Preparation and application of black phosphorus nanosheet loaded indocyanine green nano system

A technology of indocyanine green and nanosheets, which is applied in the field of clinical imaging of diagnostic agents, can solve the problems of reducing the effect of fluorescence imaging and poor stability, and achieves the effect of simple and easy operation, mild preparation conditions, and good biodegradability

Active Publication Date: 2021-08-27
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, indocyanine green has poor stability in polar dispersions, and it is easy to aggregate to form polymers, thereby reducing the effect of fluorescence imaging. Second, the polymers are quickly cleared in plasma

Method used

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  • Preparation and application of black phosphorus nanosheet loaded indocyanine green nano system
  • Preparation and application of black phosphorus nanosheet loaded indocyanine green nano system
  • Preparation and application of black phosphorus nanosheet loaded indocyanine green nano system

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Nitrogen gas is introduced into the stripped black phosphorus suspension and kept in this state for 30 minutes to remove oxygen. Use a domestic high-speed centrifuge to screen out the black phosphorus nanosheet precipitation of this size at 2500rpm. After washing three times with double distilled water and freeze-drying, the obtained black phosphorus nanosheets were dispersed in ethanol, and the particle size was determined by transmission electron microscopy. The result is as figure 1 As shown, the size of the prepared black phosphorus nanosheets is 892±16nm

[0041] 2. Weigh 25 mg of the obtained black phosphorus nanosheets, disperse them into 25 mL of normal saline, and place them in a 50 mL Shrek bottle. Nitrogen gas was blown into the air, and the state was maintained for 30 minutes, and sealed with a rubber stopper. Then place it in a water bath at 25° C. for ultrasonication for 2 hours, so that the black phosphorus nanosheets are uniformly dispersed. Then ...

Embodiment 2

[0043] 1. Nitrogen gas is introduced into the stripped black phosphorus suspension and kept in this state for 30 minutes to remove oxygen. Use a domestic high-speed centrifuge to screen out black phosphorus nanosheets of this size at 7500rpm. After washing three times with double distilled water and freeze-drying, the obtained black phosphorus nanosheets were dispersed in ethanol, and the particle size was determined by transmission electron microscopy. The result is as image 3 As shown, the size of the prepared black phosphorus nanosheets is 312±12nm.

[0044] 2. Weigh 25 mg of the prepared black phosphorus nanosheets, disperse them into 25 mL of normal saline, and place them in a 50 mL Shrek bottle. Nitrogen gas was blown into the air, and the state was maintained for 30 minutes, and sealed with a rubber stopper. Then place it in a water bath at 25° C. for ultrasonication for 2 hours, so that the black phosphorus nanosheets are uniformly dispersed. Then slowly inject 2....

Embodiment 3

[0046] Weigh a certain amount of ICG@BPNS-PEG solid and free indocyanine green screened at different rotation speeds, and keep the concentration of indocyanine green at 2 mg / mL. Subcutaneous injection of 2*10 5 4T1 breast cancer cells, when the tumor volume reaches 50mm 3 (tumor volume calculation formula: tumor length × (tumor width) 2 / 2), two groups of mice were injected with ICG@BPNS-PEG screened at different rotational speeds into the tail vein as the experimental group, and the remaining group of mice were injected with free indocyanine green through the tail vein as the control group. After 6 hours, take pictures and observe under the small animal imager,

[0047] Compared figure 1 and Figure 4 As a result, the size of the black phosphorus nanosheets screened by high-speed centrifugation is smaller, and the black phosphorus nanosheets also have the structural characteristics of thin layers. The small size endows the corresponding black phosphorus nanosheets with hi...

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Abstract

The invention discloses preparation and application of a black phosphorus nanosheet loaded indocyanine green nano system, black phosphorus nanosheets with different sizes are used as carriers, the specific surface area of the black phosphorus nanosheets is large, and high loading of indocyanine green can be effectively achieved; in addition, through modification of aminated polyethylene glycol, the dispersion stability of the constructed nano material in a polar dispersion liquid is greatly improved. The prepared black phosphorus nanosheet loaded indocyanine green nano system can be used as a fluorescence imaging agent, tumor tissue imaging can be effectively achieved, the tumor boundary position can be distinguished, and the fluorescence imaging effect of the tumor part is enhanced.

Description

technical field [0001] The invention belongs to the technical field of clinical imaging of diagnostic agents, and in particular relates to the preparation and application of a black phosphorus nanosheet-loaded indocyanine green nanosystem. Background technique [0002] Fluorescence imaging technology is an imaging technology based on injecting fluorescent molecules into animals or humans and detecting emitted light waves to visualize tissues or organs. As a clinical complementary imaging technology, the advantages of fluorescence imaging are: first, it does not use any dangerous ionization or radiation, and second, the imaging equipment is cheap, easy to operate, and the sampling time is short, enabling real-time intraoperative imaging. [0003] Indocyanine Green, also known as ICG, is a tricarbonate hydrogen dye with the maximum absorption wavelength and emission wavelength at 740nm and 800nm, which has the advantage of strong tissue penetration, and has been approved by th...

Claims

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Application Information

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IPC IPC(8): A61K49/00B82Y5/00
CPCA61K49/0034A61K49/005A61K49/0054B82Y5/00
Inventor 胡源马小鹏陈韦剑潘婉婉
Owner UNIV OF SCI & TECH OF CHINA
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