A strain of Bacillus amyloliquefaciens and its role in disease resistance and growth promotion of Pinellia chinensis
A technology for dissolving starch spores and promoting growth agent, which is applied in the fields of plant growth regulators, microorganism-based methods, chemicals for biological control, etc.
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Embodiment 1
[0032] Example 1: Screening of Pinelliae disease-resistant functional strains
[0033] The 32 strains of bacteria preserved in the laboratory (including 14 strains of Bacillus subtilis, 6 strains of Bacillus licheniformis, 3 strains of Bacillus amyloliquefaciens, 1 strain of Bacillus megafilus, 1 strain of Veles) were tested by the filter paper method. Bacillus velezensis, 1 strain of Paenibacillus pabuli, 1 strain of Paenibacillus taichungensis, 1 strain of Bacillus cereus, 1 strain of Bacillus sonorensis, 1 strain of Cellulosimicrobium funkei, 1 strain of Rhodococcus hoagii, and 1 strain of Saccharomyces cerevisiae) for preliminary screening of 6 strains of antagonistic bacteria of Pinellia ternata, and the 6 strains of pathogenic bacteria were Fusarium solani, Fusarium oxysporum, and Alternaria Aspergillus, Phytophthora capsici, Fusarium, Pectobacterium carotovorum subsp.carotovorum (Jones) Hauben et, have a transparent circle, indicating that there is an antibacterial effe...
Embodiment 2
[0046] Antagonism of Bacillus amyloliquefaciens 8-2 against the pathogenic bacteria of Pinellia chinensis
[0047] Bacteriostatic test by double plate culture method
[0048] Take the petri dish of Fusarium solani and Alternaria solani, punch out a 5mm diameter bacteria cake at the edge, and place it on one side of the PDA plate. Antagonistic bacteria were inoculated at a distance of 4 cm from the fungus cake. The plates only inoculated with Fusarium solani and Alternaria were used as controls. Place them in a constant temperature incubator at 25°C for cultivation, measure the radius of the control colony after 7 days, and calculate the bacteriostatic rate.
[0049] Bacterial inhibition rate = (B-A) / Bx100%
[0050] A: Pathogen colony diameter of the treatment group of inoculated bacteria, cm
[0051] B: Pathogen colony diameter of the control group, cm
[0052] The antibacterial rate of the strain was calculated by the double-plate culture method, and the higher the antib...
Embodiment 3
[0056] Phosphorus-solubilizing effect of Bacillus amyloliquefaciens 8-2:
[0057] The phosphorus-solubilizing activity of the strain can be preliminarily judged by the ratio of the colony + phosphorus-solubilizing circle to the colony diameter (D / d). Qualitative detection of inorganic phosphorus-solubilizing ability: inoculate the strains in inorganic phosphorus solid medium, and culture them upside down at 30°C for 7 days, then measure the diameter of the phosphorus-dissolving circle (D1) and the diameter of the colony (d1) and the ratio of D1 / d1, and the strains were repeated three times. Quantitative detection of the ability to decompose inorganic phosphorus: the bacterial strains screened out from the above experimental process were cultured in LB liquid medium for 24 hours (making the concentration 1×10 8 cfu / mL), 2% were inoculated in the inorganic phosphorus liquid medium, and the inorganic phosphorus liquid medium without bacteria was used as the control group. Cultiv...
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