Method for inducing tenebrio molitor to produce antibacterial peptide, preparation method of tenebrio molitor antibacterial peptide and application of tenebrio molitor antibacterial peptide

A technology of Tenebrio molitor and antimicrobial peptide, which is applied in the field of inducing Tenebrio molitor to produce antimicrobial peptide and Tenebrio molitor antimicrobial peptide, can solve the problems of low content of antibacterial peptide, achieve the effect of increasing content, simple preparation process and low process cost

Active Publication Date: 2022-03-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tenebrio molitor antimicrobial peptide is a small molecular polypeptide produced by Tenebrio molitor under natural state or induced conditions, but the content of antimicrobial peptides in Tenebrio molitor is too low under natural state or existing induced conditions

Method used

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  • Method for inducing tenebrio molitor to produce antibacterial peptide, preparation method of tenebrio molitor antibacterial peptide and application of tenebrio molitor antibacterial peptide
  • Method for inducing tenebrio molitor to produce antibacterial peptide, preparation method of tenebrio molitor antibacterial peptide and application of tenebrio molitor antibacterial peptide
  • Method for inducing tenebrio molitor to produce antibacterial peptide, preparation method of tenebrio molitor antibacterial peptide and application of tenebrio molitor antibacterial peptide

Examples

Experimental program
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preparation example Construction

[0041] The invention provides a preparation method of Tenebrio molitor antimicrobial peptide, comprising the following steps: extracting Tenebrio molitor containing antimicrobial peptide induced in the above technical scheme through ammonium acetate extract to obtain antimicrobial peptide crude extract; The crude extract was filtered through a Sephadex column to obtain Tenebrio molitor antimicrobial peptides.

[0042] In the present invention, the Tenebrio molitor containing antimicrobial peptides is extracted with an ammonium acetate extract to obtain a crude antimicrobial peptide extract. In the present invention, preferably, the Tenebrio molitor larvae containing antimicrobial peptides are mixed with an ammonium acetate extract and then ground to obtain the ground Tenebrio molitor larvae. In the present invention, the mass volume ratio of the Tenebrio molitor containing antimicrobial peptides to the ammonium acetate extract is preferably 1-2g:2.5-3mL, more preferably 1g:3mL...

Embodiment 1

[0062] Tenebrio molitor larvae with complete body surface, pure color, no melanization, and strong mobility were placed in an incubator, without feeding for 2 consecutive days, and the culture conditions were normal temperature and dark.

[0063] Escherichia coli was cultured overnight and diluted with sterile saline to 0.5 McFarland turbidity (1~2×10 8 CFU / mL) to obtain Escherichia coli bacteria liquid, mix the above Escherichia coli bacteria liquid with wheat bran, mix 2g of wheat bran into each milliliter of bacteria liquid, and feed continuously for 24h at room temperature; Acupuncture the end of the internodes of Tenebrio molitor larvae after bacillus liquid, and acupuncture each larva twice; put the acupunctured Tenebrio molitor larvae into a disposable petri dish with a diameter of 9 cm, and irradiate vertically with a 25W ultraviolet lamp For Tenebrio molitor, the vertical distance of irradiation is 25cm, and the irradiation time is 30min; obtain Tenebrio molitor larva...

Embodiment 2

[0075] Compared with Example 1, only No. 3 sterile insect needles were used to dip in the E. coli bacterial solution and then needle the internodes of Tenebrio molitor larvae, and each larvae was needled twice, without other induction methods.

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Abstract

The invention belongs to the technical field of biological antibacterial peptides, and particularly relates to a method for inducing tenebrio molitor to produce antibacterial peptides, a preparation method of the tenebrio molitor antibacterial peptides and application of the tenebrio molitor antibacterial peptides. The invention provides a method for inducing tenebrio molitor to produce antibacterial peptide, which comprises the following steps: feeding tenebrio molitor larvae subjected to empty stomach treatment by using feed mixed with escherichia coli liquid to obtain the tenebrio molitor larvae subjected to feeding induction; needling back internodes of the tenebrio molitor larvae subjected to feeding induction to obtain the tenebrio molitor larvae subjected to needling induction; and irradiating the tenebrio molitor larvae subjected to acupuncture induction under ultraviolet light, and culturing for 24 hours to obtain the tenebrio molitor containing the antibacterial peptide. Compared with a conventional induction method, the method has the advantages that the tenebrio molitor is subjected to composite induction, the tenebrio molitor is stimulated by adopting the induction method and the induction sequence provided by the invention, and the content of the antibacterial peptide can be increased to a greater extent.

Description

technical field [0001] The invention belongs to the technical field of biological antibacterial peptides, and in particular relates to a method for inducing Tenebrio molitor to produce antimicrobial peptides, a preparation method and application of Tenebrio molitor antibacterial peptides. Background technique [0002] In recent years, with the continuous development of my country's aquaculture industry, the phenomenon of streptococcal infection in fish has also occurred continuously. The means by which farmers in my country prevent fish from being infected by pathogenic bacteria is generally to inject a large amount of antibiotics into the breeding area before the outbreak of the disease in summer every year. It has a certain effect in the early stage, but as time goes on, the bacteria are prone to drug resistance and weaken. the effect of antibiotics. In addition, in 2019, the Ministry of Rural Agriculture issued a "ban on antibiotics" to stop the production of animal feed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K10/18A23K10/20A23K20/147A23K50/90A23K50/10A23K50/30A23K50/80C07K14/435C07K1/14C07K1/16
CPCA01K67/033A23K10/18A23K10/20A23K20/147A23K50/90A23K50/10A23K50/30A23K50/80C07K14/43563
Inventor 孟赫诚蔡萍瑶林勇黎先锋刘勇
Owner SOUTH CHINA UNIV OF TECH
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