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Copper nanocluster with various mimic enzyme activities and preparation method and application thereof

A technology of copper nano and clusters, applied in chemical instruments and methods, analyzing materials through chemical reactions, and analyzing materials through observing the influence of chemical indicators, can solve the problems of non-selectivity and achieve The preparation method is simple, the stability is good, and the effect of broad development prospects

Active Publication Date: 2021-08-27
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Ascorbic acid is necessary for maintaining immune function, hydroxylating 5-hydroxytryptamine, maintaining the integrity of blood vessels, and promoting the absorption of non-heme iron. At present, nanomaterials often use its imitation peroxidase activity to establish a colorimetric method to detect ascorbic acid, but this method not selective

Method used

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  • Copper nanocluster with various mimic enzyme activities and preparation method and application thereof
  • Copper nanocluster with various mimic enzyme activities and preparation method and application thereof
  • Copper nanocluster with various mimic enzyme activities and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0071] Add cysteine ​​hydrochloride (10mM, 20mL) dropwise to a copper sulfate solution containing copper ions (100mM, 2mL) at room temperature. After stirring for 15 minutes, add hydrazine hydrate (50μL), and continue stirring for 1.5 hours. have to.

Embodiment 2

[0073] Validation of Mimic Peroxidase Activity of Copper Nanoclusters:

[0074] Experimental system a: The catalytic reaction system is composed of acetate buffer solution (pH 4, 100mM), organic chromogen TMB (1mM) and hydrogen peroxide (1mM) after the copper nanoclusters (0.182mM) obtained in the above example. Then react in a water bath at 37° C. for 20 minutes, and measure its visible absorption spectrum in the range of 400-800 nm with a microplate reader.

[0075] In addition, do control experiment b: Include acetate buffer (pH 4, 100mM) and copper nanoclusters (0.182mM) in the catalytic system, and the rest of the reaction conditions are the same as the above-mentioned experimental system, and the absorbance value is measured after 20 minutes . Another control experiment c: no copper nanoclusters were added to the catalytic reaction system, and the absorbance value was detected after reacting for 20 minutes under the same conditions as the above-mentioned experimental sy...

Embodiment 3

[0078] Validation of Mimic Catalase Activity of Copper Nanoclusters:

[0079] Experimental system b: The catalytic reaction system contains phosphate buffer (pH 7.8, 100mM) and H 2 o 2 (5mM), the above copper nanoclusters (1.82mM), reacted at 37°C for 30 minutes, took the supernatant, then added horseradish peroxidase (25μg / mL) and ABTS (0.02mM), at room temperature After reacting for 10 minutes, record its visible absorption spectrum in the range of 380-500 nm with a UV spectrophotometer.

[0080] Another control experiment system a: the catalytic reaction system is composed of phosphate buffer (pH 7.8, 100mM), H 2 o 2 (5mM), take its supernatant, react at 37°C for 30 minutes, then add horseradish peroxidase (25μg / mL) and ABTS (0.02mM), react at room temperature for 10 minutes and record with a UV spectrophotometer Its visible absorption spectrum in the range of 380-500nm.

[0081] Such as image 3 As shown, the absorbance value of the control system b at 417nm is lower...

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Abstract

The invention provides a copper nanocluster with various mimic enzyme activities and a preparation method and application thereof, and belongs to the technical field of nano materials, biological catalysis and analysis and detection. The invention discloses the copper nanocluster with mimic peroxidase activity, mimic catalase activity, mimic superoxide dismutase activity and mimic ascorbic acid oxidase activity for the first time, and the copper nanocluster is simple in preparation method and good in stability. The copper nanocluster has a wide development prospect in the aspects of biochemical analysis, nano mimic enzyme catalysis, clinical medicine detection and the like, and has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, biocatalysis, and analysis and detection, and in particular relates to a copper nanocluster with various enzyme-like activities and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Nanomaterials, as efficient enzyme-mimicking catalysts, have been widely used in the fields of biocatalysis, nanomedicine, and treatment, showing the strong vitality of nano-mimicking enzymes. Compared with natural enzymes, artificial mimic enzymes are low in cost, easy to produce on a large scale, stable in nature, and can be widely promoted. [0004] At present, the si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/72B01J35/02G01N21/64G01N21/78
CPCB01J23/72G01N21/6428G01N21/78G01N2021/6439B01J35/394B01J35/50B01J35/40Y02A50/30
Inventor 刘爱骅刘重阳蔡园园
Owner QINGDAO UNIV
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