Fluorescent sensor for detecting respiratory ammonia as well as preparation method and application of fluorescent sensor

Abstract

The present invention provides a fluorescent sensor for detecting respiratory ammonia. A curcumin derivative (BFCUR) having a large steric hindrance and electrospun nanofibers (ENF) having a high porosity and a high specific surface area are compounded. The BFCUR with large steric hindrance is used as a sensing fluorophore, so that aggregation caused by pi-pi accumulation is effectively avoided; and the ENF with high porosity and high specific surface area can improve the sensitivity of the sensor. The sensor provided by the invention shows excellent performance in detection of respiratory ammonia, has ultralow detection limit, wider ammonia gas concentration response range and short response time, is slightly interfered by carbon dioxide and water vapor, and is high in cycling stability and light stability and good in recoverability. The sensor disclosed by the invention is successfully applied to respiratory ammonia detection of patients with chronic kidney diseases and helicobacter pylori infection, is high in sensitivity and reliable in detection result, and has important significance in clinical practice.

Description

technical field [0001] The invention belongs to the technical field of gas sensors, in particular to a fluorescent sensor for detecting breath ammonia, a preparation method and application thereof. Background technique [0002] Concentrations of volatile organic compounds (VOCs) in human breath have been shown to be associated with human pathology. Among them, the level of ammonia in exhaled breath has been shown to be closely related to various diseases, including chronic kidney disease (CKD) and Helicobacter pylori (H. pylori) infection. Among them, respiratory ammonia, a by-product of urea metabolism in CKD patients, is closely related to two blood markers, creatinine and blood urea nitrogen. The respiratory ammonia level of healthy people is 50-1500ppb, while that of CKD patients will increase to 15000ppb. However, the increased urease in Helicobacter pylori infection may be further decomposed into CO 2 and NH 3 , so breath ammonia levels can also be used as an indic...

AI Technical Summary

Problems solved by technology

However, these methods often exhibit some disadvantages: Although GC-MS can detect ammonia with high sensitivity, the pretreatment process of gas collection is complicated, so that it can only be limited to the laboratory

However, due to the strong π-π stacking interaction of curcumin molecules triggering the aggregation fluorescence quenching effect (ACQ), it is difficult to generate solid-state fluorescence that can be used for breath ammonia detection.

In addition, the low specific surface area of ​​the sensing matrix also hinders the high sensitivity and reliability of in vitro and in vivo bioassays.

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