Unlock instant, AI-driven research and patent intelligence for your innovation.

Psychrobacter cryohalolentis, and application thereof in cold storage and preservation of vegetables

A psychrophilic bacillus and salt crystal technology, applied in the field of food preservatives, feed biological additives, and microorganisms, can solve the problem of hindering the mining and understanding of bacteriocin-producing bacteria resources, and it is difficult to perform at temperatures below 10°C or even below 4°C. Antibacterial effect and other problems, to achieve the effect of increasing the scope of application, wide antibacterial spectrum, strong acid and alkali resistance

Inactive Publication Date: 2022-01-28
青岛新万福食品有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing bacteriocin-producing bacteria have problems such as limited strain resources, poor antibacterial properties, and single applicable conditions for antibacterial
Moreover, since most of the current researches only focus on the bacteriocin-producing bacteria of the genus Lactobacillus and Bacillus, less attention has been paid to the bacteriocin-producing bacteria of other groups, and most of the strains that can secrete bacteriocins have been screened. It is a strain that can efficiently ferment biotin to achieve antibacterial effect at room temperature or sub-high temperature (>40°C), and it is difficult to exert effective effect in the cold storage of fruits and vegetables (generally below 10°C, even below 4°C). antibacterial effect
Due to the above-mentioned technical limitations, people are hindered from excavating and understanding the resources of bacteriocin-producing bacteria.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Psychrobacter cryohalolentis, and application thereof in cold storage and preservation of vegetables
  • Psychrobacter cryohalolentis, and application thereof in cold storage and preservation of vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening of Psychrobacterium halocrystals SDWF2-4

[0025] Take the fruit and vegetable samples (temperature 4°C) that have been stored for a long time in the vegetable refrigerated and cold storage of our company without mildew or spoilage, and use sterile water to wash the surface on the ultra-clean workbench, at 10°C, 150rpm / After 20 days of enrichment at 1 min, the LB medium was continuously replenished. After the OD600 value of the bacterial solution reached above 0.5, it was serially diluted and inoculated on a solid medium, cultivated at 10°C for 6 days, and a typical single colony was selected for purification.

[0026] Among them, since the screening target bacteria are unknown, four common screening media in this field were used, namely: LB nutrient medium, lactic acid bacteria medium (Lactic Acid Bacteria Culture Medium, MRS), beef extract peptone medium (Beef Extract Peptone Medium, NA), Columbia Blood Agar Medium (Columbia BloodAgar Medium, CA)....

Embodiment 2

[0035] Example 2 Preparation of Bacteriocins by Fermentation of Psychrobacterium halocrystals SDWF2-4

[0036] Start from the single colony screened and preserved in Example 1, pick a single colony of the bacteria and inoculate it in LB medium, culture it with shaking at 5-25°C for 4-24 hours, centrifuge the culture solution, remove the bacteria, and keep the supernatant , use sodium hydroxide supernatant to adjust the pH value to be consistent with that of distilled water, and then filter through a 0.22 μm filter membrane for later use; put the filtered clear liquid in a beaker, add fully ground ammonium sulfate, and stand for dialysis after reaction , to obtain the crude extract of Psychrobacterium halocrystals SDWF2-4 bacteriocin, dry the crude extract and add different compounding agents to prepare antibacterial agents, which are subpackaged for use.

[0037] The Oxford cup double-layer plate method commonly used in this field was used for screening, and Staphylococcus aur...

Embodiment 3

[0041] The property detection of embodiment 3 bacteriocin

[0042] Carry out following experiment after the bacteriocin crude extract obtained in embodiment 2 is subpackaged:

[0043] (1) thermal stability test

[0044] Put the above-mentioned bulk liquid in water bath at 5°C, 10°C, 20°C, 30°C, 40°C, 50°C, 60°C, 70°C, and 80°C for 20min, 40min, and 60min respectively. a parallel group. The antibacterial activity of bacteriocin was determined by double-layer plate method with Staphylococcus aureus as indicator bacteria.

[0045] The results show that bacteriocins have certain activity in the range of 5-60°C, but the activity is high or low. Specifically, the activity is the strongest at 20-30°C. After 1 hour, the original activity is basically maintained, which can realize the stable maintenance of antibacterial activity; under the action of 60°C, the effect will be weakened, and it will begin to decline after 20 minutes of treatment, and the decline will be obvious after 1 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a Psychrobacter cryohalolentis, and application thereof in cold storage and preservation of vegetables. Bacteriocin produced by the Psychrobacter cryohalolentis SDWF2-4 has obvious antibacterial activity, wide antibacterial spectrum, good thermal stability, low temperature resistance, strong acid and alkali resistance and wide pH application range. The Psychrobacter cryohalolentis SDWF2-4 and the bacteriocin of the Psychrobacter cryohalolentis SDWF2-4 are obtained from the nature, industrial production is easy to carry out, and the cost of a medicine or a disinfectant prepared from the Psychrobacter cryohalolentis SDWF2-4 or the bacteriocin can be reduced; moreover, the problem of drug residue does not need to be considered, and the compound can be widely applied as a safe and efficient biological disinfectant and drug.

Description

technical field [0001] The present invention relates to the field of microbiology and medicine, more specifically, to a strain of bacteriocin-producing psychrophilic bacillus, the fermentation method of the bacteriocin, the method for extracting bacteriocin and its application, suitable for Food preservatives, feed biological additives. Background technique [0002] With the improvement of people's living standards and the continuous improvement of the demand for nutrition and health, the demand for fresh fruits and vegetables is becoming stronger and stronger. In order to meet the above actual needs, fresh fruits and vegetables become fresh-cut fruits and vegetables after screening, cleaning and slicing after picking. It is a lightly processed product that is more and more popular and loved by consumers. Although this kind of ready-to-eat fruit and vegetable caters to consumers' concept of healthy diet, the product is easily contaminated during processing, which provides g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N1/02C12P21/00A23B7/155A01N63/20A01P3/00A01P1/00C12R1/01
CPCC12N1/20C12N1/02C12P21/00C07K14/195A23B7/155A01N63/20A23V2002/00A23V2200/10
Inventor 史蕾刘成阳左常熙逄明祝郑大川李海松吴英旗左晟晨
Owner 青岛新万福食品有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More