Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof

A technology for inhibiting peptides and low bitterness, applied in the field of deep processing of agricultural and sideline products, can solve problems such as severe bitterness, and achieve the effects of easy digestion and absorption, promoting sustainable development, and small molecular weight

Pending Publication Date: 2022-01-11
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ACE inhibitory peptides often produce severe bitter taste due to the exposure of hydrophobic groups durin

Method used

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  • Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof
  • Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof
  • Sesame-sourced low-bitterness ACE inhibitory peptide as well as preparation method and application thereof

Examples

Experimental program
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Example Embodiment

[0033] Example 1

[0034] A method of preparing a low bitter ACE inhibiting peptide from skim sesame powder, which includes the following steps:

[0035] 1) Exase fat sesame powder preparation:

[0036] The sesatist cake was pulverized, and degreasing was carried out by a rope extract, and the solvent was selected as petroleum ether (boiling point 40-60 ° C), and the extraction temperature was 60 ° C, and the degreasing 7 h was separated. The protoxed sesame melamine raw material was pulverized, and the mesh is collected, which is the degreasing sesame powder, and the protein content is 51.23% by the detection of sesame nitrogen (GB5009.5-2003);

[0037] 2) Protein enzymatic solution:

[0038] Step 1) The resulting degreasing sesame powder is mixed with 10 g: 100 mL of the distilled water. The pH was adjusted with 6 mol / lnaOh, then 9000 U / g was added under 50-55 ° C. 原料蛋白 Alkaline protease Alcalase, after 50-55 ° C, add 4000 U / g 原料蛋白 Flavor protease, continuing the enzymatic...

Example Embodiment

[0044] Example 2

[0045] A method of artificial synthesis of low bitter sesame ACE inhibits peptide, which is composed of solid phase synthesis. The basic process is as follows: First, an amino acid is attached to the immunogenic acid from the FMoc group in the insoluble solid support WANG resin, then remove the protective group of the amino group, the first amino acid is attached to the solid support; secondly, the amino group is fmoc The second amino acid carboxyl group protected by the group is activated, and the activated amino acid is formed to form a peptide bond at an amino acid having a first amino acid that has been connected to the solid phase. Toppeptide with protective group. The above peptide bonds were repeated, allowing the peptide chain from the C-terminal n-end until the desired peptide chain length was reached, and finally the peptide was obtained. The method synthesized by the low bitter sesame ACE inhibits peptide Trp-Leu-Tyr-Arg (WLYR), which is shown in liqu...

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Abstract

The invention belongs to the technical field of deep processing of agricultural and sideline products, and particularly relates to a low-bitterness sesame ACE (angiotensin converting enzyme) inhibitory peptide which consists of four amino acids and has the sequence of Trp-Leu-Tyr-Arg (WLYR). The low-bitterness sesame polypeptide can be obtained through proteolysis of degreased sesame powder, debitterizing, separation and purification, and can also be artificially synthesized through adoption of a chemical solid-phase synthesis method. Sensory evaluation, electronic tongue analysis and ACE inhibition capability determination show that the polypeptide basically has no bitter taste, has strong antihypertensive activity, belongs to food-borne antihypertensive peptides, has small molecular weight, is easy to absorb, can be used as a functional active component for preparing antihypertensive drugs, and can be used as active drugs in the fields of health care products, food, medicines and the like, snd the market prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural and sideline products, and specifically relates to a low-bitter ACE inhibitory peptide WLYR derived from defatted sesame powder, a preparation method and its application in blood pressure-lowering drugs, health products, food, etc. Background technique [0002] Hypertension is the main cause of coronary heart disease, arteriosclerosis, stroke, and heart failure. According to epidemiological studies, more than 1 billion people now suffer from high blood pressure. Therefore, the prevention and treatment of hypertension has become a global public problem to be solved urgently. Angiotensin I transferase (ACE) is a dipeptide carboxypeptidase that plays an important role in blood pressure regulation. At present, the drugs for the treatment of hypertension mainly include sartan and dipine, but these chemically synthesized drugs often cause side effects such as cough, rash, and h...

Claims

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Application Information

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IPC IPC(8): C07K5/117C12P21/06C07K1/34C07K1/16C07K1/14
CPCC07K5/1024C12P21/06
Inventor 贾聪芦鑫孙强黄纪念
Owner HENAN ACAD OF AGRI SCI
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