Industrial preparation method of L-histidine

Abstract

The invention relates to an industrial preparation method of L-histidine, which comprises the following steps: step 1, adding L-histidine hydrochloride into purified water, stirring and dissolving at room temperature, then regulating the pH value of the solution to be neutral by adopting alkali liquor, and preparing a salt solution of L-histidine for later use; step 2, performing electrodialysis treatment on the salt solution of the L-histidine by adopting an electrodialysis desalting water tank, and stopping electrodialysis until the conductivity of desalting liquid is less than or equal to 1000ms / cm to obtain an aqueous solution of the L-histidine; in the electrodialysis treatment process, the pH of the desalted liquid is controlled to be neutral; and step 3, condensing the desalted L-histidine aqueous solution at high temperature under reduced pressure until the volume is 0.25-0.3 time of the original volume, adding a composite organic solvent into the concentrated solution, and then carrying out cooling crystallization, centrifugal filtration and drying under reduced pressure to obtain the L-histidine. The method is green, environment-friendly, low in cost and suitable for commercial production.

Description

technical field [0001] The invention belongs to the field of preparation of L-histidine, and in particular relates to an industrial preparation method of L-histidine. Background technique [0002] L-Histidine (L-Histidine) is a basic amino acid containing an imidazole nucleus in its molecule, and is an essential amino acid for infants. It plays an important role in the process of human growth and tissue repair. It can be used to treat ulcers, hyperacidity, allergies, rheumatoid arthritis, anemia, etc. It can also be used for amino acid infusion and comprehensive amino acid preparations. Because L-histidine has various physiological functions, it has been widely used in many fields such as medicine, feed and food health care, and its role in medical research has been paid more and more attention. [0003] At present, the production method of L-histidine has following two kinds: 1), the L-histidine liquid extracted from pig hair is neutralized to pH partial acidity with hydro...

AI Technical Summary

Problems solved by technology

However, these methods have the following problems: 1) a large amount of waste liquid will be produced, and the waste liquid treatment is not only difficult but also costly, which increases the production cost of L-histidine; 2) the obtained L-histidine crystal form Unstable, improves the storage difficulty of product; 3) raw material all comes from pig hair and pig blood powder, and raw material receives the restriction of pig cycle, and raw material supply is unstable; Above these problems have all limited the suitability for industrialized production of L-histidine, As a result, L-histidine has become one of the bottleneck amino acids in my country's amino acid industry, which affects the process of my country's nationalization of all amino acid infusion raw materials.