A strain of Bacillus amyloliquefaciens and its role in disease resistance and growth promotion of Pinellia chinensis

A technology for dissolving starch spores and promoting growth agent, which is applied in the fields of plant growth regulators, microorganism-based methods, chemicals for biological control, etc.

Active Publication Date: 2022-05-20
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Tang Hanmeng (2019) studied the effects of different doses of three microbial agents (Bacillus coliformis F1, Bacillus amyloliquefaciens PC06 and Streptomyces jingyang 5406) ​​and biochar (600 °C carbonized chaff biochar) on the effects of Pinellia chinensis. The results showed that the application of Bacillus amyloliquefaciens F1 and Streptomyces 5406 could significantly improve the growth vigor, yield and quality of Pinellia pinellia, but the application of Bacillus amyloliquefaciens PC06 and No significant difference in control group

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  • A strain of Bacillus amyloliquefaciens and its role in disease resistance and growth promotion of Pinellia chinensis
  • A strain of Bacillus amyloliquefaciens and its role in disease resistance and growth promotion of Pinellia chinensis
  • A strain of Bacillus amyloliquefaciens and its role in disease resistance and growth promotion of Pinellia chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Screening of Pinelliae disease-resistant functional strains

[0033] The 32 strains of bacteria preserved in the laboratory (including 14 strains of Bacillus subtilis, 6 strains of Bacillus licheniformis, 3 strains of Bacillus amyloliquefaciens, 1 strain of Bacillus megafilus, 1 strain of Veles) were tested by the filter paper method. Bacillus velezensis, 1 strain of Paenibacillus pabuli, 1 strain of Paenibacillus taichungensis, 1 strain of Bacillus cereus, 1 strain of Bacillus sonorensis, 1 strain of Cellulosimicrobium funkei, 1 strain of Rhodococcus hoagii, and 1 strain of Saccharomyces cerevisiae) for preliminary screening of 6 strains of antagonistic bacteria of Pinellia ternata, and the 6 strains of pathogenic bacteria were Fusarium solani, Fusarium oxysporum, and Alternaria Aspergillus, Phytophthora capsici, Fusarium, Pectobacterium carotovorum subsp.carotovorum (Jones) Hauben et, have a transparent circle, indicating that there is an antibacterial effe...

Embodiment 2

[0046] Antagonism of Bacillus amyloliquefaciens 8-2 against the pathogenic bacteria of Pinellia chinensis

[0047] Bacteriostatic test by double plate culture method

[0048] Take the petri dish of Fusarium solani and Alternaria solani, punch out a 5mm diameter bacteria cake at the edge, and place it on one side of the PDA plate. Antagonistic bacteria were inoculated at a distance of 4 cm from the fungus cake. The plates only inoculated with Fusarium solani and Alternaria were used as controls. Place them in a constant temperature incubator at 25°C for cultivation, measure the radius of the control colony after 7 days, and calculate the bacteriostatic rate.

[0049] Bacterial inhibition rate = (B-A) / Bx100%

[0050] A: Pathogen colony diameter of the treatment group of inoculated bacteria, cm

[0051] B: Pathogen colony diameter of the control group, cm

[0052] The antibacterial rate of the strain was calculated by the double-plate culture method, and the higher the antib...

Embodiment 3

[0056] Phosphorus-solubilizing effect of Bacillus amyloliquefaciens 8-2:

[0057] The phosphorus-solubilizing activity of the strain can be preliminarily judged by the ratio of the colony + phosphorus-solubilizing circle to the colony diameter (D / d). Qualitative detection of inorganic phosphorus-solubilizing ability: inoculate the strains in inorganic phosphorus solid medium, and culture them upside down at 30°C for 7 days, then measure the diameter of the phosphorus-dissolving circle (D1) and the diameter of the colony (d1) and the ratio of D1 / d1, and the strains were repeated three times. Quantitative detection of the ability to decompose inorganic phosphorus: the bacterial strains screened out from the above experimental process were cultured in LB liquid medium for 24 hours (making the concentration 1×10 8 cfu / mL), 2% were inoculated in the inorganic phosphorus liquid medium, and the inorganic phosphorus liquid medium without bacteria was used as the control group. Cultiv...

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Abstract

The invention belongs to the field of microorganism application and discloses a bacillus amyloliquefaciens strain and its effect on disease resistance and growth promotion of pinellia pinellia. The preservation number of the bacillus amyloliquefaciens is: CCTCC NO: M2021822. The present invention provides for the first time a strain of Bacillus amyloliquefaciens 8‑2, which has a strong inhibitory effect on the causative agent of pinellia tuber rot, Fusarium solani and Alternaria alternaria. Phosphorus and the function of promoting the rapid growth and germination of Pinellia provide high-quality seed strains for the development of Pinellia microbial fertilizers, which have a good application prospect in the disease control and planting of Pinellia.

Description

technical field [0001] The invention belongs to the field of microorganism application, and in particular relates to a strain of bacillus amyloliquefaciens and its effect on disease resistance and growth promotion of pinellia pinellia. Background technique [0002] Pinellia is a perennial herbaceous plant belonging to the Araceae family Pinellia. Its dried tubers are one of the traditional Chinese medicinal materials in my country. Among the 558 kinds of traditional Chinese medicine prescriptions, pinellia ranks the 22nd most frequently used medicinal material. The wild resources of pinellia are depleted day by day, and the artificial planting of pinellia has become the most important source of medicinal materials, but the artificial planting has been plagued by continuous cropping obstacles and soil-borne diseases. Soil-borne diseases of pinellia have become an important factor to curb the development of pinellia artificiala industry. [0003] Pinellia tuber rot is the ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G7/06A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01G7/06A01N63/22Y02A40/10
Inventor 吴健张雨薇徐明江任竹青彭彦李展鑫
Owner HUAZHONG AGRI UNIV
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