Mycorrhizal inoculant and preparation method thereof

A technology for mycorrhizal inoculants and strains, applied in the field of mycorrhizal inoculants and their preparation, can solve problems such as low utilization rate of chemical fertilizers, low quality survival rate of vegetables, melons and fruits, and achieve excellent traits, promote rapid reproduction, and improve survival. rate effect

Active Publication Date: 2021-06-25
上海乾界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a mycorrhizal fungal agent, which can solve the problems of low utilization rate of traditional chemical fertilizers; large-scale use of vegetables and fruits greatly reduces the quali

Method used

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  • Mycorrhizal inoculant and preparation method thereof
  • Mycorrhizal inoculant and preparation method thereof
  • Mycorrhizal inoculant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of mycorrhizal agent, comprising the following steps:

[0035] S1. Strain propagation: Mix 10 parts by weight of distilled water, 20 parts by weight of wheat bran, 6 parts by weight of ammonium molybdate, 2 parts by weight of sodium tripolyphosphate, and 3 parts by weight of glucose, sterilize at 121°C for 30 minutes, and cool Obtain the expansion medium, inoculate the bacteria and Sudan grass seeds, and culture aseptically in a plant tissue culture bottle at a temperature of 30°C for 4 weeks, and take the roots and culture medium as the expansion bacteria; the inoculation amount of the bacteria is liquid culture 1wt% of the base mass, the Sudan grass seed is 2wt% of the liquid culture medium quality;

[0036]S2. Preparation: Add the multiplication bacteria into the solid medium and mix evenly. The addition of the multiplication bacteria is 20wt% of the solid medium, sow the Sudan grass seeds, and the content of the Sudan grass seeds is the solid me...

Embodiment 2

[0039] Basically the same as Example 1, the difference is only: the vermiculite powder is replaced by activated vermiculite powder;

[0040] The activated vermiculite powder is prepared according to the following method: put 50 parts by weight of vermiculite powder into a rotating frying pan with a temperature of 70° C. and fry it at a constant temperature for 20 minutes, take it out, and immerse it in 100 parts by weight of 6wt% glucose aqueous solution for 30 minutes. Ultrasonic treatment with a power of 130W and a frequency of 25kHz for 30 minutes, filtering, placing the precipitate in a muffle furnace, heating to 800°C and calcination for 2 hours, then taking it out to obtain activated vermiculite powder.

Embodiment 3

[0042] It is basically the same as in Example 1, the only difference is that the perlite particles are replaced by activated perlite particles;

[0043] The activated perlite particles are prepared according to the following method: 50 parts by weight of perlite particles are added to 150 parts by weight of a 15% citric acid aqueous solution, ultrasonically washed at 200W and 25kHz for 2 hours, then transferred into a Buchner funnel, filtered and washed with distilled water After 30 minutes, the suction filtration product was dried and pulverized and passed through a 100-mesh sieve to obtain purified perlite particles; take all the purified perlite particles, add 150 parts by weight of an activator with a mass fraction of 3%, and immerse in a temperature of 40 ° C. Soak in heat preservation for 2 hours, filter, place the precipitate in a muffle furnace, heat up to 450°C, heat preservation and calcining for 1 hour, then take it out to obtain activated perlite particles;

[0044...

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Abstract

The invention discloses a mycorrhizal inoculant and a preparation method thereof, and belongs to the technical field of mycorrhizal inoculants. The preparation method of the mycorrhizal inoculant comprises the following steps: S1, strain propagation: uniformly mixing distilled water, bran, ammonium molybdate, sodium tripolyphosphate and glucose, sterilizing, inoculating strains and sudangrass seeds, performing sterile culture in a plant tissue culture bottle for 3-5 weeks, and taking roots and a culture medium as propagation strains; S2, preparing: adding the propagation strains into a solid culture medium, uniformly mixing, sowing the sudangrass seeds, uniformly stirring, after seedling emergence, continuously culturing for 2-3 months, cutting off the overground part of the sudangrass, cutting into root segments, uniformly mixing with a culture medium, and drying and crushing a preparation, so as to obtain the mycorrhizal inoculant. The mycorrhiza fungicide can promote plants to absorb nutrients from unweathered mineral substances, meanwhile, mycorrhiza generates various growth stimulants in the metabolic process, nutrient operation in the plants is adjusted, and then crop growth is promoted.

Description

technical field [0001] The invention relates to the technical field of mycorrhizal fungi, in particular to a mycorrhizal fungal and a preparation method thereof. Background technique [0002] As a symbiont formed by plant roots and fungi, mycorrhizal fungi have a significant impact on plant growth promotion. It affects the growth and development of plants in many ways: (1) improve the absorption of various mineral nutrients by host plants, and mycorrhizal fungi can significantly promote the absorption of nutrients in the soil by plants and improve the ability of plants to resist diseases and insect pests; (2) improve (3) Promote plant growth, increase yield and improve quality, thereby increasing economic benefits to a certain extent; (4) Enhance plant resistance to adversity. Therefore, mycorrhizal fungi play an important physiological role in the nutrient absorption and growth and development of plants such as corn and soybean. [0003] Mycorrhizal fungi can be applied t...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G5/40A01G24/25A01G24/15C12N1/14C12R1/645
CPCC05G3/00C05G5/40C05B7/00A01G24/25A01G24/15C12N1/14C05C3/00C05D9/00C05F5/002C05F11/08Y02P60/21
Inventor 林江一陈彪卢丽珍蒋蕊
Owner 上海乾界生物科技有限公司
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