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Preparation method and application of strontium-doped modified natural hydroxyapatite scaffold material

A technology of hydroxyapatite and bovine bone hydroxyapatite, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of poor degradation performance and osteogenesis ability, unsatisfactory mechanical properties of scaffolds, and accelerated bone defect repair, etc. problem, to achieve the effect of improved solubility, simple preparation method, and accelerated repair

Pending Publication Date: 2021-10-22
北京市创伤骨科研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing scaffold materials for bone repair also generally have shortcomings such as rapid drug release, unsatisfactory mechanical properties of the scaffold, poor degradation performance and osteogenesis ability, etc. Bone repair scaffold materials that are strong, have strong mechanical properties and osteogenic ability, and can accelerate the repair of bone defects are of great significance in the field of bone repair therapy.

Method used

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  • Preparation method and application of strontium-doped modified natural hydroxyapatite scaffold material
  • Preparation method and application of strontium-doped modified natural hydroxyapatite scaffold material
  • Preparation method and application of strontium-doped modified natural hydroxyapatite scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1 Preparation of strontium-doped modified natural hydroxyapatite scaffold material

[0092] 1. Experimental materials

[0093] Bovine bone (cancellous bone) was purchased from Dachang Hui Autonomous Region Fuhua Meat Co., Ltd. (China); strontium chloride hexahydrate (analytical grade) was purchased from Aladdin Reagent (Shanghai) Co., Ltd. (China); Cell Staining Kit (Cat. No. C2015M) was purchased from Beyond (China); MSC FBS (Cat. No. 04-400-1A) was purchased from Biological Industries (Israel); L-DMEM (Cat. No. SH30021.01) was purchased from In Hyclone (USA); Crystal Violet Dye Solution (Cat. No. C0121) was purchased from Beyontien (China); CCK-8 (Cat. No. CK04) was purchased from Nippon Dojin Chemical (Japan).

[0094] 2. Preparation of calcined bovine bone hydroxyapatite scaffold material

[0095] (1) The source of cancellous bone: take the cancellous bone of the lower segment of femur of 3-year-old adult bovine, and cut out 30mm*20mm*15mm pieces;

[0096...

Embodiment 2

[0136] Embodiment 2 direct hemolysis test

[0137] 1. Experimental method

[0138] The direct hemolysis test is a method used in the national standard GB / T16886.4 for evaluating the biocompatibility of medical devices. Therefore, in this example, four groups of materials (0, 4.8%, 6.8%, 9.8% SrHAP) were directly hemolyzed experiment;

[0139]Weigh 0.2g of each of the 4 groups of sieved material particles and add them to a centrifuge tube containing 10mL of normal saline as the experimental group; the positive control of the experiment is deionized water; the negative control is normal saline; placed in a constant temperature shaker After incubating at 37°C for 30min, add 0.2mL of diluted rabbit blood (normal saline:rabbit blood=5:4), then put it in a constant temperature shaker at 37°C again, incubate for 60min, centrifuge at 2500r / min for 5min at room temperature, and take the supernatant Measure the absorbance at 545nm on a microplate reader, hemolysis rate (%)=(D sample-D...

Embodiment 3

[0144] Example 3 Cell adhesion and survival status on strontium-doped modified natural hydroxyapatite scaffold

[0145] 1. Cell culture

[0146] Human bone marrow-derived mesenchymal stem cells (hMSCs), donated by Zou Xuenong's research group at Sun Yat-sen University, are immortalized cells transfected with telomerase, which are not tumorigenic, but It can maintain the physiological characteristics of normal cells and has multi-directional differentiation potential. It is normally cultured in L-DMEM containing 10% FBS and 1% PS, subcultured for about 3-4 days, and the culture conditions are 37°C, 5% CO 2 ; The induction medium for the differentiation experiment, on the basis of ordinary medium, additionally add osteoinductive medium (comprising 10 mmol / L of β-sodium glycerophosphate, 10-7 mol / L of dexamethasone, and 50 μg / L of vitamin C).

[0147] 2. Cell adhesion experiment

[0148] The four groups of materials (0, 4.8%, 6.8%, 9.8% SrHAP) were cut into small squares with a...

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Abstract

The invention discloses a preparation method and application of a strontium-doped and modified natural hydroxyapatite scaffold material. The strontium-doped and modified natural hydroxyapatite scaffold material has excellent biocompatibility, biological activity, solubility and capability of promoting bone tissue regeneration. Strontium ions can be released in a continuous and slow mode, the released strontium ions can stimulate osteogenic differentiation, the function of osteoclast can be inhibited, bone defect repairing is accelerated, and the bone defect repairing agent has great clinical transformation application prospects in bone defect repairing treatment.

Description

technical field [0001] The invention belongs to the technical field of biomaterials. Specifically, the invention relates to a strontium-doped bovine bone-derived hydroxyapatite scaffold material. More specifically, the invention relates to a strontium-doped modified natural hydroxyapatite Preparation methods and applications of scaffold materials. Background technique [0002] Bone defect refers to a disease that damages the complete structure of bone due to trauma, inflammation, bone disease and other diseases or surgery. Bone is a metabolically active connective tissue with the ability to fully regenerate and remodel, but in the case of large bone defects, such as segmental injuries, tumor resection, the reconstruction process requires additional support. Clinically, autologous grafting remains the most common strategy to support and stimulate bone growth, and although ideal from a biological standpoint, the number of grafts provided by the patient's own body is limited a...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/36A61L27/02A61L27/18A61L27/24A61L27/20A61L27/22A61L27/16A61L27/50A61L27/54
CPCA61L27/12A61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/025A61L27/3616A61L27/18A61L27/24A61L27/20A61L27/222A61L27/16A61L27/50A61L27/54A61L2430/02A61L2300/412A61L2300/602A61L2300/102A61L2430/40
Inventor 陈大福万奔曹晶晶王宏刚郭建勋王芊黎广平王任先聂井君吴新宝蒋协远
Owner 北京市创伤骨科研究所
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