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Application of composition and kit thereof in detection of glycocholic acid

A composition, the technology of glycocholic acid, which is applied in the field of medical testing, can solve cumbersome problems, and achieve the effects of avoiding cumbersome processes, simple preparation procedures, and high sensitivity

Active Publication Date: 2021-11-30
SHENZHEN AMTECH BIOENGINEERING LTD INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Publication numbers are CN105301255B, CN105988000A, CN106841596A, CN108982860A, CN109444399A, CN109557301A, CN112285345A Chinese patents disclosed in the glycocholic acid detection reagent (latex immunoturbidimetric method), the competitor is glycocholic acid-protein or glycocholic acid-protein- Microspheres require activation, protein coupling, desalting and purification in the process of preparing the competitor; or activation, microsphere coupling, sealing, washing and other processes are very cumbersome

Method used

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  • Application of composition and kit thereof in detection of glycocholic acid
  • Application of composition and kit thereof in detection of glycocholic acid
  • Application of composition and kit thereof in detection of glycocholic acid

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preparation example Construction

[0096] In some embodiments, a preparation method of a glycocholic acid detection kit is provided, comprising:

[0097] 1) Preparation of Glycocholic Acid-Biotin

[0098] The preparation principle of glycochlic acid-biotin is as follows:

[0099]

[0100] Raw materials include glycocholic acid; NH 2 -PEG-Biotin is an amino PEG biotin derivative, and the molecular weight of PEG can be 300~3000; the condensing agent includes but not limited to N,N'-dicyclohexylcarbodiimide, 1-ethyl-(3-dimethyl Aminopropyl)carbodiimide hydrochloride, N,N'-diisopropylcarbodiimide, O-(7-azabenzotriazol-1-yl)-N,N,N', At least one of N'-tetramethyluronium hexafluorophosphate, benzotriazole-N,N,N',N'-tetramethyluronium hexafluorophosphate; catalysts include but not limited to 4-di At least one of methylaminopyridine, 1-hydroxybenzotriazole, 4-pyrrolidinylpyridine; solvents include but not limited to dichloromethane, tetrahydrofuran, diethyl ether, ethyl acetate, dimethyl sulfoxide, N,N -At least...

Embodiment 1

[0113] In this embodiment, the reagents used to adjust the pH are 2.0M hydrochloric acid and 2.0M sodium hydroxide aqueous solution.

[0114] In the present embodiment, the source and product information of each raw material are as follows:

[0115] Manufacturer of polystyrene carboxyl microspheres: JSR (Beijing Boermai agent); product name: latex microspheres; product number: P0113.

[0116] Glycocholic Acid Manufacturer: Aladdin; Product Name: Glycocholic Acid; Product Number: G131002.

[0117] Antibody: manufacturer: Tansen Technology; product name: CG rabbit polyclonal antibody; product number: FAB-F005.

[0118] NH 2 -PEG-Biotin: manufacturer: Shanghai Tuoyang; product name: biotin polyethylene glycol amino; product number: P002009.

[0119] 1. Preparation of Glycocholic Acid Reagent

[0120] 1.1 Preparation of Glycocholic Acid-Biotin Derivatives

[0121] Glycocholic acid (46.5 mg, 0.10 mmol), NH 2-PEG-Biotin (PEG molecular weight is about 2000, 240.0mg, about 0.12m...

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Abstract

The invention relates to application of a composition and a kit thereof in detecting glycocholic acid. The composition comprises a glycocholic acid-biotin derivative formed by combining glycocholic acid and biotin. The glycocholic acid detection kit is designed on the basis of an immunoturbidimetry platform, a synthesis method of glycocholic acid-biotin micromolecules is rapid, simple and convenient, a brand new reaction principle is designed by utilizing the combination characteristic of biotin-avidin combination, the defects in the prior art are overcome, the glycocholic acid-biotin micromolecule does not need to be combined with macromolecular protein or microspheres, and is directly applied to a latex immunoturbidimetric platform as a competitor, so that the tedious process of preparing the macromolecular competitor is avoided.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to the application of a composition and a kit thereof in detecting glycocholic acid. Background technique [0002] Cholylic acid (Cholyglycine, CG) is a conjugated cholic acid formed by combining cholic acid and glycine. Glycocholic acid is mainly synthesized in the liver and will be excreted into the intestinal tract with bile. Under normal circumstances, the concentration of Glycocholic acid in the blood is extremely low. [0003] The structural formula of glycocholic acid is as follows: [0004] [0005] When the liver cells are damaged, the ability of the liver cells to absorb glycocholic acid decreases, resulting in an increase in the content of glycocholic acid in the blood; when cholestasis occurs, the excretion of bile acid in the liver is hindered, and the content of glycocholic acid in the reflux blood circulation increases. It also increases the content of ...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/53G01N21/82
CPCG01N33/54346G01N33/5308G01N21/82G01N2800/085
Inventor 陈小茹周博王甜吴向东
Owner SHENZHEN AMTECH BIOENGINEERING LTD INC
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