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Gold silver sulfide protein composite hydrogel and preparation method and application thereof

A composite hydrogel and silver sulfide technology, which is applied in the field of nanomaterials, can solve the problems of photobleaching performance, unfavorable photothermal conversion, weakening the photothermal effect of tumor sites, and poor biocompatibility, so as to achieve high-efficiency photothermal therapy and reduce tumors. Effect of relapse, high biocompatibility

Active Publication Date: 2021-07-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PTT itself is almost non-toxic, and its main disadvantages are poor biocompatibility, low photothermal conversion efficiency, and long-term toxicity problems caused by photothermal agents (PTAs)
Among them, organic photothermal materials have good biodegradability, but photobleaching performance is not conducive to their photothermal conversion.
Conventional intravenous administration reduces the accumulation of PTAs and attenuates the photothermal effect at the tumor site
Multiple doses, especially due to fluid circulation, often lead to resistance

Method used

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  • Gold silver sulfide protein composite hydrogel and preparation method and application thereof
  • Gold silver sulfide protein composite hydrogel and preparation method and application thereof
  • Gold silver sulfide protein composite hydrogel and preparation method and application thereof

Examples

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preparation example Construction

[0048] In a specific embodiment, the preparation method of the injectable in vitro chemically synthesized protein composite photothermal gel provided by the present invention includes:

[0049] A) dissolving positively charged chitosan in acetic acid 1% (v / v) aqueous solution, and dispersing to obtain chitosan solution;

[0050] B) will positively charged Ag 3 AuS 2 NPs are mixed with chitosan solution;

[0051] C) will be negatively charged (VPEGG) 72 -GFP was dissolved in deionized water and dispersed to obtain (VPEGG) 72 -GFP aqueous solution;

[0052] D) Combine EDC, NHS and (VPGEG) 72 -GFP was mixed such that (VPEGG) 72 -The carboxyl group of GFP is activated, which is convenient for chemical cross-linking with the amino group of chitosan in the next step;

[0053] E) Ag 3 AuS 2 The mixture of NPs and chitosan and the activated (VPEGG) 72 -GFP was mixed, stirred at 60°C for 15min, and the amino groups of chitosan and (VPEGG) were used 72 -Cross-linking of the c...

Embodiment 1

[0066] Embodiment 1: (VPEGEG) 72 -Expression and purification of GFP

[0067] Step 1: Obtain Stable Expression (VPEGG) 72 -GFP strains. Use (VPGEG) 72 -GFP protein gene sequence to construct pET25b prokaryotic expression vector, then transform E.coli BLR (DE3) Escherichia coli, and induce expression screening to obtain stable expression strains.

[0068] Step 2: (VPGEG) 72 -Expression of GFP. The strains were cultured in LB medium and then inoculated into TB medium. After reaching the exponential growth phase, IPTG was added to induce protein expression at 28.5°C for 12 hours, washed by centrifugation, collected and stored at -80°C.

[0069] Step 3: (VPGEG) 72 - Purification of GFP. After the bacteria are crushed by high pressure, centrifuge to take the supernatant, filter and sterilize, and finally use a protein purifier to purify through a nickel affinity chromatography column, a desalting column, and a Q ion exchange chromatography column, and finally obtain (VPGEG) ...

Embodiment 2

[0070] Example 2: Synthesis of positively charged gold silver sulfide nanoparticles

[0071] Step 1: Preparation of heterostructured gold-silver sulfide nanomaterials (Ag 3 AuS 2 NPs). Specifically as follows: take HAuCl 4 4H 2 O 20mg, AgNO 3 Add 40 mg to a 100 mL round bottom flask, then add 10 mL oleic acid and 20 mL oleylamine, heat up to 55°C, and stir for 20 h. Take 25 mg of sublimed sulfur and add it to 10 mL of oleylamine. After ultrasonically mixing, add it to the above solution and stir at 55°C for 2 hours. After washing with ethanol for 3 times, the gold-silver sulfide nanomaterial (Ag 3 AuS 2 NPs) were dispersed in 5 mL of chloroform.

[0072] The above preparation process can refer to the attached figure 1 .

[0073] The second step: through ligand exchange, the surface oleic acid and oleylamine molecules are replaced by cetyltrimethylammonium bromide (CTAB). The details are as follows: Take 0.5g of CTAB and add it to a 30ml glass bottle, add 10ml of dei...

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Abstract

The invention relates to the technical field of a nano material, and particularly relates to gold silver sulfide protein composite hydrogel and a preparation method and application thereof. The hydrogel provided by the invention comprises a protein with negative charges, photo-thermal heterogeneous nanoparticles with positive charges and chitosan (CS) with positive charges, wherein the photo-thermal heterogeneous nanoparticles with the positive charges are gold silver sulfide hybrid nanoparticles (Ag3AuS2NPs); and the protein with the negative charges is a compound protein (VPGEG) 72-GFP of an elastin-like protein and a green fluorescent protein. The hydrogel has injectability, an efficient photo-thermal treatment effect and biocompatibility, and the photo-thermal efficiency can reach 39.0%. The hydrogel is uniformly dispersed around a tumor in a peritumoral injection manner, and then photo-thermal conversion is generated through irradiation, so that effective killing on the tumor is implemented, and tumor recurrence is reduced.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a silver gold sulfide protein composite hydrogel and a preparation method and application thereof. Background technique [0002] Malignant tumors are one of the great threats to human life and health. With the advancement of medicine, various diseases have been successfully overcome, and the life expectancy of human beings has gradually increased. However, malignant tumors are still persistent diseases that cannot be overcome by the world's medical circles. Tongue cancer is the most common malignant tumor of the oral cavity, with a high degree of malignancy and a high rate of metastasis. The current treatment of tongue cancer is surgery-based comprehensive treatment, including radiotherapy and chemotherapy. However, when the tumor invades deeply, hemilingual or total tongue resection is often required, which severely disrupts motor, sensory, and language functions. Chemo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/06A61K47/36A61K47/42A61P35/00
CPCA61K41/0052A61K9/06A61K47/36A61K47/42A61P35/00
Inventor 刘凯张洪杰
Owner TSINGHUA UNIV
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