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Aspergillus oryzae ZA175 and application thereof

A kind of Aspergillus oryzae, application technology, applied in the direction of fungi, microorganisms, and methods based on microorganisms, etc., can solve the problems of low production rate of soy sauce products, increase of soy sauce production cost, and increase the difficulty of quality control of soy sauce brewing process, etc.

Active Publication Date: 2021-09-10
FOSHAN HAITIAN GAOMING FLAVORING & FOOD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high concentration of moromi xylose fermented by this strain, the gradient of the color rate changes with the fermentation time is large, melanin is easily produced during the fermentation and sterilization of soy sauce, and the production rate of ammonia nitrogen and total nitrogen in soy sauce products is low. , leading to a further reduction in the deliciousness of soy sauce
[0007] At present, the main method is to add combined enzyme preparations in the soy sauce fermentation system or improve the production process to achieve the purpose of simultaneously improving the color and flavor quality of soy sauce products. On the one hand, it increases the difficulty of quality control in the soy sauce brewing process, and on the other hand, it increases the production cost of soy sauce.

Method used

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  • Aspergillus oryzae ZA175 and application thereof
  • Aspergillus oryzae ZA175 and application thereof
  • Aspergillus oryzae ZA175 and application thereof

Examples

Experimental program
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preparation example Construction

[0055] The present invention also provides a kind of preparation method of soy sauce or / and sauce, and described preparation method comprises the steps:

[0056] (1) aspergillus oryzae (Aspergillus oryzae) ZA175 described in claim 1 is inoculated to koji-making medium, cultivates;

[0057] (2) The koji prepared in step (1) is mixed with sodium chloride solution and fermented.

[0058] In one example, the koji-making medium contains bean raw materials or / and wheat raw materials. The bean raw materials mentioned in the present invention mainly refer to the raw materials derived from leguminous crops, such as soybean meal, which can be degreased beans or non-defatted beans. Beans include but are not limited to soybeans and soybeans. black beans. The wheat-based raw material described in the present invention can be wheat starch or bran. Preferably, the koji-making culture medium of the present invention comprises soybean, bran and flour.

[0059] In the process of preparing k...

Embodiment 1

[0067] Embodiment 1: the acquisition of Aspergillus oryzae (Aspergillus oryzae) ZA175

[0068] 1. Strain mutagenesis

[0069] The spores of the cultured mature starting strain Huyao 3.042 are made into a spore suspension, and the preparation method of the spore suspension is as follows:

[0070] Inoculate the activated Huyao 3.042 strain into PDA liquid medium, culture at 28°C, cultivate for 18h-24h to the logarithmic phase, then adjust the concentration of the strain suspension in the logarithmic phase with 0.8% normal saline, and pass through optical Microscope counted, the bacterial concentration was adjusted to 1 × 10 6 cfu / mL~1×10 8 cfu / mL. The concentration of the spore suspension of Huyao 3.042 used in this embodiment is 2×10 6 cfu / mL.

[0071] Take 95 μL of the above spore suspension, add 5 μL of 50% glycerol and mix well. ARTP mutagenesis was performed on spore suspensions. The ARTP mutagenesis conditions are as follows: Helium is used as the working gas, the d...

Embodiment 2

[0112] Embodiment 2: detection of genetic stability of Aspergillus oryzae (Aspergillus oryzae) ZA175 strain

[0113] The subculture stability of enzyme-producing strains obtained by mutagenesis is an important indicator related to the quality of strains. The mutagenized strain Aspergillus oryzae (Aspergillus oryzae) ZA175 obtained by breeding was continuously passaged on the slant of soybean juice medium for 10 generations, and the growth of each generation was observed. According to the method described in Example 1, the method is expanded step by step to prepare triangular bottle koji and koji, and its genetic stability is judged. If ten generations are used, the number of spores, neutral protease activity, amylase activity, and α-L-arabinosidase activity are measured. If the error of α-glucuronidase activity and α-glucuronidase activity is within 10%, it indicates that the genetic stability of the strain is good. See Table 5 for specific data.

[0114] Table 5. Koji-makin...

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Abstract

The invention relates to aspergillus oryzae ZA175 and application thereof. The aspergillus oryzae Z175 has the advantages of high dissolution rate of soybean protein and saccharides, high utilization rate of raw materials, high comprehensive enzyme activity and the like, has stable hereditary properties, is applied to the production process of soy sauce / sauce, does not need to additionally regulate and control koji making and fermentation processes, the aspergillus oryzae ZA175 can generate relatively high alpha-L-arabinofuranosidase and alpha-glucuronidase activity, the utilization rate of raw materials is high, and the energy consumption is low; and in the fermentation process, materials with various purposes do not need to be additionally added, the soy sauce / sauce which is fresh, sweet and prominent in flavor, mellow in taste, strong in sauce fragrance and lasting in mouth retention can be obtained, and industrial production of the additive-free purely-brewed soy sauce / sauce can be easily achieved. The finished product can be manufactured without blending other additives in the later period, the process steps are simple, and the production cost is saved; and when the aspergillus oryzae strain is used for starter propagation and fermentation of the soy sauce / sauce, the flavor, the color and the oxidation resistance of the soy sauce / sauce can be remarkably improved, and the product quality is improved.

Description

technical field [0001] The invention belongs to the technical field of brewing microorganisms, and in particular relates to a strain of Aspergillus oryzae and its application in soy sauce brewing. Background technique [0002] Soy sauce is made from soybeans, wheat flour, etc., and is fermented by microorganisms and enzymes. Aspergillus oryzae is the main strain of soy sauce fermentation, rich in intracellular enzymes, capable of degrading plant protein and polysaccharides to produce soy sauce. In general, the more abundant the enzyme system in Aspergillus oryzae, the higher the utilization rate of raw materials, the less precipitation produced in the production of soy sauce, and the richer the flavor of the finished product. [0003] During the soy sauce fermentation process, Aspergillus oryzae 3.042 has excellent characteristics such as the ability to secrete highly active hydrolytic enzymes and rapid growth, so it has become the most important industrial strain in the do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L27/50A23L27/60A23L11/50A23L29/00C12R1/69
CPCA23L27/50A23L27/60A23L11/50A23L29/065
Inventor 黄壮壮童星杨勇王寒童火艳张新峰
Owner FOSHAN HAITIAN GAOMING FLAVORING & FOOD
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