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Application of 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as plant immune resistance inducer

A technology of indolyl butyric acid and methyl pyrrolidine, applied in the field of agricultural biological pesticides, can solve problems such as biological activity blanks, and achieve the effects of improving resistance, broad application prospects and low dosage

Active Publication Date: 2022-03-04
NANJING TIANNONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, the research on 3-methylpyrrolidine-2-carboxylic acid has mostly focused on the polypeptide hydrolysis and biosynthetic pathways, and there is no report on its free existence, and the research on its biological activity has so far been limited. still blank

Method used

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  • Application of 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as plant immune resistance inducer
  • Application of 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as plant immune resistance inducer
  • Application of 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as plant immune resistance inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 (biological synthesis, extraction method and structural identification of the compound of the present invention)

[0046] (1) Cultivation of Alternaria

[0047] Glucose sodium nitrate medium: glucose, 40.0g; NaNO 3 , 1.0g; NH 4 Cl, 0.25g; KH 2 PO 4 , 1.0g; KCl, 0.25g; NaCl, 0.25g; MgSO 4 ·7H 2 O, 0.5g; FeSO 4 ·7H 2 O, 0.01g; ZnSO 4 ·7H 2 O, 0.01g; yeast extract, 1g, add water to 1L, adjust pH to 5.5.

[0048] The Alternaria culture method is as follows: PDA medium activates the preserved strains, and after 7 days, select colonies with consistent growth, take a 5mm diameter bacterial cake, and inoculate it into 500mL medium, and the inoculation amount is one bacterial cake per 100mL. Place the medium inoculated with the bacterial blocks into a constant temperature shaker, and the culture conditions are: 140 rpm, 25°C, and dark culture for 7 days.

[0049] (2) Extraction of compounds

[0050]The mycelia were separated from the fermentation broth a...

Embodiment 2

[0063] Example 2 (2-amino-3-indolyl butyric acid and 3-methylpyrrolidine-2-carboxylic acid induce tobacco resistance to tomato spotted wilt virus infection)

[0064] Tomato spotted wilt virus was obtained from Yunnan Province, China. The initial virus source was stored in a -80°C refrigerator. The virus was inoculated on the leaves of Nicotiana benthamiana by friction inoculation to activate the virus, and the virus plasmid was extracted using Escherichia coli competent cells. Transformation, smeared on resistant plates and cultured, picked a single colony for PCR screening, selected positive colonies for sequencing and subsequent plasmid extraction, added the plasmids with normal sequencing to Agrobacterium competent cells, and carried out agronomy by electroshock method. For Bacillus transformation, the transformed Agrobacterium solution was spread on the corresponding resistance screening plate, and cultured at 28°C (±1) for 48h. Pick a single colony of Agrobacterium on the...

Embodiment 3

[0083] Example 3 (2-amino-3-indole butyric acid and 3-methylpyrrolidine-2-carboxylic acid induce Arabidopsis resistance to Pseudomonas syringae infection)

[0084]Dissolve 2-amino-3-indolebutyric acid in sterile water, and then use sterile water to gradiently dilute it into 100nM, 1000nM and 10000nM solutions, and set up a blank control, and add 0.02% Tween 20 as a surfactant at the same time . Spread Pseudomonas syringae PstDC3000 on an LB plate and culture at 28°C for 48 hours; pick a single colony and inoculate it into a 50mL centrifuge tube containing 2mL of medium, culture it on a shaker at 28°C and 250rpm, monitor every 1-2h Bacteria solution OD 600 value change, at OD 600 Stop culturing bacteria before the value reaches 0.8; transfer 1mL of bacterial liquid to a sterile 1.5mL centrifuge tube, centrifuge at 8000rpm for 2min, and collect the precipitate; remove the supernatant, wash the precipitate with 10mM magnesium chloride for 3 times and centrifuge, and finally res...

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Abstract

The invention discloses an application of 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as a plant immune resistance inducer, and relates to a plant immune resistance inducer. 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid is used as a natural active substance to be developed into a plant immune resistance inducer, and the plant immune resistance inducer can be used for improving the resistance of plants to biological stress and abiotic stress and effectively preventing fungi, bacteria and viruses from infecting the plants, so that the pathogenic level is reduced; meanwhile, the tolerance of plants to high temperature, low temperature, drought and salt stress can be remarkably improved. The 2-amino-3-indolyl butyric acid or the 3-methylpyrrolidine-2-carboxylic acid has the characteristics of safety, environmental protection and high efficiency.

Description

technical field [0001] The invention belongs to the field of agricultural biological pesticides and relates to the application of 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as a plant immune inducer. Background technique [0002] In agricultural production, losses caused by abiotic stresses such as high temperature, low temperature, drought and salt are huge. In recent years, extreme weather has occurred frequently around the world, and the stress faced by agricultural plants has become increasingly serious. High temperature and low temperature seriously affect the growth and development of plants, thereby affecting the yield and quality of plants. Drought is one of the most important adversity stress factors affecting the survival, growth and distribution of plants. The current global arid and semi-arid areas account for more than 40% of the total cultivated land area. In recent years, due to the deterioration of the global climate, the cycle o...

Claims

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Application Information

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IPC IPC(8): A01N43/38A01N43/36A01P3/00A01P21/00
CPCA01N43/38A01P21/00A01G7/06Y02A40/10
Inventor 陈世国王赫王良省房婉萍郭爱平
Owner NANJING TIANNONG BIOTECHNOLOGY CO LTD
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