good selectivity and the like. The linear detection range is 10-150 [mu]M, the lowest detection limit can reach 2.4 [mu]M, and the paper-based sensor can be used for directly detecting trace H2O2 in the fields of chemical engineering, textile, environmental protection, medical treatment, food processing and the like.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and relates to a paper-based sensor based on mesoporous CuO nanozyme and a preparation method. Background technique [0002] h 2 o 2 As a strong oxidizing agent and bleaching agent, it is widely used in biomedicine, household and industry, but the use of hydrogen peroxide will cause serious harm to human life and health. h 2 o 2 The current detection methods mainly include electrochemical method, chromatography, fluorescence method, chemiluminescence method, colorimetric method, paper-based sensor, etc., but these methods need to use expensive and cumbersome instruments and equipment, and the cost of use is high, so there is an urgent need to develop A simple, low-cost detection method that does not require large instruments. [0003] Paper-based sensors are widely used in the field of analysis and detection, such as metal ion, DNA, protein detection, etc., because of their advantages suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78B82Y15/00B82Y40/00
CPCG01N21/78B82Y15/00B82Y40/00
Inventor 魏晶成东冯尤优秦婧冯冰溪
Owner XI AN JIAOTONG UNIV
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