Zearalenone lactone hydrolase mutant S162P with improved thermal stability and application thereof

A technology of giaralenone lactone and hydrolase, applied in the field of enzyme engineering, can solve the problems of secondary pollution, low removal efficiency, unfavorable environmental protection, animal and plant health, etc., and achieve the effect of improving thermal stability
CN113308449AActive Publication Date: 2021-08-27JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-08-27

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Abstract

The invention discloses a zearalenone lactone hydrolase mutant S162P with improved thermal stability and application thereof, and belongs to the technical field of enzyme engineering. According to the invention, zearalenone lactone hydrolase derived from a microorganism GliocladiumroseumMA918 is adopted as a parent, and a gene mutation technology is adopted to mutate serine (S) at the 162 site into proline (P) so as to obtain the single-site mutant S162P. Compared with a wild enzyme, on the basis that the original catalytic activity is not obviously changed, the thermal stability of the mutant enzyme is obviously improved: after the mutant enzyme is kept at 50 DEG C for 2 minutes, the residual enzyme activity is improved to be 1.52 times of that of the wild enzyme; and after heat preservation is conducted for 10 min and 20 min at the temperature of 48 DEG C respectively, about 80% and 58% residual enzyme activities are still remained, and the half-life period at the temperature of 48 DEG C is also increased by 3.6 times that of the wild enzyme. The invention lays a certain foundation for industrial application of the ZEN lactone hydrolase.
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Description

technical field

[0001] The invention relates to mutant S162P of zearalenone hydrolase with improved thermal stability and application thereof, belonging to the technical field of enzyme engineering. Background technique

[0002] Zearalenone (Zearalenone, ZEN) is a non-steroidal estrogen mycotoxin, the chemical name is 6-(10-hydroxy-6oxy-undecenyl) β-resorcinolactone . In 1962, ZEN was first discovered in moldy corn. It is a toxic secondary metabolite produced by Fusarium. It widely exists in moldy corn, wheat, barley and other grains and grain by-products. a worldwide food and feed contaminant. Usually, unsuitable storage conditions (10-30°C temperature and 40-50% ambient relative humidity) are the main cause of ZEN. In addition, ZEN also has a variety of toxic derivatives such as: α / β-Zearalenol (α / β-Zearalenol, α / β-ZOL), α / β-Zearalanol (α / β-Zearalanol , α / β-ZAL), zearalenone (Zearalanone, ZAN), etc. As an exogenous estrogen analogue, ZEN and its derivatives can compet...

Claims

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