Aspergillus oryzae ZA175 and application thereof
A kind of Aspergillus oryzae, application technology, applied in the direction of fungi, microorganisms, and methods based on microorganisms, etc., can solve the problems of low production rate of soy sauce products, increase of soy sauce production cost, and increase the difficulty of quality control of soy sauce brewing process, etc.
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[0055] The present invention also provides a kind of preparation method of soy sauce or / and sauce, and described preparation method comprises the steps:
[0056] (1) aspergillus oryzae (Aspergillus oryzae) ZA175 described in claim 1 is inoculated to koji-making medium, cultivates;
[0057] (2) The koji prepared in step (1) is mixed with sodium chloride solution and fermented.
[0058] In one example, the koji-making medium contains bean raw materials or / and wheat raw materials. The bean raw materials mentioned in the present invention mainly refer to the raw materials derived from leguminous crops, such as soybean meal, which can be degreased beans or non-defatted beans. Beans include but are not limited to soybeans and soybeans. black beans. The wheat-based raw material described in the present invention can be wheat starch or bran. Preferably, the koji-making culture medium of the present invention comprises soybean, bran and flour.
[0059] In the process of preparing k...
Embodiment 1
[0067] Embodiment 1: the acquisition of Aspergillus oryzae (Aspergillus oryzae) ZA175
[0068] 1. Strain mutagenesis
[0069] The spores of the cultured mature starting strain Huyao 3.042 are made into a spore suspension, and the preparation method of the spore suspension is as follows:
[0070] Inoculate the activated Huyao 3.042 strain into PDA liquid medium, culture at 28°C, cultivate for 18h-24h to the logarithmic phase, then adjust the concentration of the strain suspension in the logarithmic phase with 0.8% normal saline, and pass through optical Microscope counted, the bacterial concentration was adjusted to 1 × 10 6 cfu / mL~1×10 8 cfu / mL. The concentration of the spore suspension of Huyao 3.042 used in this embodiment is 2×10 6 cfu / mL.
[0071] Take 95 μL of the above spore suspension, add 5 μL of 50% glycerol and mix well. ARTP mutagenesis was performed on spore suspensions. The ARTP mutagenesis conditions are as follows: Helium is used as the working gas, the d...
Embodiment 2
[0112] Embodiment 2: detection of genetic stability of Aspergillus oryzae (Aspergillus oryzae) ZA175 strain
[0113] The subculture stability of enzyme-producing strains obtained by mutagenesis is an important indicator related to the quality of strains. The mutagenized strain Aspergillus oryzae (Aspergillus oryzae) ZA175 obtained by breeding was continuously passaged on the slant of soybean juice medium for 10 generations, and the growth of each generation was observed. According to the method described in Example 1, the method is expanded step by step to prepare triangular bottle koji and koji, and its genetic stability is judged. If ten generations are used, the number of spores, neutral protease activity, amylase activity, and α-L-arabinosidase activity are measured. If the error of α-glucuronidase activity and α-glucuronidase activity is within 10%, it indicates that the genetic stability of the strain is good. See Table 5 for specific data.
[0114] Table 5. Koji-makin...
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