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Preparation method of antibacterial glycopeptide hydrogel

A hydrogel and antibacterial sugar technology, applied in medical science, bandages, etc., can solve the problems that hinder the wide application of antibacterial hydrogels, the degradation cycle of antibacterial peptides is short, and the quaternary ammonium salt cytotoxicity is high, so as to induce autoimmunity, The effect of promoting wound healing and accelerating wound healing

Active Publication Date: 2021-08-06
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used inorganic antibacterial agents are silver particles or nanosilver. Although the antibacterial hydrogel containing nanosilver has good antibacterial activity, nanosilver is easy to accumulate in the body and difficult to excrete, resulting in potential biosafety, hindering Wide application of this type of antibacterial hydrogel
Organic antibacterial agents include traditional antibiotics, quaternary ammonium salts, etc. Antibacterial hydrogels containing antibiotics can be used to prevent infection, but antibiotics can easily lead to bacterial resistance
Quaternized chitosan hydrogel is another commonly used external antibacterial biological product, which has a broad-spectrum antibacterial effect and will not produce drug resistance; however, quaternary ammonium salts are highly toxic and will damage them, which is not conducive to Cell Proliferation
[0005] The currently used antibacterial peptides have a short degradation cycle, are easy to degrade, and have high cytotoxicity, which limits their application; properties, viscoelasticity, bioactivity and degradability; collagen peptide is an essential substance for wound repair

Method used

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  • Preparation method of antibacterial glycopeptide hydrogel
  • Preparation method of antibacterial glycopeptide hydrogel
  • Preparation method of antibacterial glycopeptide hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 discloses a method of preparing an antibacterial peptide hydrogel.

[0051] A method for preparing a hydrogel of antibacterial peptide peptide, including the following steps:

[0052] S1, dissolve the glucosaccharide and the molar ratio of 5: 1 in distilled water according to the molar ratio of 5: 1, reacting under the protected light, and the addition of excess ethylene glycol stopped, dialysis 48h, lyophilized 48 h, to obtain oxidation Gliton (GM-CHO), dissolved GM-CHO with the antibacterial peptide in distilled water at a molar ratio of 5: 1, reacted at room temperature for 48 h, resulting in antibacterial peptide glycan;

[0053] S2, the hyaluronic acid, 3-maleimide propionic acid is dissolved in distilled water according to the molar ratio of 1: 2, and the catalyst 1- (3-dimethylaminopropyl) 3-ethyl carbon is added thereto. Diimine hydrochloride and 4-dimethylpyridine, at room temperature for 48 h, then lysis, lysis, to obtain esterification hyaluronic acid (...

Embodiment 2

[0056] Example 2 A method of preparing an antimicrobial peptide hydrogel is disclosed.

[0057] A method for preparing a hydrogel of antibacterial peptide peptide, including the following steps:

[0058] S1, dissolve the glucan with high-iodide in distilled water in distilled water according to the molar ratio of 10: 1, and the reaction of excess ethylene glycol is added, 48 h, lyophilized for 48 h, to give GM- CHO, dissolved GM-CHO with the antibacterial peptide in distilled water at a molar ratio of 10: 1, reacted at room temperature for 48 h, then dialyzes lyophilized to give antibacterial peptide glycan;

[0059] S2, the hyaluronic acid, 3-maleimide propionic acid is dissolved in distilled water according to the molar ratio of 1: 1, and the catalyst 1- (3-dimethylaminopropyl) 3-ethyl carbon is added thereto. Dismine hydrochloride and 4-dimethyl amino pyridine react at room temperature for 48 h, to obtain HA-COO, dissolve HA-COO and collagen in distilled water, the molar ratio ...

Embodiment 3

[0062] Example 3 discloses a method of preparing an antibacterial peptide hydrogel.

[0063] A method for preparing a hydrogel of antibacterial peptide peptide, including the following steps:

[0064] S1, dissolve the glucosaccharide with the molar ratio of the sodium of 6: 1 in distilled water in distilled water, and the addition of excess ethylene glycol stopped, dialysis 48h, lyophilized 48 h, resulting in oxidation G. G. Glitrose, the antibacterial peptide was dissolved in distilled water in distilled water at 6: 1, at room temperature to obtain antibacterial peptide glycan;

[0065] S2, the hyaluronic acid, 3-maleimide propionic acid is dissolved in distilled water according to the molar ratio of 1: 1.5, and the catalyst 1- (3-dimethylaminopropyl) 3-ethyl carbon is added thereto. Diimine hydrochloride and 4-dimethylhydridine, at room temperature for 48 h, dialysis 48h, lyophilized 72 h, to obtain HA-COO, dissolve HA-COO and collagen in distilled water, molar ratio is 6: 1 The...

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Abstract

The invention discloses a preparation method of antibacterial glycopeptide hydrogel, the preparation method comprises the following specific steps: taking gulcomannan to react with sodium periodate, adding ethylene glycol to terminate the reaction to obtain oxidized gulcomannan, dialyzing and freeze-drying, and mixing with antibacterial peptide to react to obtain antibacterial peptide gulcomannan; reacting hyaluronic acid with 3-maleimidopropionic acid at room temperature to obtain esterified hyaluronic acid, dialyzing, freeze-drying, mixing with collagen peptide, carrying out addition reaction, dialyzing and freeze-drying to obtain collagen peptide hyaluronic acid; mixing the collagen peptide hyaluronic acid and the antibacterial peptide glucomannan to prepare the antibacterial glycopeptide hydrogel. The prepared hydrogel has good antibacterial performance on microbial bacteria such as gram-positive bacteria, gram-negative bacteria, fungi and the like, and the hydrogel is simple in preparation process, degradable and good in biocompatibility.

Description

Technical field [0001] The present invention relates to the field of biomedical materials and related medical equipment R & D technology, and more particularly to a glycopeptide hydrogel having a combination of antibacterial repair properties and preparation methods and applications. Background technique [0002] With the implementation of the Health China 2030 strategy, the economic transformation of the national health industry is more and more concerned about health problems. The relevant medical problems caused by bacterial infections have become a hot spot in clinical research. Infection is one of the severe complications of trauma and other open wounds, and the wound caused by bacterial infections is a major clinical issue. Bacterial drug resistance caused by antibiotic abuse brings great difficulties. With the rapid development of materials and engineering technologies, many new medical antibacterial applications come into born, impart certain antibacterial properties to b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L5/08C08L5/00A61L26/00
CPCC08J3/075A61L26/008A61L26/0066A61L26/0052A61L26/0085A61L26/009C08J2305/08C08J2405/00A61L2300/252A61L2300/236A61L2300/232A61L2300/404A61L2300/412A61L2300/41A61L2300/602C08L89/00C08L5/02C08L5/08
Inventor 王伟伟刘文帅冯祖建黄平升孔德领张闯年
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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