A kind of cell-like multiphase emulsion photoenzyme system catalyzes the method of oil hydrolysis and decarboxylation

Abstract

The invention belongs to the field of chemical production and chemical synthesis, and discloses a method for catalyzing oil hydrolysis and decarboxylation by a cell-like multiphase emulsion photoenzyme system. Add soluble salt, polymer and / or hydrophilic alcohol, fatty acid photodecarboxylase and oil to the lipase solution to form a cell-like multiphase emulsion system; or add soluble salt, polymer and / or hydrophilic alcohol, fatty acid photodecarboxylase Decarboxylase and fatty acid are evenly mixed to form a cell-like multiphase emulsion system; the cell-like multiphase emulsion system is under stirring conditions, and the enzyme-catalyzed reaction is carried out under blue light irradiation. There are three layers, and the supernatant product is collected as the product after hydrolysis and decarboxylation. Lipase can catalyze the deep hydrolysis of glycerides, and fatty acid photodecarboxylase catalyzes the decarboxylation of free fatty acids, which can realize rapid reaction and intelligent control while releasing substrate inhibition, and establish an efficient, controllable, easy-to-recover enzyme and synchronous product Isolated novel multi-enzyme catalytic system.

Description

technical field [0001] The invention belongs to the field of chemical production and chemical synthesis, relates to enzyme separation and application technology, in particular to a method for catalyzing oil hydrolysis and decarboxylation by a cell-like multiphase emulsion photoenzyme system. Background technique [0002] Alkanes (alkenes) are compounds that play an important role in chemical production and chemical synthesis. They are non-renewable resources and can only be obtained by exploiting oil and natural gas in the short term. As a non-renewable resource, oil reserves are very limited. The traditional method for preparing alkanes (alkenes) generally uses metal ion catalysts, under high pressure, H 2 generated under the conditions. Compared with traditional chemical catalysis, enzymatic catalysis has the advantages of low energy consumption, low pollution, mild reaction conditions, and high selectivity, but it needs to be powered by expensive NADPH. In recent years...

AI Technical Summary

Problems solved by technology

[0003] Fatty acid photodecarboxylase is a kind of biological enzyme that only depends on photocatalysis, and the reaction process must have the presence of light, so the light transmission of the system limits its application The key factor, while the traditional immobilized carrier catalytic system has poor light transmittance, which limits the immobilization of fatty acid photodecarboxylase, so no effective immobilization method for reusing the enzyme has been reported.

The lipase that is matched with the cascade reaction is an interfacial enzyme, which needs a large interface area to exert its effective catalytic efficiency. At present, most of the catalytic systems are difficult to meet the catalytic requirements of two special enzymes (high permeability and high permeability). Photonic and catalytic interfaces), it is urgent to jump out of traditional thinking and build a new catalytic system to match it

Images