A kind of cell-like multiphase emulsion photoenzyme system catalyzes the method of oil hydrolysis and decarboxylation

A technology of multiphase emulsion and enzyme system, applied in the direction of fermentation and so on

A technology of multiphase emulsion and enzyme system, applied in the direction of fermentation and so on

CN113621657BActive Publication Date: 2022-06-14SOUTH CHINA UNIV OF TECH
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  • A kind of cell-like multiphase emulsion photoenzyme system catalyzes the method of oil hydrolysis and decarboxylation
  • A kind of cell-like multiphase emulsion photoenzyme system catalyzes the method of oil hydrolysis and decarboxylation
  • A kind of cell-like multiphase emulsion photoenzyme system catalyzes the method of oil hydrolysis and decarboxylation

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Experimental program
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Effect test

Embodiment 1

[0050] Take 0.05g lipase AYS and place in a transparent glass bottle, 0.24g dipotassium hydrogen phosphate, 1mL (0.975g) Tris-HCl (100mM, pH=8.5) solution, mix well, add 1.5mL (about 0.6g) large intestine Bacteria whole cells (containing CvFAP) were mixed evenly, and 0.233g of polyethylene glycol 400 and 0.05g of waste cooking oil were added, put into a interlayer beaker wrapped with an LED light device capable of producing blue light, and placed at a constant temperature of 1000rpm On the stirrer, the reaction was controlled at 30°C for 12h. After the reaction, add ethyl acetate twice the volume of the system for extraction, set 12000rpm to centrifuge for 3 minutes, and divide into three layers, which are the upper liquid layer, middle liquid layer and lower liquid layer in turn. After hydrolysis, free fatty acids and long Paraffins and unreacted esters are mainly enriched in the upper liquid layer, and the upper liquid layer product is collected as the product, and the resul...

Embodiment 2

[0053] Take 0.05g lipase AYS and place in a transparent glass bottle, 0.30g sodium sulfate, 1mL (0.975g) Tris-HCl (100mM, pH=8.5) solution, mix well, add 1.5mL (about 0.6g) whole Escherichia coli Cells (containing CvFAP) were mixed evenly, added 0.279g polyethylene glycol 600 and 0.05g camellia oil, put into a sandwich beaker wrapped with an LED light device capable of producing blue light, and placed on a constant temperature stirrer with a rotation speed of 1000rpm , Control the reaction at 30°C for 12h. After the reaction, add ethyl acetate twice the volume of the system for extraction, set 12000rpm to centrifuge for 3 minutes, and divide into three layers, which are the upper liquid layer, middle liquid layer and lower liquid layer in turn. After hydrolysis, free fatty acids and long Paraffins and unreacted esters are mainly enriched in the upper liquid layer, and the upper liquid layer product is collected as the product, and the resulting product is determined by GC ( ...

Embodiment 3

[0056] Take 0.05g lipase AYS and place in a transparent glass bottle, 0.24g dipotassium hydrogen phosphate, 1mL (0.975g) Tris-HCl (100mM, pH=8.5) solution, mix well, add 1.5mL (about 0.6g) large intestine Bacillus whole cells (containing CvFAP) were mixed evenly, added 0.285g polyethylene glycol 400, 0.05g soybean oil, put into a sandwich beaker wrapped with an LED light device capable of producing blue light, and placed in a constant temperature stirring at a speed of 1000rpm On the device, the reaction was controlled at 30°C for 12h. After the reaction, add ethyl acetate twice the volume of the system for extraction, set 12000rpm to centrifuge for 3 minutes, and divide into three layers, which are the upper liquid layer, the middle liquid layer and the lower liquid layer in turn. After hydrolysis, free fatty acids and long Paraffins and esters that have not participated in the reaction are mainly enriched in the upper liquid layer, and the product of the upper liquid layer i...

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Abstract

The invention belongs to the field of chemical production and chemical synthesis, and discloses a method for catalyzing oil hydrolysis and decarboxylation by a cell-like multiphase emulsion photoenzyme system. Add soluble salt, polymer and / or hydrophilic alcohol, fatty acid photodecarboxylase and oil to the lipase solution to form a cell-like multiphase emulsion system; or add soluble salt, polymer and / or hydrophilic alcohol, fatty acid photodecarboxylase Decarboxylase and fatty acid are evenly mixed to form a cell-like multiphase emulsion system; the cell-like multiphase emulsion system is under stirring conditions, and the enzyme-catalyzed reaction is carried out under blue light irradiation. There are three layers, and the supernatant product is collected as the product after hydrolysis and decarboxylation. Lipase can catalyze the deep hydrolysis of glycerides, and fatty acid photodecarboxylase catalyzes the decarboxylation of free fatty acids, which can realize rapid reaction and intelligent control while releasing substrate inhibition, and establish an efficient, controllable, easy-to-recover enzyme and synchronous product Isolated novel multi-enzyme catalytic system.

Description

technical field [0001] The invention belongs to the field of chemical production and chemical synthesis, relates to enzyme separation and application technology, in particular to a method for catalyzing oil hydrolysis and decarboxylation by a cell-like multiphase emulsion photoenzyme system. Background technique [0002] Alkanes (alkenes) are compounds that play an important role in chemical production and chemical synthesis. They are non-renewable resources and can only be obtained by exploiting oil and natural gas in the short term. As a non-renewable resource, oil reserves are very limited. The traditional method for preparing alkanes (alkenes) generally uses metal ion catalysts, under high pressure, H 2 generated under the conditions. Compared with traditional chemical catalysis, enzymatic catalysis has the advantages of low energy consumption, low pollution, mild reaction conditions, and high selectivity, but it needs to be powered by expensive NADPH. In recent years...

Claims

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Application Information

Patent Timeline
14 Jun 2022
Publication
CN113621657B
IPC
C12P5/02
CPC
C12P5/02
Inventors
李志刚; 梁倩