Method for rapid fluorescence detection of chromium content

A technology for fluorescence detection and chromium content, which is applied in the field of chemical analysis and detection, can solve problems such as chromium pollution reduction or degradation, human health damage, and restrictions on the development of aquaculture industry, and achieve increased specificity, strong selectivity, and improved stability. Effect

Pending Publication Date: 2021-12-07
云南省生态环境厅驻昆明市生态环境监测站
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Problems solved by technology

However, it should be noted that the pollution caused by chromium is difficult to be reduced or degraded. Generally, it can only migrate between different areas or in different environments. During this migration process, due to changes in environmental conditions, chromium will It is inevitable that some changes in form or valence will occur, and the low concentration of chromium will be gradually enriched into high concentration of chromium, which will be absorbed by different levels of organisms in the food chain, resulting in different levels of accumulation, thereby restricting the development of aquaculture industry , causing unpredictable damage to human health

Method used

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  • Method for rapid fluorescence detection of chromium content
  • Method for rapid fluorescence detection of chromium content
  • Method for rapid fluorescence detection of chromium content

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Determination of Cr(Ⅵ) and Cr(Ⅲ) in environmental water samples

[0025] 1. Preparation of fluorescent probe: Mix 2 mL of 5 mmol / L histamine solution with 1 mL of 5 mmol / L tyramine solution, then add 5 mL of fluorescamine with a mass volume concentration of 0.02%, 3 mL of pH=8 phosphate buffer solution, and 1 mL of deionized water , vortexed for 1 min, and allowed to stand for 5 min to obtain a fluorescent probe solution;

[0026] 2. Creation of Cr(Ⅵ) working curve: add 100 µL of fluorescent probe and Cr(Ⅵ) standard solution into a 5 mL stoppered colorimetric tube, mix well and let stand for 3 minutes, dilute to 4 mL with deionized water, and mix well, in which Cr(Ⅵ) (Ⅵ) The concentration range in the stoppered colorimetric tube is 0.1-25mg / kg. Under the excitation wavelength of 390nm, the fluorescence intensity is measured at the wavelength of 488nm. For the fluorescence spectrum of Cr(Ⅵ) standard solution, see figure 1 , with the concentration of Cr(Ⅵ) as ...

Embodiment 2

[0039] Embodiment 2: Determination of Cr (Ⅵ) and Cr (Ⅲ) in soil

[0040] 1. Preparation of fluorescent probes

[0041] Mix 3mL of 5mmol / L histamine solution with 1mL of 5mmol / L tyramine solution, then add 6mL of fluorescamine with a mass volume concentration of 0.02%, 4mL of pH=8 phosphate buffer solution, 2mL of deionized water, vortex for 2min, and let stand 8min, obtain fluorescent probe solution;

[0042] 2. The production of Cr(Ⅵ) working curve is the same as in Example 1;

[0043] 3. Determination of Cr(Ⅵ) and Cr(Ⅲ) content in soil samples

[0044] (1) Sample treatment: Weigh 5g of soil, wash twice with 50mL acetone, then rinse with water, and dry at 30°C, then weigh 1.0g of dry soil, wash with 100mL HNO 3 ( 63%, m / v) and 50mL H 2 o 2 (30%, m / v) was dissolved, and reacted at 150±5°C for 2h, then washed with acid until nearly dry, and removed insoluble matter with a 0.22μm polytetrafluoroethylene filter membrane, took 5mL of the filtrate, and dilute it to 50mL with ...

Embodiment 3

[0047] Embodiment 3: the mensuration of chromium content in tap water sample

[0048] 1. Preparation of fluorescent probes

[0049] Mix 4 mL of 5 mmol / L histamine solution with 1 mL of 5 mmol / L tyramine solution, then add 7 mL of fluorescamine with a mass volume concentration of 0.02%, 5 mL of pH=8 phosphate buffer solution, 1.5 mL of deionized water, vortex for 1 min, and let Place for 10min to obtain the fluorescent probe solution;

[0050] 2. Cr ( ) Working curve making: with embodiment 1;

[0051] 3. Determination of Cr(Ⅵ) and Cr(Ⅲ) content in tap water: the operation is the same as in Example 1, and Cr(Ⅵ) and Cr(Ⅲ) were not detected;

[0052] Cr ( ) and Cr ( ) content determination method has the characteristics of simple operation, high sensitivity and rapidity, does not require large-scale equipment, and has strong advantages in actual detection.

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Abstract

The invention discloses a method for rapid fluorescence detection of chromium content. The method comprises the following steps: histamine, tyramine and fluorescamine are adopted to form a fluorescent derivative in a phosphoric acid buffer solution with pH of 8, the derivative generates strong fluorescence emission at 488nm under the excitation wavelength of 390nm, the characteristic that nitrogen and hydroxyl of the derivative have excellent affinity to Cr (VI) is utilized to generate specific complexation, Cr (VI) induces quenching of a fluorescence signal, and a chromium fluorescence probe is constructed and can be used for fluorescence detection of Cr (VI) in a sample; under the alkaline condition, Cr (III) is oxidized into Cr (VI) by hydrogen peroxide, the total chromium in the sample can be measured. The method can be used for morphological analysis of chromium, the detection limit of the method is 0.05 mg/kg, the method is high in specificity, and other metal ions do not have the phenomenon; The method is applied to detection of chromium in environmental food, a result accords with a related national standard determination method; the method has the characteristics of simple operation, high sensitivity, rapidness and the like.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a method for rapid fluorescence detection of chromium content. Background technique [0002] Chromium widely exists in the atmosphere, water, sediment and organisms, and chromium (Cr) mainly exists as Cr(Ⅲ) and Cr(Ⅵ). The positive significance and toxicity of chromium in the living body are related to its valence state. Cr (Ⅲ) plays a great role in maintaining the metabolism of glucose, lipid and protein, and is one of the essential trace elements for living organisms; while Cr ( Ⅵ) is an element that has a toxic effect on biological systems, and its toxicity is 100-1000 times greater than that of Cr (Ⅲ). In recent years, with the rapid development of industry and the continuous increase of human activities, most of the chromium pollution in the water environment is caused by the random discharge of a large amount of wastewater and solid resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/643G01N21/01
Inventor 杨健黄丽娟房晟忠夏万永李琳
Owner 云南省生态环境厅驻昆明市生态环境监测站
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