Preparation method of imitated cortical bone repair material

A technology for repairing material and cortical bone, applied in the field of bone tissue reconstruction, can solve the problems of infection, failure and recurrence of surgical bone grafting, and achieve the effects of improving anti-infection efficacy, reducing damage, and strongly inhibiting and killing effects.

Inactive Publication Date: 2021-06-15
WEIFANG AOJING MEDICAL RES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difficulty lies in the possibility of infection in the surgical bone graft, so the conventional solution is to perform the surgical bone graft after at least ten months of healing of the infection after the fracture and the large bone defect left over from chronic osteomyelitis. Failure due to recurrence of infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take the long tubular bones of newly slaughtered cattle, pigs, and sheep, use a chainsaw to cut off the metaphysis on both sides, and use a bone knife to clean the surrounding soft tissue, periosteum, and bone marrow. Cancellous bone is sawn into 6.0CM×4.0CM×1.0CM block; cortical bone is sawed into 8.0CM×4.0CM×0.80CM bone plate; it can also be processed into 0.5CM×0.5CM×0.5CM small block, and the bone Put in distilled water to wash to remove surface impurities, and perform ultrasonic washing on the bone to remove the impurities on the surface and in the bone hole, and finally perform ultrasonic cleaning on the treated bone to remove the impurities on the surface and in the bone hole and dry deal with.

[0021] Put a chloroform-methanol mixed solution (referred to as degreasing solution) with a volume ratio of chloroform and methanol of 2:1 in a sealable glass container, and put the cleaned heterogeneous bone aggregates in a bone fluid volume ratio of about 1:2. Soak in...

Embodiment 2

[0028] Take the long tubular bones of newly slaughtered cattle, pigs, and sheep, use a chainsaw to cut off the metaphysis on both sides, and use a bone knife to clean the surrounding soft tissue, periosteum, and bone marrow. Cancellous bone is sawn into 6.0CM×4.0CM×1.0CM block; cortical bone is sawed into 8.0CM×4.0CM×0.80CM bone plate; it can also be processed into 0.5CM×0.5CM×0.5CM small block, and the bone Put in distilled water to wash to remove surface impurities, and perform ultrasonic washing on the bone to remove the impurities on the surface and in the bone hole, and finally perform ultrasonic cleaning on the treated bone to remove the impurities on the surface and in the bone hole and dry deal with.

[0029] Put a chloroform-methanol mixed solution (referred to as degreasing solution) with a volume ratio of chloroform and methanol of 2:1 in a sealable glass container, and put the cleaned heterogeneous bone aggregates in a bone fluid volume ratio of about 1:2. Soak in...

Embodiment 3

[0036] Take the long tubular bones of newly slaughtered cattle, pigs, and sheep, use a chainsaw to cut off the metaphysis on both sides, and use a bone knife to clean the surrounding soft tissue, periosteum, and bone marrow. Cancellous bone is sawn into 6.0CM×4.0CM×1.0CM block; cortical bone is sawed into 8.0CM×4.0CM×0.80CM bone plate; it can also be processed into 0.5CM×0.5CM×0.5CM small block, and the bone Put in distilled water to wash to remove surface impurities, and perform ultrasonic washing on the bone to remove the impurities on the surface and in the bone hole, and finally perform ultrasonic cleaning on the treated bone to remove the impurities on the surface and in the bone hole and dry deal with.

[0037] Put a chloroform-methanol mixed solution (referred to as degreasing solution) with a volume ratio of chloroform and methanol of 2:1 in a sealable glass container, and put the cleaned heterogeneous bone aggregates in a bone fluid volume ratio of about 1:2. Soak in...

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PUM

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Abstract

The invention discloses a preparation method of an imitated cortical bone repair material, which comprises the following steps: fully removing soft tissues, bone marrow and cartilage from a cleaned xenogeneic bone material, cleaning, degreasing, decalcifying, soaking a bone block in a glutaraldehyde solution for crosslinking treatment, soaking the bone block in proteolytic enzyme of pepsin, carrying out deproteinization treatment, compounding the xenogeneic bone material with a nano-silver gelatin solution, and finally freeze-drying to obtain a cortical bone grafting material. Degreasing, decalcification and freeze drying are carried out on the xenogeneic bone to assist in reducing antigenicity, reserving osteogenic activity and improving the osteogenic activity of the xenogeneic bone, deproteinization treatment is carried out through pepsin, damage to collagen and osteogenic factors in the bone grafting material can be reduced, and nano-silver micro powder is adopted as an antibacterial component, so that the bone grafting material has strong inhibiting and killing effects on various pathogenic microorganisms, and the bone grafting material forms a slow-release film, which can further improve the anti-infection efficacy of the xenogeneic bone grafting material.

Description

technical field [0001] The invention relates to the technical field of bone tissue reconstruction, in particular to a method for preparing a cortical-like bone repair material. Background technique [0002] The repair of bone defects has always been a difficult problem in orthopedic treatment due to bone defects caused by trauma and bone diseases. The source of autologous bone is limited, affecting the function of the donor site, increasing surgical trauma and infection; the source of allogeneic bone is also limited, and there is a risk of infection with HIV, hepatitis virus and other diseases. [0003] As a kind of biological tissue, xenograft bone has the advantages of wide sources and easy acquisition in large quantities. It has a natural porous network structure and good biomechanical strength, and is easier to absorb and replace than artificial synthetic bone materials. Xenogeneic bone includes bovine bone, pig bone, deer bone, sheep bone, etc., because of its size and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54A61L27/50A61L27/22A61L27/02
CPCA61L27/025A61L27/222A61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/50A61L27/54A61L2300/104A61L2300/404A61L2300/602A61L2430/02A61L2430/40
Inventor 李洪景崔孟龙刘洋仇志烨宋天喜何志敏朱艳泽崔云胡艳丽李良才朱金亮
Owner WEIFANG AOJING MEDICAL RES CO LTD
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