Beta-caryophyllene transdermal absorption enhancer and application of beta-caryophyllene transdermal absorption enhancer in external preparations and cosmetics
A technology of absorption enhancer and external preparation, applied in the field of biomedicine, can solve the problem of high cytotoxicity, achieve the effect of strong promoting ability, improving effect, and promoting the penetration of skin stratum corneum barrier
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Embodiment 1
[0014] Embodiment 1 Fourier transform attenuated total reflection infrared spectrometry (ATR-FTIR) measures the effect on skin stratum corneum
[0015] Cut the rat skin to about 1 cm 2 The small pieces were placed in 5% 1,8-cineole, 5% β-caryophyllene or 5% azone solution, and the solvent was propylene glycol:isopropanol (3:7, v / v). The solvent-treated skin samples were used as the solvent group, and the untreated skin samples were used as the blank group. They were placed at 37°C for 36 hours, and then the treated skin samples were washed with distilled water and dried with filter paper. Infrared spectra of skin samples were obtained using an ATR-FTIR spectrometer. Infrared detection conditions: resolution 2cm -1 , an average of 64 scans, the scan range is 650-4000cm -1 . Table 1 shows the infrared spectrum parameters of rat skin treated with different transdermal absorption enhancers (PE).
[0016] Table 1 Infrared spectrum parameters of skin samples treated with β-cary...
Embodiment 2
[0021] Embodiment 2 skin resistance dynamics measurement
[0022] The rat skin was taken out and thawed naturally at room temperature, and hydrated in PBS solution for 1 h, and fixed on a 24-well nested plate with the cuticle facing upward. Add 650 μL of PBS solution to the receiving chamber, add 200 μL blank solvent to the supply chamber, and read the initial value of skin resistance after equilibrating at 37°C for 2 minutes, which is recorded as SER 0 . Remove the upper layer of the skin solution, add 200 μL of the test solution (containing 5% transdermal absorption enhancer), read the skin resistance value at different time points, and record it as SER t . All of the above solutions were maintained at the desired temperature.
[0023] The resistance reduction factor (RF), defined as the initial SER 0 with the resistor value SER at a predetermined time t Ratio. The calculation is as follows: RF=SER 0 / SER t . The value of RF is plotted against time t, and the slope ...
Embodiment 3
[0024] Embodiment 3 skin cytotoxicity assay
[0025] Human keratinocyte HaCaT was prepared in DMEM medium (containing 10% fetal bovine serum and 100 U·mL -1 penicillin-streptomycin solution) at 37°C in a concentration of 5% CO 2 in the incubator. MTT experiment procedure: Take HaCaT cells in the logarithmic growth phase to make a single cell suspension, adjust the cell concentration (5000 cells / well), inoculate in a 96-well culture plate, set 5 replicate wells in each group, and store in 5% CO 2 and cultured overnight at 37°C. The medium was aspirated and discarded, and 100 μL of transdermal penetration enhancer solutions with different concentrations were added, and the solvent was DMEM medium containing 1% dimethyl sulfoxide (DMSO). The culture was continued for 12 h at 37°C. Remove the medium, add 150 μL of DMSO solution, shake and dissolve for 10 minutes, detect the absorbance (A) of each well at 570 nm with a microplate reader, and calculate the cell survival rate, IC...
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