Application of multifunctional bacillus coagulans in probiotic nut production

A technology of Bacillus coagulans and probiotics, which is applied in the field of microbial application and food processing, can solve the problems of unpublished Bacillus coagulans strains, application effect verification, etc., and achieve the effects of inhibiting the growth of pathogenic bacteria, high product safety and low hygroscopicity

Active Publication Date: 2021-09-17
CHACHA FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, CN110521988A does not disclose the Bacillus coagulans strains

Method used

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  • Application of multifunctional bacillus coagulans in probiotic nut production
  • Application of multifunctional bacillus coagulans in probiotic nut production
  • Application of multifunctional bacillus coagulans in probiotic nut production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Screening and performance measurement of embodiment 1 bacillus coagulans

[0054] 1 Screening, identification and storage of strains

[0055] (1) First crush the pickled cabbage, add 15mL sterile water, shake vigorously for 20min, then act in a water bath at 80°C for 20min; The formula is: yeast extract 10.0g / L; beef extract 10.0g / L; peptone 10.0g / L; sodium bicarbonate 1.5g / L; potassium dihydrogen phosphate 1.5g / L; magnesium sulfate heptahydrate 1.0g / L; sulfuric acid Manganese 0.1g / L; calcium chloride 1.0g / L; deionized water 1L), cultured at 45°C for 48h, and selected strains with large colonies and fast growth were further separated and purified by streaking.

[0056] (2) Preserve the isolated and purified bacterial strain obtained in the previous step in a glycerol tube; then take the bacterial liquid and inoculate it in the MRS medium, and cultivate it until the cell concentration is 10 8 cfu / mL, then according to the final concentration of 10 7 The added amount o...

Embodiment 2

[0107] Example 2 Application of multifunctional Bacillus coagulans BC2000 in the production of probiotic nuts

[0108] The method of applying multifunctional Bacillus coagulans BC2000 to the production of probiotic nuts is as follows:

[0109] (1) Preparation of probiotic coating powder:

[0110] Weigh 5 parts of Bacillus coagulans (spore content up to 1×10 9 More than cfu / g, according to the number of parts by mass, the same below) is pre-mixed with 350 parts of glucose powder to ensure that the two are evenly mixed, and then mixed with 350 parts of fermented yogurt powder, 11 parts of malic acid, 16 parts of milk mineral salt, 55 parts Fructose-oligosaccharides and 3 parts of Haematococcus pluvialis are put into a three-dimensional mixer for thorough mixing;

[0111] (2) Mucus preparation:

[0112] Use 5 parts of isomalt, 30 parts of maltodextrin, and 90 parts of water to make a coating solution;

[0113] (3) Probiotic Nut Wrap

[0114] Put 10 parts of peeled walnut ker...

Embodiment 3

[0120] (1) Preparation of probiotic coating powder:

[0121] Weigh 2 parts of Bacillus coagulans and 350 parts of glucose powder for pre-mixing to ensure that the two are evenly mixed, and then mix with 350 parts of fermented yogurt powder, 11 parts of malic acid, 16 parts of milk mineral salt, 55 parts of fructooligosaccharides, and 3 parts of rain Raw Haematococcus are put into the three-dimensional mixer together for thorough mixing;

[0122] (2) Mucus preparation:

[0123] Use 5 parts of isomalt, 30 parts of maltodextrin, and 90 parts of water to make a coating solution;

[0124] (3) Probiotic Nut Wrap

[0125] Put 10 parts of peeled walnut kernels into the coating equipment, and put the mucus into the liquid storage tank at the mucus adding end of the coating equipment. In the powder storage tank, the coating powder addition parameters are: 1 part of coating powder, time 1min, rotation speed set to 40Hz, start the equipment. After 1 minute, the nuts with mucus in the ...

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Abstract

The invention discloses application of multifunctional bacillus coagulans in probiotic nut production, and belongs to the technical field of microorganism application and the technical field of food processing. The bacillus coagulans has various excellent properties, and is used for probiotic nut production, so that probiotic nuts are endowed with better quality and higher safety. According to a probiotic nut production process, probiotic surface coating powder produced by the process is uniform and attractive, is not easy to fall off and does not contaminate hands when being eaten. The coating powder on the surfaces of the probiotic nuts forms a protective layer, so that oxygen can be prevented from being in contact with the surfaces of the nuts; and meanwhile, the coating powder cooperates with haematococcus pluvialis in the probiotic nuts, nitrogen-filled packages and oxygen absorbents, so that the oxidation rancidity of the nuts can be delayed, the oxidation rancidity of the surfaces of the nuts can be reduced, and the quality of the probiotic nuts can be guaranteed.

Description

technical field [0001] The invention relates to the application of multifunctional bacillus coagulans in the production of probiotic nuts, and belongs to the technical field of microbe application and food processing. Background technique [0002] Nuts are a category of closed fruits that have a hard skin and contain one or more seeds. Fruits such as walnuts and cashews are the essence of plants. Nuts contain 36.0% protein, 58.8% fat, and 72.6% carbohydrates, as well as vitamins (vitamin B, vitamin E, etc.), trace elements (phosphorus, calcium, zinc, iron), dietary fiber, etc. In addition, it also contains monounsaturated fatty acids and polyunsaturated fatty acids, including essential fatty acids such as linolenic acid and linoleic acid. It has excellent effects on human growth and development, strengthening physical fitness and preventing diseases. According to research by an authoritative organization, eating nuts more than twice a week can reduce people's risk of fata...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L25/00A23L33/135A23L33/21A23L33/10A23B9/28C12R1/07
CPCA23L25/40A23L25/25A23L33/135A23L33/21A23L33/10A23B9/28A23V2002/00A23V2200/22A23V2200/02A23V2200/3204A23V2200/3202A23V2200/324A23V2250/61A23V2250/1644A23V2250/044A23V2250/284A23V2250/6412A23V2250/5114A23V2300/10Y02A40/90
Inventor 倪伟超金龙张飞卢肖蒙郑洁黄彩虹
Owner CHACHA FOOD CO LTD
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