Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof
A technology for inhibiting peptides and low bitterness, applied in the field of deep processing of agricultural and sideline products, can solve problems such as severe bitterness, and achieve the effects of easy digestion and absorption, promoting sustainable development, and small molecular weight
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[0033] Example 1
[0034] A method of preparing a low bitter ACE inhibiting peptide from skim sesame powder, which includes the following steps:
[0035] 1) Exase fat sesame powder preparation:
[0036] The sesatist cake was pulverized, and degreasing was carried out by a rope extract, and the solvent was selected as petroleum ether (boiling point 40-60 ° C), and the extraction temperature was 60 ° C, and the degreasing 7 h was separated. The protoxed sesame melamine raw material was pulverized, and the mesh is collected, which is the degreasing sesame powder, and the protein content is 51.23% by the detection of sesame nitrogen (GB5009.5-2003);
[0037] 2) Protein enzymatic solution:
[0038] Step 1) The resulting degreasing sesame powder is mixed with 10 g: 100 mL of the distilled water. The pH was adjusted with 6 mol / lnaOh, then 9000 U / g was added under 50-55 ° C. 原料蛋白 Alkaline protease Alcalase, after 50-55 ° C, add 4000 U / g 原料蛋白 Flavor protease, continuing the enzymatic...
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[0044] Example 2
[0045] A method of artificial synthesis of low bitter sesame ACE inhibits peptide, which is composed of solid phase synthesis. The basic process is as follows: First, an amino acid is attached to the immunogenic acid from the FMoc group in the insoluble solid support WANG resin, then remove the protective group of the amino group, the first amino acid is attached to the solid support; secondly, the amino group is fmoc The second amino acid carboxyl group protected by the group is activated, and the activated amino acid is formed to form a peptide bond at an amino acid having a first amino acid that has been connected to the solid phase. Toppeptide with protective group. The above peptide bonds were repeated, allowing the peptide chain from the C-terminal n-end until the desired peptide chain length was reached, and finally the peptide was obtained. The method synthesized by the low bitter sesame ACE inhibits peptide Trp-Leu-Tyr-Arg (WLYR), which is shown in liqu...
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