Composite microbial agent for sewage treatment and preparation method thereof

A microbial bacterial agent and sewage treatment technology, which is applied in the field of compound microbial bacterial agent and its preparation, can solve the problems of black and smelly sewage, poor treatment effect, denaturation, etc., and achieves the reduction of foul smell, large volume, and easy fishing Effect

Inactive Publication Date: 2022-02-11
GUANGDONG XINTAILONG ENVIRONMENTAL PROTECTION GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under the decomposition of microorganisms, organic matter will produce hydrogen sulfide and other substances, resulting in black and smelly sewage, and the sewage may also contain heavy metal ions such as mercury and chromium. If the sewage is treated with conventional microbial agents, these heavy metal ions will be combined in the The surface of the bacteria cells in the bacterial agent will cause protein denaturation and kill the bacteria, so the treatment effect is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Prepare the desulfurization bacterial strain, comprising the steps of:

[0025] Step 1: Mix distilled water, sodium dihydrogen phosphate, ammonium chloride, sodium carbonate, sodium sulfide and magnesium chloride hexahydrate according to the dosage ratio of 1L: 2g: 0.3g: 0.3g: 0.16g: 0.3g to prepare the initial medium ; According to the dosage ratio of 1L: 3g: 10g: 5g: 0.05g, mix distilled water, beef extract, peptone, sodium chloride and sodium sulfide to prepare an activation medium; prepare the activation medium, mercury nitrate and chromium nitrate according to 1L: Mix the dosage ratio of 0.05mmol:0.5mg to prepare the screening medium; mix the activation medium and agar powder according to the dosage ratio of 100g:2g to obtain a solid medium;

[0026] Step 2: Add air-dried pig manure to the initial medium, and culture it statically at 28°C for 24 hours, so that the desulfurization bacteria in the air-dried pig manure can consume the sulfur element in the initial med...

Embodiment 2

[0030] Prepare the desulfurization bacterial strain, comprising the steps of:

[0031] Step 1: Mix distilled water, sodium dihydrogen phosphate, ammonium chloride, sodium carbonate, sodium sulfide and magnesium chloride hexahydrate according to the dosage ratio of 1L: 2.5g: 0.4g: 0.4g: 0.16g: 0.35g to prepare the initial culture According to the dosage ratio of 1L: 3.5g: 10g: 5g: 0.05g, distilled water, beef extract, peptone, sodium chloride and sodium sulfide were mixed to prepare an activation medium; the activation medium, mercury nitrate and chromium nitrate were mixed according to 1L: 0.05mmol: 0.5mg was mixed to prepare a screening medium; the activation medium and agar powder were mixed according to the dosage ratio of 100g: 2.5g to obtain a solid medium;

[0032] Step 2: Add air-dried pig manure to the initial medium, and culture it statically at 30°C for 36 hours, so that the desulfurization bacteria in the air-dried pig manure can consume the sulfur element in the in...

Embodiment 3

[0036] Prepare the desulfurization bacterial strain, comprising the steps of:

[0037] Step 1: Mix distilled water, sodium dihydrogen phosphate, ammonium chloride, sodium carbonate, sodium sulfide and magnesium chloride hexahydrate according to the dosage ratio of 1L: 3g: 0.5g: 0.5g: 0.16g: 0.4g to prepare the initial medium ;According to the dosage ratio of 1L:4g:10g:5g:0.05g, mix distilled water, beef extract, peptone, sodium chloride and sodium sulfide to prepare an activation medium; prepare the activation medium, mercury nitrate and chromium nitrate according to 1L: Mix the dosage ratio of 0.05mmol:0.5mg to prepare the screening medium; mix the activation medium and agar powder according to the dosage ratio of 100g:3g to obtain a solid medium;

[0038] Step 2: Add air-dried pig manure to the initial medium, and culture it statically at 32°C for 48 hours, so that the desulfurization bacteria in the air-dried pig manure can consume the sulfur element in the initial medium; ...

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Abstract

The invention discloses a composite microbial agent for sewage treatment and a preparation method thereof and belongs to the technical field of environment-friendly agents.A plurality of obtained single colonies is further screened through an activation culture medium containing sulfur ions, and the obtained desulfurization bacterial strain has a good desulfurization function. The compound microbial agent effectively reduces the foul smell of treated sewage, can survive in sewage containing heavy metals, and improves the treatment effect of the prepared compound microbial agent on sewage containing complex components. The porous resin is used for absorbing and coating the composite bacterial liquid, nutrient substances which are not completely fermented and digested in the composite bacterial liquid can be absorbed together with the composite bacterial strains and are well retained in the dry porous resin, and when the composite microbial agent is used, the composite microbial agent firstly absorbs moisture in sewage and expands so that the nutrient substances are dissolved and the compound strains are activated.

Description

technical field [0001] The invention belongs to the technical field of environmental protection agents, and in particular relates to a composite microbial agent for sewage treatment and a preparation method thereof. Background technique [0002] Sewage includes domestic sewage, industrial wastewater and runoff sewage. Sewage contains a large amount of organic matter, including carbohydrates, proteins, amino acids, fatty acids, oils, esters and other substances. These organic matters will make the sewage water eutrophic and breed a large number of bacteria. Under the decomposition of microorganisms, organic matter will produce hydrogen sulfide and other substances, resulting in black and smelly sewage, and the sewage may also contain heavy metal ions such as mercury and chromium. If the sewage is treated with conventional microbial agents, these heavy metal ions will be combined in the The cell surface of the bacteria in the bacterial agent will cause protein denaturation and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C12N11/087C12N1/20C12N1/16C02F3/34C12R1/01C12R1/125C12R1/645C02F101/20C02F101/10
CPCC12N11/12C12N11/087C12N1/20C12N1/16C02F3/34C02F3/345C02F3/347C02F2101/20C02F2101/101
Inventor 辛永光欧宏森
Owner GUANGDONG XINTAILONG ENVIRONMENTAL PROTECTION GRP CO LTD
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