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Method for extracting high-purity honeysuckle chlorogenic acid by membrane separation method

A technology of honeysuckle chlorogenic acid and membrane separation method, which is applied in separation methods, semi-permeable membrane separation, microorganism-based methods, etc., can solve the problems of difficulty in practical promotion, poor separation and purification effect, complicated operation, etc., so as to reduce production. Risks and production costs, broad market application prospects, the effect of efficient extraction

Pending Publication Date: 2022-02-11
LINYI ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the current extraction and purification process of honeysuckle chlorogenic acid all has the disadvantages of low extraction rate of raw materials, cumbersome procedures, complicated operation, poor separation and purification effect, and the yield and purity of the obtained chlorogenic acid are not very ideal, resulting in chlorogenic acid extraction. The production cost is extremely high, and there are great difficulties in actual promotion

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  • Method for extracting high-purity honeysuckle chlorogenic acid by membrane separation method
  • Method for extracting high-purity honeysuckle chlorogenic acid by membrane separation method
  • Method for extracting high-purity honeysuckle chlorogenic acid by membrane separation method

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Embodiment 1

[0037] A method for extracting high-purity honeysuckle chlorogenic acid by membrane separation method. Chlorogenic acid is prepared by crushing enzymolysis, heating extraction, macroporous resin adsorption, membrane separation, and spray drying, which specifically includes the following steps:

[0038] (1) Crushing and enzymatic hydrolysis: crush the dried honeysuckle, add water, add compound enzyme, mix and soak for 12 hours at 28-30°C;

[0039] (2) Heat leaching: adjust the pH to 3-4 after immersion, soak again for 12 hours; then heat leaching, the leaching temperature is 70°C, and the leaching time is 1.0 hour, the material is separated from solid and liquid, and the liquid is centrifuged , centrifuge speed 5000r / min centrifugal treatment 20min, obtain extract;

[0040] (3) Macroporous resin adsorption: adjust the pH of the extract to 3-4, add a macroporous resin column for saturated adsorption, remove impurities with water and a low-concentration ethanol solution, and then...

Embodiment 2

[0053] A method for extracting high-purity honeysuckle chlorogenic acid by membrane separation method. Chlorogenic acid is prepared by crushing enzymolysis, heating extraction, macroporous resin adsorption, membrane separation, and spray drying, which specifically includes the following steps:

[0054] (1) Crushing and enzymatic hydrolysis: crush the dried honeysuckle, add water and compound enzyme, mix and soak for 24 hours at 28-30°C;

[0055] (2) Heating leaching: after soaking, adjust the pH to 3-4, soak again for 24 hours; then heat leaching, the leaching temperature is 90°C, and the leaching time is 2.0 hours, the material is separated from solid and liquid, and the liquid is centrifuged , centrifuge speed 5000r / min centrifugal treatment 20min, obtain extract;

[0056] (3) Macroporous resin adsorption: adjust the pH of the extract to 3-4, add a macroporous resin column for saturated adsorption, remove impurities with water and a low-concentration ethanol solution, and th...

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Abstract

The invention discloses a method for extracting high-purity honeysuckle chlorogenic acid through a membrane separation method, and belongs to the technical field of plant extraction. The high-purity chlorogenic acid is prepared through crushing and enzymolysis, heating and extraction, macroporous resin adsorption, membrane separation and spray drying. Firstly, honeysuckle is extracted in an enzymolysis and water extraction mode, used cellulase can decompose honeysuckle cell walls, aspergillus niger pectinase can keep high activity in a soft acid environment, dissolution of effective substances of the honeysuckle can be efficiently promoted in cooperation with use of the cellulase, and a substance premise is provided for a subsequent membrane separation and purification link; secondly, macroporous resin elution is conducted on the water extract, the purposes of concentration and purification are achieved in combination with membrane separation, the membrane separation technology is combined with the water extraction and enzymolysis technology, efficient extraction of honeysuckle chlorogenic acid is achieved, no organic solvent is used, the production risk and the production cost are greatly reduced, and the method is green, free of pollution and wide in market application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting high-purity honeysuckle chlorogenic acid by a membrane separation method. Background technique [0002] Honeysuckle (Lonicera japonica) is the early blooming flower or dried flower bud of honeysuckle. It has anti-pathogenic microorganisms, anti-inflammation, antipyretic, liver protection, blood lipid lowering, hemostasis, immune regulation, etc. It is mainly used clinically to treat various infections, Inflammation, hyperlipidemia, tumor radiotherapy, etc. [0003] Honeysuckle contains aromatic oil, chlorogenic acid, flavonoids, saponins and tannins and other biologically active components. It is an important medicinal and edible plant raw material and is widely used in the food industry and pharmaceutical industry. Chlorogenic acid is a typical antioxidant component in honeysuckle. It has strong pharmacological activity and has signi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/60B01D61/58C07C69/732B01D15/00C12N1/14C12N9/18C12N9/26C12N9/88C12R1/685
CPCC07C67/48C07C67/60B01D61/58B01D15/00C12N9/88C12N1/14C12N9/2408C12N9/18C12Y301/01011C12Y302/01015C12Y402/02002C07C2601/14C07B2200/07C07C69/732
Inventor 丁文静陈士国姚现琦郭绍芬李静杜元伟刘雪平穆清泉孙继芳郭艳萍杨宝山魏景亮邰宗军李西坤徐建军
Owner LINYI ACADEMY OF AGRI SCI