Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Plant growth-promoting microbial inoculum for tailing restoration as well as preparation method and application of plant growth-promoting microbial inoculum

A technology of plant growth-promoting bacteria and tailings, applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problems of high cost, insignificant long-term effect, cumbersome application methods, etc., and achieve improved Plant height, increase soil nutrient content and microbial quantity, beneficial to preservation and application

Pending Publication Date: 2021-08-10
NANCHANG UNIV
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, PGPB bacterial agents are used to strengthen phytoremediation of tailings soil, but there are problems such as cumbersome application methods, high costs, and insignificant long-term effects.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant growth-promoting microbial inoculum for tailing restoration as well as preparation method and application of plant growth-promoting microbial inoculum
  • Plant growth-promoting microbial inoculum for tailing restoration as well as preparation method and application of plant growth-promoting microbial inoculum
  • Plant growth-promoting microbial inoculum for tailing restoration as well as preparation method and application of plant growth-promoting microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of plant growth promoting agent

[0032](1) Screening and cultivation of member strains of plant growth-promoting functional flora: Based on the principle of complementary functions, rich diversity, consistent growth rules and no antagonism between strains, the member strains used to construct the flora are screened out: Burkholderia catarinensis, Burkholderia cenocepacia, Burkholderiaseminalis, Burkholderia ubonensis, Burkholderia puraquae, Burkholderia anthina, Paraburkholderia fungorum, Klebsiella luumibacterium michiganensis, Achromobacter insuumactirquavis, Herba spirulina . First, streak on a 0.1×TSB plate and place it in a constant temperature incubator at 28°C for 24-48h. Afterwards, a single colony was picked and placed in 5 ml of TSB medium, and cultured overnight on a shaker at 28°C. Then transfer 50 μl of the culture to a new 5 mL TSB medium and incubate at 28°C for 8 h on a shaker. Determination of bacterial solution OD 600...

Embodiment 2

[0039] Embodiment 2: application method and plant cultivation of plant growth-promoting bacterium agent

[0040] (1) Soil for testing: the rare earth tailings soil collected from Longnan County, Ganzhou City, was naturally air-dried and passed through a 2mm sieve for use. The soil is red and sandy, with high sand content, large pores, low water content and weak adhesion. The pH value of the soil in the study plot is 4.06±0.07, which belongs to acidic soil. The soil is poor in nutrition, the total nitrogen is 0.052±0.008g / kg, the total phosphorus is 0.043±0.003g / kg, and the total organic carbon content is 0.53±0.01g / kg. The soil was polluted by three heavy metals, Cd, Pb and Zn, the total contents were 3.50±0.14mg / kg, 425±14.43mg / kg and 143.50±7.79mg / kg, respectively.

[0041] (2) Plants to be tested: Wujiemang, a typical restoration plant of rare earth tailings.

[0042] (3) Bacteria application: inoculate the plant growth-promoting bacterium prepared in Example 1 with the ...

Embodiment 3

[0044] Example 3: Plant growth-promoting bacteria agent strengthens the effect of phytoremediation of rare earth tailings soil

[0045] The plant height was measured after the plants grew to 45 days. The plants are then removed from the soil, keeping the roots as intact as possible. Carefully clean the roots with tap water, blot the excess water on the plant surface with absorbent paper, separate the shoots and roots of the plants, measure and record their fresh weight and dry weight respectively. At the same time, the collected soil was divided into two parts: the first part was stored in a refrigerator at 4°C for the determination of soil pH, ammonium nitrogen content, nitrate nitrogen content, microbial biomass carbon, colony count and preservation for future use. The second part is used for the total nitrogen content, total phosphorus content and total organic carbon content of the soil after air drying.

[0046] (1) Effects of plant growth-promoting agents applied in ra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
heightaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a plant growth-promoting microbial inoculum for tailing restoration, which is prepared by taking a plant growth-promoting functional flora as a main body and taking corn straw biochar serving as an adsorbent, polyvinyl alcohol serving as an embedding agent, sodium alginate and Ca (NO3)2 serving as a cross-linking agent for fixing. The member strains are complementary in function, high in diversity and consistent in growth rule, and mutual antagonism among the strains does not exist. The invention provides a preparation method of the plant growth-promoting microbial inoculum and an application of the plant growth-promoting microbial inoculum in strengthening phytoremediation of rare earth tailing soil. The microbial inoculum has multiple properties of fixing carbon, fixing nitrogen, dissolving phosphorus, supplying iron, retaining water, improving plant stress resistance, promoting plant growth and the like, and can remarkably improve the biomass, nutrient absorption, soil nutrients and the number of microorganisms of rare earth tailing plants. The microbial inoculum is applied in a granular form, is beneficial to storage and application, and is relatively high in practicability. The product has the characteristics of comprehensive performance, long-acting broad spectrum, simplicity, safety, easiness in storage and the like, and is suitable for multiple stress tailing environments such as soil acidification, water deficiency, nutrient depletion and heavy metal pollution.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and environmental restoration, and relates to a microbial agent, in particular to a microbial agent capable of improving stress resistance of tailings plants and promoting plant growth, and a preparation method and application thereof. Background technique [0002] Tailings is a site with extremely harsh environment, which has the characteristics of high concentration of heavy metal residues, extreme acidity, lack of a large number of nutrients, and poor water holding capacity. Phytoremediation is an important way to restore and rebuild tailings degraded ecosystems, but the multiple stresses of tailings soil often lead to problems such as difficulty in survival or slow growth of newly planted plants, small plant biomass, and poor stress resistance of key species, which seriously restrict tailings vegetation restoration and rebuilding process. Therefore, improving plant stress resistance and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/084C12N11/04C12N1/20A01N63/20A01P21/00C09K17/40C09K17/42B09C1/10C09K101/00C09K109/00
CPCC12N11/14C12N11/10C12N11/084C12N11/04C12N1/20A01N63/20C09K17/40C09K17/42B09C1/10B09C1/105C09K2101/00C09K2109/00Y02P60/21Y02A40/22
Inventor 孔召玉黄少仪贺勇简文佳吴兰
Owner NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com