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Method for synthesizing dihydromyricetin-7-glucoside through biological catalysis

A technology of dihydromyricetin and glucoside, which is applied in the field of bioengineering, can solve the problems of unfavorable industrial production, low water solubility, and restriction of research and development, and achieve the effects of repeated use, environmental friendliness, and good reaction selectivity

Pending Publication Date: 2021-08-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although dihydromyricetin has good solubility in hot water, the problem of low water solubility at room temperature limits the research and development of it.
Hye-Jin Woo et al. catalyzed the synthesis of dihydromyricetin-4'-O-α-D-glucose using the glucan transferase produced by Enterococcus enterococcus B-1299CB4, but further synthesis and purification of glucan was required Transferase, may be unfavorable for industrial production

Method used

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  • Method for synthesizing dihydromyricetin-7-glucoside through biological catalysis
  • Method for synthesizing dihydromyricetin-7-glucoside through biological catalysis
  • Method for synthesizing dihydromyricetin-7-glucoside through biological catalysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A method for biocatalytically synthesizing dihydromyricetin-7-glucoside, comprising the steps of:

[0066] (1) Preparation of induction medium containing carbohydrates and induction medium containing phenolic glycosides

[0067] Add sugar (select sucrose) in the induction medium, mix evenly, obtain the induction medium containing carbohydrate, the induction medium containing carbohydrate comprises: 5.0g / L sucrose, 5.0g / L Magnesium sulfate, the yeast extract of 10.0g / L, the disodium hydrogen phosphate of 5.0g / L, the potassium dihydrogen phosphate of 5.0g / L, the anhydrous calcium chloride of 5.0g / L, the remainder is water, described The pH of the induction medium containing carbohydrates is 6.0;

[0068] Add the phenolic compound (dihydromyricetin for use) into the induction medium and mix evenly to obtain the induction medium containing the phenolic compound. The induction medium containing the phenolic compound comprises: 15.0 dihydromyricetin element, 5.0g / L magnesiu...

Embodiment 2

[0078] A method for biocatalytically synthesizing dihydromyricetin-7-glucoside, comprising the steps of:

[0079] (1) Preparation of induction medium containing carbohydrates and induction medium containing phenolic glycosides

[0080] Add carbohydrates (glucose for use) into the induction medium and mix evenly to obtain an induction medium containing carbohydrates. The induction medium containing carbohydrates includes: 10.0 g / L of glucose, 5.0 g / L of Magnesium sulfate, the yeast extract of 10.0g / L, the disodium hydrogen phosphate of 5.0g / L, the potassium dihydrogen phosphate of 5.0g / L, the anhydrous calcium chloride of 5.0g / L, the remainder is water, described The pH of the induction medium containing carbohydrates is 7.4;

[0081] Add the phenolic glycoside compound (selecting dihydromyricetin) into the induction medium, and mix evenly to obtain an induction medium containing the phenolic glycoside compound, which includes: 10.0 g / L of Dihydromyricetin, 5.0g / L magnesium s...

Embodiment 3

[0090] A method for biocatalytically synthesizing dihydromyricetin-7-glucoside, comprising the steps of:

[0091] (1) Preparation of induction medium containing carbohydrates and induction medium containing phenolic glycosides

[0092] Add sugar (select fructose) in the induction medium, mix uniformly, obtain the induction medium containing the carbohydrate substance, the induction medium containing the carbohydrate substance comprises: 5.0g / L fructose, 2.5g / L Magnesium sulfate, yeast extract of 25.0g / L, disodium hydrogen phosphate of 25.0g / L, potassium dihydrogen phosphate of 2.5g / L, anhydrous calcium chloride of 2.5g / L, the remainder is water, described The pH of the induction medium containing carbohydrates is 6.0;

[0093] Add the phenolic compound (myricetin for use) into the induction medium, and mix evenly to obtain an induction medium containing the phenolic compound. The induction medium containing the phenolic compound comprises: 5.0 g / L of myricetin , 2.5g / L of ma...

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Abstract

The invention discloses a method for synthesizing dihydromyricetin-7-glucoside by virtue of biological catalysis. The method comprises the following steps: inoculating fungi into an induction culture medium containing carbohydrates, carrying out primary culture, conducting centrifuging to take precipitates to obtain fungi subjected to primary culture, inoculating the fungi into an induction culture medium containing phenolic glycoside compounds, carrying out secondary culture, and conducting centrifuging to take precipitates to obtain the whole-cell catalyst; and adding the whole-cell catalyst into a buffer solution mixed solution containing dihydromyricetin and starch, and conducting glycosylation reaction to generate the dihydromyricetin-7-glucoside. According to the method, the dihydromyricetin glucoside derivative can be efficiently synthesized with high selectivity, the problems that at present, glycosyltransferase is few in variety, high in price, difficult to react and the like are solved, and the method is easy to operate, low in cost and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a method for biocatalytically synthesizing dihydromyricetin-7-glucoside. Background technique [0002] Dihydromyricetin is a natural flavonoid compound, which is mostly extracted from vine tea plants of the genus Vitis vinifera. Dihydromyricetin has the functions of anti-oxidation, scavenging free radicals, anti-inflammation, antibacterial, lowering blood fat and protecting liver. Although dihydromyricetin has good solubility in hot water, the problem of low water solubility at room temperature limits its research and development. At 25°C, the solubility in water is only 0.67mM. At the same time, because dihydromyricetin is a polyphenolic hydroxyl compound with poor stability, it is easy to oxidize and decompose during application, especially when exposed to light, and form colorful photolysis products, which affects the performance of efficacy. [0003] At present, there are var...

Claims

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Application Information

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IPC IPC(8): C12P19/60C12N1/14C12R1/685C12R1/69C12R1/845C12R1/865C12R1/72
CPCC12P19/60C12N1/14Y02P20/584Y02A50/30
Inventor 李晓凤莫岚余以刚赵光磊吴晖
Owner SOUTH CHINA UNIV OF TECH