Iron-reducing bacterium Tessaracoccus oleiagri DH10 strain and application of iron-reducing bacterium Tessaracoccus oleiagri DH10 strain
A strain, M2021404 technology, applied in the field of microbial oil recovery, can solve the problem of less iron-reducing bacteria, and achieve the effect of inhibiting clay swelling and improving oil recovery
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Embodiment 1
[0020] Example 1 Isolation and identification of iron-reducing bacteria
[0021] 1. Isolation of iron-reducing bacteria
[0022] The invention provides an iron-reducing bacterium isolated from the oil reservoir environment. The screening method is as follows: according to the conventional strain screening method, take 10 mL of water samples collected from the oil field, and insert them into 100 mL of Fe(OH) 3 (0.5%Fe(OH) 3 , w:v) in the anaerobic bottle, 35 ℃ static anaerobic culture for 1 week, select the experimental group whose solid phase color becomes darker, and in the anaerobic operation station, take 100 μL of fermentation broth under anaerobic conditions and spread it on LB agar plate medium, anaerobic culture at 35°C for 48 hours; single colonies of different shapes were picked, streaked and purified on LB agar plate medium, and cultured at 35°C for 48 hours. Pick a single colony and inoculate it into Fe(OH) through enrichment culture 3 In the culture medium, 35°C...
Embodiment 2
[0033] Embodiment 2 DH10 bacterial strain is to the decomposition of montmorillonite
[0034] 1. Strains
[0035] The Tessaracoccus oleiagri DH10 strain obtained by screening in Example 1.
[0036] 2. Medium
[0037] The composition of the medium is: sucrose 10-50g / L, sodium acetate 10-40g / L, sodium lactate 3-20g / L, MgSO4 0.1-2g / L, KCl 2-18g / L, MnSO4 5-9g / L, CuSO4 5-10g / L, ZnSO4 5-12g / L, KH2PO4 1-7g / L, montmorillonite 2-10g / L, pH 5.0-9.0. After the preparation of the medium, it was sterilized at 0.1MPa for 30min.
[0038] 3. Fermentation culture
[0039] Use an inoculation loop to mark the strain on the plate for activation, and culture it at 35°C for 20 hours, then pick three rings (each inoculation loop contains more than 3 single colonies with obvious characteristics) from the plate and transfer it to the shake flask (50mL Erlenmeyer flask, liquid volume is 30mL), cultivated at 35°C, 180r / min for 8h, collected the bacteria by centrifugation, washed with sterile water 3...
Embodiment 3
[0046] Example 3 Determination of anti-expansion rate
[0047] On the basis of Example 2, with reference to the determination method of clay anti-expansion rate in the standard clay stabilizer, the expansion properties of montmorillonite minerals before and after the action of Fe(III) reducing bacteria Tessaracoccus oleiagri DH10 strain were measured. The experimental steps are as follows: firstly weigh 0.1 g of the dried smectite sample of the control group and put it into a 2.0 mL centrifuge tube, record the volume V0 of the sample before it expands in the centrifuge tube; then add 1.5 mL of expansion medium solution ( Take distilled water and kerosene as the expansion medium solution) and shake well; place it at room temperature for 24 hours, put it in a centrifuge, and centrifuge at a speed of 5000 rpm for 15 minutes, and read the volume V1 of the sample after expansion. Repeat the above steps to measure the expansion volume of the montmorillonite samples in the experiment...
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