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Pediococcus pentosaceus He10-a-1 and application thereof

A technology of he10-a-1 and Pediococcus pentosaceae, applied in the field of Pediococcus pentosaceae He10-a-1, can solve the problems that have not been reported, and achieve a wide range of Application prospects, scavenging DPPH and OH · hydroxyl radicals, and the effect of inhibiting lipid peroxidation

Pending Publication Date: 2022-02-25
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Pediococcus pentosacea has not been reported in the prevention and treatment of heavy metals

Method used

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  • Pediococcus pentosaceus He10-a-1 and application thereof
  • Pediococcus pentosaceus He10-a-1 and application thereof
  • Pediococcus pentosaceus He10-a-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0144] Embodiment 1: Isolation and Identification of Pentosan Pediococcus Strains of the Present Invention

[0145] experiment method:

[0146] Purified Pediococcus pentosaceae He10-a-1 was preserved in glycerol and vacuum frozen. The activated 3rd generation Pediococcus pentosaceae He10-a-1 was tested for glucose gas production, catalase test, pH value growth test, temperature growth test, salt tolerance test, nitrate reduction test, gelatin liquefaction test, sugar fermentation test Physiological and biochemical tests, etc.

[0147] Each of these physiological and biochemical tests will be described below.

[0148] (i) Glucose gas production test

[0149] Take 1 drop of the third-generation Pediococcus pentosaceae He10-a-1 bacteria solution and inoculate it into a biochemical identification tube with a built-in Duchenne small catheter, and incubate at 37°C for 18-24 hours. Observation found that if there are air bubbles in Duchenne's ductus, it is positive, which means h...

Embodiment 2

[0164] Embodiment 2: the acid resistance test of bacterial strain of the present invention

[0165] The implementation mode of this embodiment is as follows:

[0166] Using sterile normal saline, the culture of the second-generation Pediococcus pentosaceae He10-a-1 was centrifuged and washed at 4000r / min for 10 min, washed 3 times, and its bacterial strain was made into 1 × 10 with 0.85% normal saline. 7 CFU / mL for the test bacteria solution, 2% of the volume of the MRS liquid medium is added to the MRS liquid medium with a pH of 2.5, 3.5, 4.5, 5.5, and 6.5, and cultivated in an incubator at a temperature of 37 ° C for 24 hours. Take a sample and measure its OD by UV-Vis spectrophotometry 595nm Value, according to two parallel triplicate experiments, by analyzing the effect of acidity on the growth of Pediococcus pentosaceae He10-a-1.

[0167] The implementation result of this embodiment is listed in table 4.

[0168] Table 4: OD of Pediococcus pentosaceae He10-a-1 strain a...

Embodiment 3

[0172] Embodiment 3: the artificial bile salt resistance test of bacterial strain of the present invention

[0173] The implementation mode of this embodiment is as follows:

[0174] Utilize aseptic normal saline to carry out centrifugation wash 10min under the condition of rotating speed 4000r / min of the second generation Pediococcus pentosaceae He10-a-1 culture, wash 3 times, make its bacterial strain 1 × 10 7 CFU / mL for the test bacteria solution, the MRS liquid medium containing 0.1%, 0.2%, and 0.3% bile salts in grams / liter was inserted into the MRS liquid medium containing 0.1%, 0.2%, and 0.3% bile salt in grams / liter at a rate of 2% based on the volume of the MRS liquid medium. 2% of the volume of the culture medium was inserted into the MRS liquid medium without bile salts as the control group, cultured in the incubator at a temperature of 37°C for 24 hours, and samples were taken, and the corresponding blank medium was used as the control using a microplate reader. T...

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Abstract

The invention relates to a Pediococcus pentosaceus He10-a-1 and application thereof. The Pediococcus pentosaceus He10-a-1 is used for preparing a lactic acid bacteria microbial preparation with high tolerance and high adsorption of heavy metal lead. A preparation method comprises the steps of strain activation, lactic acid bacteria amplification culture, centrifugal separation, protective agent preparation, protective agent addition, freeze drying and the like. The lactic acid bacteria microbial preparation with high tolerance and high adsorption of heavy metal lead has efficient removal capacity on heavy metal lead, and after mice are fed with a Pediococcus pentosaceus liquid, behavior indexes, serum biochemical indexes and intestinal indexes are normal, and the Pediococcus pentosaceus liquid is safe and reliable to eat.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology. More specifically, the present invention relates to a Pediococcus pentosaceus He10-a-1, and also relates to the use of the Pediococcus pentosaceus He10-a-1. 【Background technique】 [0002] The heavy metal pollution in some areas at home and abroad is quite serious, and it is more harmful to the human body, and it has gradually become one of the most important problems in endangering human health. At present, acute poisoning of humans and animals usually takes mercapto compounds (dimercaptopropanol (BAL), dimercaptopropanesulfonate (DMPS), etc.) for detoxification, but 50% of patients will have side effects, mainly manifested as fever , tachycardia, nausea, vomiting, night sweats, may cause the release of histamine. Lactic acid bacteria are widely used and safe microorganisms in food fermentation, and are easy to cultivate and reproduce quickly. More and more reports have found that they have good...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C12N1/04C12G3/024C02F3/34A23L7/104A21D8/04A23C9/123A23K10/12A23K50/80A23K50/75A23K50/10A23K50/30C12R1/01
CPCC12N1/36C12N1/20C12N1/04C12G3/024C02F3/34A23L7/104A21D8/045A23C9/1234A23K10/12A23K50/80A23K50/75A23K50/30A23K50/10C02F2101/20A23V2400/427
Inventor 田建军贺银凤贺敏李畅顾悦张开屏张保军张亮
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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