Preparation method of nanoparticle composition delivery system

A delivery system and nanoparticle technology, which can be used in drug combinations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of difficult to control the ratio of drug and gene load, and complex assembly process.

Inactive Publication Date: 2021-09-10
兰溪市立顺生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These strategies can load drugs and genes into the same nano-delivery system, which has a good synergistic therapeutic effect of drugs and genes, but there are many shortcomings, such as complex assembly process, use of organic solvents, and difficult to control the loading ratio of drugs and genes, etc.

Method used

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  • Preparation method of nanoparticle composition delivery system
  • Preparation method of nanoparticle composition delivery system
  • Preparation method of nanoparticle composition delivery system

Examples

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Embodiment 1

[0052] A method of preparing a nanoparticle composition delivery system:

[0053] Cationic liposomes of antitumor drugs were prepared by thin film dispersion method: 2-dioleoyl hydroxypropyl-3-N,N,N-trimethylammonium chloride and phytosterols with a molar ratio of 1:0.8:0.2:2.8-3.2 , diacetone-D-galactose and dioleoyl phosphatidylethanol, paclitaxel with a mass ratio of 1:6 to dioleoyl phosphatidylethanolamine, alkyl glycosides with a hydrophilic-hydrophobic balance of 4.5 and guanidinoacetic acid , dissolved in absolute ethanol, put the solution into an eggplant-shaped bottle, and rotate it under reduced pressure in a water bath to form a uniformly distributed lipid film, remove the residual solvent, add RNase-free water, hydrate, and squeeze out.

[0054]GAPDH siRNA-paclitaxel liposomes were prepared by the mixed incubation method: Dissolve Cy3-labeled GAPDH-siRNA in RNase-free water, mix cationic liposomes and Cy3-labeled GAPDH-siRNA at a mass ratio of 18:1, and let stand a...

Embodiment 2

[0056] A method of preparing a nanoparticle composition delivery system:

[0057] Antitumor drug cationic liposomes were prepared by thin film dispersion method: 2-dioleoyl hydroxypropyl-3-N,N,N-trimethylammonium chloride, phytosterol, bis Acetone-D-galactose and dioleoyl phosphatidylethanol, paclitaxel with a mass ratio of dioleoyl phosphatidylethanolamine of 1:6.6, alkyl glycosides with a hydrophilic-hydrophobic balance of 4.8 and guanidinoacetic acid, dissolved In absolute ethanol, put the solution into an eggplant-shaped bottle, and rotate it under reduced pressure in a water bath to form a uniformly distributed lipid film, remove the residual solvent, add RNase-free water, hydrate, and squeeze out.

[0058] Prepare GAPDH siRNA-paclitaxel liposomes by mixed incubation method: Dissolve Cy3-labeled GAPDH-siRNA in RNase-free water, mix cationic liposomes and Cy3-labeled GAPDH-siRNA at a mass ratio of 19.3:1, and let stand at room temperature. Cy3-labeled GAPDH siRNA-paclitax...

Embodiment 3

[0060] A method of preparing a nanoparticle composition delivery system:

[0061] Cationic liposomes of antitumor drugs were prepared by film dispersion method: 2-dioleoyl hydroxypropyl-3-N,N,N-trimethylammonium chloride, phytosterols, bis Acetone-D-galactose and dioleoyl phosphatidylethanol, paclitaxel with a mass ratio of dioleoyl phosphatidylethanolamine of 1:7, alkyl glycosides with a hydrophilic-hydrophobic balance of 5.2 and guanidinoacetic acid, dissolved In absolute ethanol, put the solution into an eggplant-shaped bottle, and rotate it under reduced pressure in a water bath to form a uniformly distributed lipid film, remove the residual solvent, add RNase-free water, hydrate, and squeeze out.

[0062] GAPDH siRNA-paclitaxel liposomes were prepared by the mixed incubation method: Dissolve Cy3-labeled GAPDH-siRNA in RNase-free water, mix cationic liposomes and Cy3-labeled GAPDH-siRNA at a mass ratio of 22:1, and let stand at room temperature. Cy3-labeled GAPDH siRNA-pa...

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Abstract

The invention provides a preparation method of a nanoparticle composition delivery system, and belongs to the field of nano biomaterials. The preparation method comprises the following steps: preparing a cationic liposome raw material and an antitumor drug into an antitumor drug-loaded cationic liposome by adopting a film dispersion method; and mixing the antitumor drug-loaded cationic liposome with GAPDH-siRNA by adopting a mixed incubation method to prepare the nanoparticle composition delivery system, wherein the cationic liposome raw material comprises 2-dioleoyl hydroxypropyl-3-N, N, N-trimethylamine chloride, phytosterol, diacetone-D-galactose and dioleoyl phosphatidyl ethanolamine. The delivery system prepared by the preparation method of the nanoparticle composition delivery system provided by the invention has the advantages of high encapsulation efficiency, good stability, large drug and gene loading capacity, small particle size change, high cell uptake efficiency and uptake rate and the like.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of May 22, 2019, an application number of 201910428250.8, and an invention title of "A nanoparticle composition delivery system, preparation method and use thereof". technical field [0002] The invention belongs to the field of nanobiological materials, and relates to a preparation method of a nanoparticle composition delivery system. Background technique [0003] The mechanism of tumorigenesis is very complex, usually due to the abnormal activation of some proto-oncogenes, the inactivation of tumor suppressor genes or apoptosis-related genes, resulting in the disorder of malignant cell proliferation and apoptosis. Difficult to make a breakthrough. [0004] The combined use of chemical drugs and therapeutic genes with different mechanisms of action can not only synergistically improve the therapeutic effect, but also increase the genetic barriers that cancer cells ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/54A61K9/127A61K47/26A61K47/28A61K31/7088A61K31/337A61P35/00
CPCA61K9/127A61K47/28A61K47/26A61K31/337A61P35/00A61K47/18A61K47/24A61K31/7105A61K2300/00
Inventor 不公告发明人
Owner 兰溪市立顺生物有限公司
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