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Paenibacillus and application thereof in aspect of aerobic composting of chicken manure

A technology of Paenibacillus and Bacillus, which is applied to Paenibacillus and its application in chicken manure aerobic composting, can solve the problems of no application of chicken manure composting and fermentation, lack of products, etc., so as to reduce the loss of nitrogen in the heap and the total Nutrient loss, accelerated compost decomposing, and strong NH3 removal ability

Pending Publication Date: 2022-01-07
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the application of microbial agents in composting is still in its infancy, and there is a lack of effective products
[0003] However, the research on Paenibacillus is mainly for plant nitrogen fixation and biocontrol, and there is no report on its application in chicken manure composting fermentation

Method used

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  • Paenibacillus and application thereof in aspect of aerobic composting of chicken manure
  • Paenibacillus and application thereof in aspect of aerobic composting of chicken manure
  • Paenibacillus and application thereof in aspect of aerobic composting of chicken manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and Identification of Bacillus embodiment Class

[0037] 1. The isolated strain

[0038] A use of the medium to be separated from the Belleville natural compost sample, screening, identification give two Paenibacillus.

[0039] 2. The cell morphology and physiological and biochemical characteristics of

[0040] A single colony of strain 5-1 above characteristics (see figure 1 A): a diameter of 2.2mm, round colony morphology, colony lenticular convex surface, moist texture, smooth and rounded edges, colony color white, opaque, Gram staining.

[0041] Physiological strain 5-1 above physicochemical properties: optimum growth temperature 25 ℃ ~ 55 ℃, optimum growth temperature 35 ℃, can produce cellulases and amylases, may be utilized glucose, mannitol as a carbon source.

[0042] The main features of the above-described strain 6-6 as follows (see figure 1 B): colony diameter 1.9mm, dot colony morphology, colony lenticular convex surface, moist texture, smoo...

Embodiment 2

[0051] Example 2 Feed Paenibacillus (Paenibacillus pabuli) 5-1 and embodiment Paenibacillus station (Paenibacillus taichungensis) 6-6 degradation analysis

[0052] The feed Paenibacillus (Paenibacillus pabuli) 5-1 (abbreviated as strain 5-1) and Paenibacillus station (Paenibacillus taichungensis) 6-6 (abbreviated as strain 6-6) were seeded into point amylase medium and carboxymethylcellulose sodium methyl cellulose medium for each strain was repeated three cultured 24 ~ 48h 37 ℃.

[0053] And measuring the colony diameter clear zone, the mean value, the ratio of the size of the enzyme was a certain proportional relationship, the ability to compare enzyme producing strain is calculated transparent circle diameter than the diameter of the colony,

[0054] As shown in Table 2, Figure 4 , Figure 5 FIG: Strains Strain 5-1 and 6-6 were produced in the hydrolysis ring amylase and cellulase flat plates, strain 6-6 and 5-1 show that the strain can produce an amylase and a cellulase. These ...

Embodiment 3

[0057] Example 3 Example optimum growth temperature strain Research

[0058] In the composting process will undergo warming period, a high temperature stage, and cooling stage different temperatures, so that the strains are at 25 ℃, cultured at 35 ℃, 45 ℃, 55 ℃, 65 ℃, 70 ℃ environment, explore the strains may be grown temperature and the optimum growth temperature.

[0059] The results showed: 5-1 strain optimum growth temperature 25 ℃ ~ 55 ℃, optimum growth temperature 35 ℃. 6-6 strain optimum growth temperature 25 ℃ -55 ℃, optimum growth temperature 45 ℃.

[0060] Table 3 strains and the growth temperature optimum growth temperature range

[0061]

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Abstract

The invention discloses a paenibacillus and an application thereof in the aspect of aerobic composting of chicken manure, the paenibacillus is paenibacillus pabuli 5-1 or paenibacillus taichungensis 6-6, and the preservation number of the paenibacillus pabuli is CCTCC NO: M 2021574, and the preservation number of the paenibacillus taichungensis 6-6 is CCTCC NO: M2021575. The two paenibacillus strains are high in cellulase and amylase production capacity and strong in NH3 removal capacity, and the complex microbial inoculant prepared from the two paenibacillus strains can be used for aerobic composting of livestock and poultry manure, the compost temperature and the seed germination index can be increased, compost maturity is accelerated, compost ammonium nitrogen is promoted to be converted into nitrate nitrogen, compost nitrogen loss and total nutrient loss are remarkably reduced, and the compost organic matter content and the product quality are improved.

Description

Technical field [0001] The present invention relates to the field of microbiology applications, in particular to a Paenibacillus its application in the chicken manure composting. Background technique [0002] Composting is one of the major methods used manure harmless treatment, under aerobic conditions in livestock manure maturity faster than anaerobic conditions. Composting aerobic decomposition of organic substances in feces by microbial metabolism, inactivate fecal pathogens and parasites harmful substances harmless treatment purposes, having more thorough Slurry harmless treatment, fecal nutrient utilization, economic and good, have become the mainstream technology of animal manure resource utilization. But still warm Aerobic composting slow, long cycle, nutrient loss and severe odor volatile and serious problems, which lead to volatilization of ammonia nitrogen in significant loss of body pile, compost quality and efficiency to be further improved. Decomposition of organic ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C05F17/20C05F3/00C12R1/01C12R1/865
CPCC12N1/20C12N1/18C05F17/20C05F3/00Y02A40/20Y02W30/40
Inventor 吴健彭彦李展鑫任竹青黄逸豪张晟国王国磊张雨薇
Owner HUAZHONG AGRI UNIV