Composite microbial algae removal microbial agent and preparation method and application thereof

A technology of compound microorganisms and microbial strains, applied in the field of microbial engineering, can solve the problems of complex components of biological preparations, tedious preparation process, long time for algae removal, etc., and achieve the effect of increasing reuse channels, easy promotion and application, and less material use

Pending Publication Date: 2021-11-05
HUATIAN NANJING ENG & TECH CORP MCC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a compound microbial algae removal agent and its preparation method and application to solve the problems of long time for a single microorganism to remove algae, and multi-component biological preparations with complex components, cumbersome preparation process and high cost

Method used

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  • Composite microbial algae removal microbial agent and preparation method and application thereof
  • Composite microbial algae removal microbial agent and preparation method and application thereof
  • Composite microbial algae removal microbial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of a compound microbial algicide, comprising the following specific steps: respectively activating Z-1 algae-dissolving bacteria and XN-1 flocculating bacteria, which can be activated by scribing on LB plates; Bacteria and XN-1 flocculation bacteria were expanded to obtain seed liquid. Specifically, the activated Z-1 alginolytic bacteria and XN-1 flocculation bacteria were respectively inoculated into a 1L Erlenmeyer flask containing 500mL broth medium. Cultivate on a shaking table at 30-35°C, 100-300rpm / min for 12-24h, preferably at 30°C, 180rpm / min for 24h; take Z-1 algophilic bacteria and XN-1 The seed liquid of the flocculating bacteria is co-fermented and cultivated to obtain the bacterial agent.

Embodiment 2

[0032] The seed liquid of Z-1 alginolytic bacteria and XN-1 flocculating bacteria in the above example 1 were fermented and cultivated together according to the ratio of 1:1, 2:1, and 3:1 to obtain three bacterial agents, and the growth of the bacteria was tested. and algae-dissolving performance, in different inoculation ratios, the growth of the two strains can be seen figure 1 , there is no significant difference in the growth of the two strains, and they can be co-cultivated under the same conditions; at the same time, the fermentation broth obtained from different inoculation ratios is used to treat Microcystis aeruginosa, and the algicidal effect is tested. The results are shown in figure 2 , where the test uses Microcystis aeruginosa liquid, and the concentration of algae is 1×10 6 When cell / ml, the different inoculation ratios of each group are based on the same volume ratio of the bacterial agent and algae liquid, at a temperature of 20-35°C, the light intensity is 1...

Embodiment 3

[0038] High-density fermentation has higher requirements on the type and content of nutrient sources in the substrate, and the ratio of carbon sources to nitrogen sources will affect the growth of strains, thereby affecting the algae-dissolving effect;

[0039] Test the ratio of carbon source glucose and nitrogen source ammonium chloride in the fermentation medium, select three groups of ratios of 2:1, 1:1, and 1:2, under the conditions of aeration rate 1L / min, rotation speed 100rpm, temperature 30°C, After 3 days of fermentation, the algae-dissolving rate of the bacterial agent was tested, and the results are shown in image 3 , the carbon-nitrogen ratio is 1:1, the algae-dissolving effect is the best, and the preferred concentration is 0.5‰;

[0040] In a preferred embodiment, the fermentation medium can be prepared by the following method: introduce the tail water of the urban sewage plant into the fermentation culture device, add ammonium chloride at a mass ratio of 0.2‰, ...

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Abstract

The invention discloses a composite microbial algae removal microbial agent and a preparation method and application thereof, relates to the technical field of microbial engineering, and aims to solve the problems that the algae removal time of a single microorganism is long, and a multi-component biological agent is complex in component, tedious in preparation process and relatively high in cost. The composite microbial algae removal microbial agent comprises Z-1 algae-lysing bacteria and XN-1 flocculating bacteria. The preparation method comprises the following steps of: respectively activating the Z-1 algae-lysing bacteria and the XN-1 flocculating bacteria; respectively performing enlarged culture on the activated Z-1 algae-lysing bacteria and the XN-1 flocculating bacteria to obtain seed solutions; and performing co-fermentation culture on the seed solutions of the Z-1 algae-lysing bacteria and the XN-1 flocculating bacteria to obtain the microbial agent. The composite microbial algae removal microbial agent can be used for treating algae-polluted water, and the algae-lysing rate can reach 91.2% after 36 hours; the XN-1 flocculating bacteria and the Z-1 algae-lysing bacteria generate a relatively good synergistic effect, so that the algae removal rate is higher, and the efficiency is higher; and the composite microbial algae removal microbial agent is less in material use, only a small amount of strains are needed for self simple culture, the cost is relatively low, the market demand can be better met, and the popularization and application are easy.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a compound microbial algaecide and its preparation method and application. Background technique [0002] With the advancement of industry and the development of science and technology, various domestic sewage and urban wastewater are discharged into rivers, lakes and seas, resulting in eutrophication of water bodies, massive algal blooms, and outbreaks of algal blooms and red tides. The main hazards of algae are as follows: large areas of algae cover the water surface, causing water corruption and suffocation of fish; many blue-green algae can produce algal toxins that can cause damage to the nervous system, causing convulsions, breathing difficulties and even respiratory failure in humans or animals , may also cause diarrhea, vomiting, and damage to organs such as liver and kidney; affect environmental indicators such as water transparency and dissolved oxygen, lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/07
CPCC12N1/20C02F3/34
Inventor 崔倩倩费晓昕骆守鹏朱红生
Owner HUATIAN NANJING ENG & TECH CORP MCC
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