Pediococcus acidilactici for improving production performance and immune level of broiler chickens and screening method and application thereof

A technology of Pediococcus lactis and performance, applied in the field of microorganisms, can solve the problems of reducing the average daily weight gain and feed conversion rate of broilers

Active Publication Date: 2022-02-01
TIANJIN BIOFEED TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wang Lihong (Isolation and Screening of Chicken-derived Lactic Acid Bacteria and Its Effects on Broiler Performance [D]. 2014) once isolated and screened a strain of Pediococcus lactis, and found that the strain significantly reduced the average daily gain and feed conversion rate of broilers

Method used

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  • Pediococcus acidilactici for improving production performance and immune level of broiler chickens and screening method and application thereof
  • Pediococcus acidilactici for improving production performance and immune level of broiler chickens and screening method and application thereof
  • Pediococcus acidilactici for improving production performance and immune level of broiler chickens and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1, have broad-spectrum bacteriostasis and stress-resistance characteristic Pediococcus lactis screening method

[0085] 1. Isolation and purification of bacterial strains

[0086] Thirty-five cases of digesta samples from the rumen of dairy cows, chicken cecum, pig ileum, and rabbit cecum that were healthy and in good growth condition were collected for screening the target strains. The specific method is: take 10 g of the above-mentioned samples, dissolve them in 90 mL of sterile PBS solution, shake at a constant temperature of 160 rpm for 20 minutes, absorb 500 μL and transfer them into 5.5 mL of MRS liquid medium, and enrich and culture them at 37 ° C and 160 rpm for 24 hours. Then draw 100 μL of the bacterial liquid, inoculate it on the MRS solid medium, culture it statically at 37°C for 24 hours, use the plate dilution method to separate and purify, select a single colony on the MRS solid medium for three generations of purification, and store it in 50% ...

Embodiment 2

[0102] Example 2. Safety evaluation and identification of LC-9-1 bacterial strain with broad-spectrum antibacterial and stress resistance properties

[0103] 1. Safety evaluation

[0104] 1. Antibiotic sensitivity test

[0105] The test adopts the drug-sensitive disc agar diffusion method. Take 100 μL of the activated LC-9-1 bacterial suspension and spread it on the MRS solid medium. After the bacterial solution is completely absorbed, put the drug-sensitive disc on the medium. Above, after stabilizing for 15 minutes, incubate upside down at 37°C for 20 hours, measure the diameter of the inhibition zone and calculate the area, and judge the results according to the "CLSI Antimicrobial Susceptibility Test Standard".

[0106] The preparation method of the above-mentioned LC-9-1 bacterial suspension is as follows: inoculate the isolated and purified strain LC-9-1 in MRS solid medium, and after solid culture for 24 hours, pick a single colony and inoculate it into 10 mL of MRS li...

Embodiment 3

[0127] Embodiment 3, the impact of Pediococcus lactis LC-9-1 on broiler growth performance and immune performance

[0128] 1. Feeding experiment of broiler chickens with different gradients of Pediococcus lactis LC-9-1

[0129] Select 300 1-day-old healthy Aiba Yijia broiler chickens (AA broilers), and randomly divide them into 5 treatment groups, each treatment group has 6 replicates, and each replicate has 10 chickens. Grouping and dietary composition: control group CON Feeding basal rations; Pediococcus lactis LC-9-1 groups PA1, PA2, and PA3 respectively added Pediococcus lactis LC-9-1 bacterial suspension to the basal rations, so that the bacterial concentrations in the basal rations were 1.0 ×10 7 CFU / kg, 1.0×10 8 CFU / kg, 1.0×10 9 CFU / kg; AGPs group added combination antibiotics (chlortetracycline 100 mg / kg, kitasarmycin 20 mg / kg) on ​​the basis of basal diet. The basal diet is a corn-soybean meal type diet, and the formula design refers to the "Chicken Feeding Standa...

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Abstract

The invention discloses pediococcus acidilactici for improving the production performance and immune level of broiler chickens and a screening method and application thereof. The invention provides a novel pediococcus acidilactici screening method and pediococcus acidilactici LC-9-1 screened by the method by improving a screening procedure and adopting an in-vitro and in-vivo combined strategy. In-vivo tests prove that the pediococcus acidilactici LC-9-1 has the following application effects: the safety is high, the stress resistance is strong, the pediococcus acidilactici LC-9-1 is easy to colonize in intestinal tracts, the intestinal flora structure can be improved, the development of the intestinal tracts can be promoted, the pathological injury of the liver and the spleen can be relieved, the growth performance and the immune performance can be improved, and the effect of replacing AGPs can be achieved to a certain extent.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Pediococcus lactis capable of improving the production performance and immune level of broiler chickens, a screening method and application thereof. Background technique [0002] In the process of poultry feeding, chicks with immature intestinal function have low immune function and are easily disturbed by external factors such as pathogenic bacteria, nutrition and environment, which is an important reason for poor growth performance and high death rate . Antibiotics (AGPs) have been widely used in animal feed as growth-promoting additives, and are used to treat and prevent pathogenic bacterial infections in poultry farming, effectively improving production efficiency. However, more and more studies have shown that the abuse of AGPs will lead to the aggravation of drug resistance and cause antibiotic residues in the environment, spread through the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75A61K35/744A61P31/04A61P3/00A61P37/04A61P1/00A61P1/16C12R1/01
CPCA23K10/18A23K50/75A61K35/744A61P31/04A61P3/00A61P37/04A61P1/00A61P1/16A23V2400/413Y02A50/30
Inventor 李冲邓雪娟蔡辉益李爽王少龙王腾飞
Owner TIANJIN BIOFEED TECH CO LTD
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