IPMA antibody detection method of Lawsonia intracellularis

A technology for antibody detection and Lawsonia, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of not being able to reflect the true state of pig herd infection, and being unable to distinguish pig serum, etc., to achieve simple, intuitive and effective detection methods, and reduce detection Cost, specific effect
CN113376375APending Publication Date: 2021-09-10NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2021-09-10

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Abstract

The invention discloses an IPMA antibody detection method of Lawsonia intracellularis. The method comprises the following steps: (1) culturing the Lawsonia intracellularis by using McCoy cells; (2) preparing an IPMA reaction plate: adding the Lawsonia intracellularis cultured in the step (1) into a cell plate containing McCoy cells for culturing, continuously culturing for 4-5 days, and then fixing, permeating and sealing in sequence; (3) adding to-be-detected serum into the IPMA reaction plate obtained in the step (2), respectively adding positive control serum and negative control serum at the same time, and incubating; adding a peroxidase-labeled secondary antibody, and incubating; and adding a developing solution, reacting and drying; and (4) observing under a microscope, and when the to-be-detected serum sample is positive of a lawsonia intracellularis antibody, staining the cytoplasm of the positive cell infected by the lawsonia intracellularis to be brownish red; and when a to-be-detected pig serum sample is negative in the lawsonia intracellularis antibody, enabling the cytoplasm of the McCoy cells infected by the lawsonia intracellularis to be not colored. The method has the advantages of high specificity, high sensitivity, simplicity in operation and the like.
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Description

technical field

[0001] The invention belongs to the technical field of Lawsonia intracellulare antibody detection, and in particular relates to an IPMA antibody detection method of Lawsonia intracellulare. Background technique

[0002] Porcine proliferative enteropathy (PPE), commonly known as porcine ileitis, is an important pig disease caused by Lawsonia intracellularis (LI) infection, characterized by thickening of the ileum, cecum, and colon mucosa. Intestinal disease. Infected epithelial cells generally cannot develop into mature intestinal epithelial cells. As time goes by, mature intestinal epithelial cells are replaced by infected immature cells after aging, resulting in the infected animals not having normal absorption function, resulting in disease. The growth and development of pigs slowed down, the feed conversion rate decreased, and the cost of breeding increased, causing farmers to suffer serious economic losses. According to reports, in the United States, th...

Claims

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