Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A dual-signal detection method for Staphylococcus aureus in food

A staphylococcus and detection method technology, applied in the field of microbial detection, can solve problems such as unfavorable detection promotion, expensive laboratory facilities, complex pretreatment procedures, etc., to ensure food safety, wide concentration detection range, and eliminate background fluorescence and other ions. the effect of interference

Active Publication Date: 2022-07-29
JIANGSU UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these techniques have high sensitivity and effectiveness, there are some inherent drawbacks, such as expensive laboratory facilities, complex pretreatment procedures and the need for trained staff, which are not conducive to the implementation of on-site detection and in the Promotion in practical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A dual-signal detection method for Staphylococcus aureus in food
  • A dual-signal detection method for Staphylococcus aureus in food
  • A dual-signal detection method for Staphylococcus aureus in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 The dual-signal detection method of Staphylococcus aureus in pork, the concrete steps are:

[0044]Step 1, preparation of water-soluble up-conversion nanomaterials: accurately weigh 0.1183g yttrium chloride hexahydrate, 0.0388g ytterbium chloride hexahydrate and 0.0038g erbium chloride hexahydrate, dissolve them with 10mL of methanol ultrasonically for 10min, and transfer them into a 250mL three-necked round-bottomed flask containing 6mL of oleic acid and 10mL of 1-octadecene (oleic acid: 1-octadecene=3:5); under argon protection, magnetic stirring and heating to 160°C, After maintaining for 40min to form a transparent solution, it was naturally cooled to room temperature; then, a 10mL methanol mixture dissolved with 50mg sodium hydroxide (1.25mmol) and 70mg ammonium fluoride (2mmol) was added dropwise to the above flask to form a white suspension. The turbid liquid was then heated to 45 °C and kept for half an hour under closed conditions; then, the device...

Embodiment 2

[0053] Embodiment 2 The double signal detection method of Staphylococcus aureus in beef, concrete steps are:

[0054] Step 1, preparation of water-soluble up-conversion nanomaterials: accurately weigh 0.1183g yttrium chloride hexahydrate, 0.0388g ytterbium chloride hexahydrate and 0.0038g erbium chloride hexahydrate, dissolve them with 10mL of methanol ultrasonically for 10min, and transfer them into a 250mL three-necked round-bottomed flask containing 6mL of oleic acid and 14mL of 1-octadecene (oleic acid: 1-octadecene=3:7); under argon protection, magnetic stirring and heating to 160°C, After maintaining for 40min to form a transparent solution, it was naturally cooled to room temperature; then, 10 mL of methanol mixture dissolved with 50 mg of sodium hydroxide (1.25 mmol) and 74.1 mg of ammonium fluoride (2 mmol) was added dropwise to the above flask to form a white The suspension was then heated to 45 °C for half an hour under closed conditions; then, the device was turned...

Embodiment 3

[0063] Embodiment 3 The double-signal detection method of Staphylococcus aureus in milk, concrete steps are:

[0064] Step 1, preparation of water-soluble up-conversion nanomaterials: accurately weigh 0.1183g yttrium chloride hexahydrate, 0.0388g ytterbium chloride hexahydrate and 0.0038g erbium chloride hexahydrate, dissolve them with 10mL of methanol ultrasonically for 10min, and transfer them to a 250mL three-necked round-bottomed flask containing 6mL of oleic acid and 20mL of 1-octadecene (oleic acid: 1-octadecene=3:10); under argon protection, magnetic stirring and heating to 160°C, After maintaining for 40min to form a transparent solution, it was naturally cooled to room temperature; then, 10mL methanol mixture dissolved with 50mg sodium hydroxide (1.25mmol) and 75mg ammonium fluoride (2mmol) was added dropwise to the above flask to form a white suspension. The turbid liquid was then heated to 45 °C and kept for half an hour under closed conditions; then, the device was...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a dual-signal detection method for Staphylococcus aureus in food, and belongs to the technical field of microorganism detection. The detection method comprises the following steps: modifying the water-soluble up-conversion nanomaterial by using the complementary chain of Staphylococcus aureus aptamer and horseradish peroxidase to obtain a color probe; using the Staphylococcus aureus aptamer to modify amino groups Functionalizing magnetic nanoparticles to obtain aptamer-modified magnetic nanoparticles as capture probes; mixing and incubating the chromogenic probe and the capture probe to obtain a dual-signal molecule-magnetic nanoparticle complex system, namely for a specific detection system. The specific dual-signal detection system established by the invention has a wide linear detection range, the detection limits of fluorescence intensity and absorbance signal LOD are 22 CFU / mL and 20 CFU / mL respectively, with high sensitivity, and can specifically detect Staphylococcus aureus in food .

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a dual-signal detection method of Staphylococcus aureus in food. Background technique [0002] As a food-borne pathogen, Staphylococcus aureus can cause food poisoning through food contamination at any stage of the food production to consumption process. Staphylococcus aureus accounted for 12.5% ​​of foodborne bacterial outbreaks in China and was the third most common foodborne pathogen after Vibrio parahaemolyticus and Salmonella. It can cause many diseases, from minor skin and soft tissue infections to life-threatening conditions such as dermatitis, gastrointestinal infections, bacteremia, and infective endocarditis. my country's national standard also clearly stipulates that the detection amount of Staphylococcus aureus in pork is 100CFU / g-1000CFU / g. However, traditional detection methods, such as plate counting method, polymerase chain reaction, etc., not only have a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/31
CPCG01N21/6486G01N21/31
Inventor 欧阳琴王丽陈全胜杨永存李欢欢吴继忠郭志明赵杰文
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com