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Algal polysaccharide extract as well as preparation method and application thereof

A seaweed polysaccharide and extract technology, applied in the field of medicine and biology, can solve the problems of inability to remove the toxicity of sevage reagents, strict operating conditions and high equipment requirements, and achieve the effects of low cost, good removal effect and high purity

Active Publication Date: 2021-11-09
山东洁壹选生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Chinese patent application CN 104403018A discloses a method for extracting seaweed polysaccharides under high pressure, and patent application CN 04892785A discloses a method for extracting seaweed polysaccharides under ultra-high pressure. Both methods are carried out under high pressure and require high equipment , and the cost is high; patent application CN105580975A discloses a method for extracting seaweed protein by microbial fermentation, which requires microbial cultivation and strict and complicated operating conditions; patent application CN104341536A discloses a method for extracting seaweed protein A method for nutrients in seaweed, the method uses compound enzymes to pretreat seaweed to extract sodium alginate, and the cost is relatively high; patent application CN103951737A discloses a method for extracting glycoproteins from seaweed, which only extracts from seaweed Glycophilic protein; patent application CN103739726A discloses a method for extracting polysaccharides from seaweed, extracting polysaccharides under normal pressure, removing proteins by sevage method, and purifying polysaccharides with running water dialysis. Although this method is easy to operate, running water dialysis cannot remove small Molecular impurities are completely removed and the sevage reagent is highly toxic
[0009] However, obtaining high-purity seaweed polysaccharides from extracts containing seaweed polysaccharides usually requires purification and separation using chromatographic columns, which affects its application in industrial production

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  • Algal polysaccharide extract as well as preparation method and application thereof
  • Algal polysaccharide extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Using the method of the present invention to flocculate and extract the laminarin in the kelp, the steps are as follows:

[0036] (1) Pretreatment: Take 2 kg of dried kelp, crush it, add 2 times the amount (weight) of ethanol with a concentration of 95%, ultrasonicate for 1 hour, filter the soaking solution through a filter cloth, evaporate to dryness under reduced pressure at 80°C, remove Lipids, monosaccharides and other alcohol-soluble components contained in seaweed (alkaloids, flavonoid glycosides, polyphenols, amino acids, etc.);

[0037](2) Add the pretreated seaweed raw material to the potassium hydrogen phthalate solution with a pH of 4 as the extractant at a solid-to-liquid ratio of 1:5 (g / mL), filter after leaching, and depressurize the filtrate at 80°C Concentrate; then add a carbonate buffer solution with a pH of 9.6 according to a solid-to-liquid ratio of 1:5 (g / mL), filter after leaching, concentrate the filtrate under reduced pressure at 80°C, and desalt...

Embodiment 2

[0041] Using the method of the present invention to flocculate and extract the seaweed polysaccharide in the sargassum, the steps are as follows:

[0042] (1) Pretreatment: Take 2 kg of dried sargassum, crush it, add 2 times the amount (weight) of ethanol with a concentration of 85%, ultrasonicate for 1 hour, filter the soaking solution through filter cloth, and evaporate to dryness under reduced pressure at 80°C , remove lipids, monosaccharides and other alcohol-soluble components (alkaloids, flavonoid glycosides, polyphenols, amino acids, etc.) contained in seaweed;

[0043] (2) Add the pretreated seaweed raw material to the potassium hydrogen phthalate solution with a pH of 4 as the extractant at a solid-to-liquid ratio of 1:5 (g / mL), filter after leaching, and depressurize the filtrate at 80°C Concentrate; then add a carbonate buffer solution with a pH of 9.6 according to a solid-to-liquid ratio of 1:5 (g / mL), filter after leaching, concentrate the filtrate under reduced p...

Embodiment 3

[0050] Embodiment 3: antibacterial experiment and free radical scavenging experiment

[0051] Experiment 1: Antibacterial experiment of seaweed polysaccharide prepared by the present invention

[0052] The seaweed polysaccharide prepared above was dissolved in deionized water at a concentration of 5 wt % to obtain the seaweed polysaccharide antibacterial hand sanitizer prepared by the present invention. It is used for inhibition experiments of Escherichia coli, Staphylococcus aureus and Candida albicans.

[0053] experimental method:

[0054] 1) Doubling dilution method

[0055] Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923), and Candida albicans (ATCC90028) are configured to 0.5 McFarland turbidity with nutrient broth, and then diluted 1000 times for later use; Embodiment 1, Example 2, comparison The seaweed polysaccharide prepared in Example 1 and Comparative Example 2 was configured into a 50 mg / mL aqueous solution for later use; in a 96-well plate, anti...

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Abstract

The invention discloses an algal polysaccharide extract with no need on chromatographic column separation as well as a preparation method and application of the algal polysaccharide extract. The algal polysaccharide extract is prepared by the following steps: (1) crushing seaweed, performing ultrasonic treatment, filtering out a soak solution, and drying by distillation under reduced pressure; (2) adding an extracting agent into the pretreated seaweed raw material according to a solid-to-liquid ratio, leaching, filtering, concentrating filtrate under reduced pressure, and desalting by using an ion dialysis method to obtain an algal polysaccharide crude product; (3) carrying out fermentation culture; and (4) carrying out hydrolysis reaction, vacuum concentration, ultrafiltration, percolation and permeate recovery, nano-filtration and retentate recovery, drying and crushing to obtain the algal polysaccharides. The obtained algal polysaccharide does not need to be separated through a chromatographic column, the method is low in cost and easy and convenient to operate, and the production efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a seaweed polysaccharide extract and a preparation method and application thereof. Background technique [0002] Seaweed is rich in reserves and contains a variety of active ingredients, which have important applications in food, medicine, daily chemicals, fertilizers, feed and other fields. Therefore, the comprehensive utilization of seaweed has attracted more and more attention. Seaweed mainly contains lipids, proteins, polysaccharides and other active ingredients. If the active ingredients in seaweed can be separated and purified, the utilization rate of seaweed can be greatly improved. Among the ingredients contained in seaweed, seaweed polysaccharide and seaweed protein have the highest content, and their application is also the most extensive. [0003] Seaweed biologically active substances can be roughly divided into two types: one is a substan...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K8/73A61P17/00A61P31/02A61P31/04A61P31/10A61Q17/00A61Q19/00A61Q19/08A61Q19/10
Inventor 由永骅
Owner 山东洁壹选生物科技有限公司