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Lysobacter enzymogenes and application thereof

A technology for producing enzymatic lysobacteria and bacterial strains, applied in the fields of application, bacteria, and fungicides, can solve the problems of rapid variation of physiological races, environmental pollution caused by chemical control, and few preparations of biological agents to prevent and control diseases, etc., so as to achieve easy use , Good inhibitory effect

Active Publication Date: 2022-03-08
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) Due to the rapid variation of Physiological races of Pseudomonas spp. and vegetable clubroot, and the serious environmental pollution caused by chemical control, the damage of Pseudomonas spp. has shown an obvious trend of aggravation
[0009] (2) With the rapid variation of physiological races and serious environmental pollution caused by chemical control, the effect of crop fungal disease control is not ideal, and there are certain limitations
[0010] (3) Lysobacterium enzymogenes has been reported mostly from the aspects of antibacterial spectrum and disease prevention mechanism, but there is no report on obtaining it from the rhizosphere of fresh corn, and few of them have been prepared into biological agents for field disease control.

Method used

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  • Lysobacter enzymogenes and application thereof
  • Lysobacter enzymogenes and application thereof
  • Lysobacter enzymogenes and application thereof

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preparation example Construction

[0045] Such as figure 1 As shown, the preparation method of Lysobacter zymogenes BN3-15 aqueous solution provided by the embodiment of the present invention comprises:

[0046] S101, rejuvenation culture: sterilize the NA medium at 121°C for 30 minutes, add it to the test tube, make a slope, inoculate the strain, and incubate in a constant temperature incubator at 26°C for 24-48 hours;

[0047] S102, expanded culture of the strain on a shaker: inoculate the slant seeds on the NA medium of the eggplant vase slant, and culture at 26°C for 24-48 hours to obtain the eggplant vase seeds;

[0048] S103, preparation of the seed suspension: wash the bacterial lawn on the slope of the eggplant bottle with sterilized water to obtain the seed suspension;

[0049] S104, inoculation: the seed suspension is passed through the inoculation port, and connected to the culture medium of the fermenter by using the pressure difference;

[0050] S105, liquid fermentation: after the inoculation of...

Embodiment 1

[0056] Embodiment 1: the preparation of enzyme-producing lysobacterium (Lysobacter enzymogenes) BN3-15 water

[0057] (1) Source of strain: Lysobacter enzymogenes BN3-15 was isolated from corn rhizosphere soil in Xishuangbanna Dai Autonomous Prefecture, Yunnan Province.

[0058] (2) Bacteria culture

[0059] A. test tube seed culture (the following are weight percentages):

[0060] Put the NA medium into a test tube, make a slant, inoculate the BN3-15 strain of Lysobacter zymogenes, and incubate at 26°C for 24h. NA medium preparation and sterilization methods are as follows: peptone 5g, sucrose 10g, yeast powder 1g, beef extract 3g, agar 17-20g, make up to 1000mL with distilled water, adjust the pH to 7.0, and then sterilize at 121°C for 30min.

[0061] b. Bacteria Shaker Expansion Cultivation:

[0062] Inoculate the seeds obtained in the above steps on the eggplant bottle slant medium (the medium is NA medium, the formula of which is the same as that of the aforementioned ...

Embodiment 2

[0068] Embodiment 2: the preparation of enzyme-producing lysobacterium (Lysobacter enzymogenes) BN3-15 wettable powder

[0069] (1) prepare liquid biocontrol agent: with embodiment 1, obtain fermented liquid;

[0070] (2) Add filler: Put the fermentation hydraulic pressure into the storage tank, add diatomite (light calcium carbonate) as filler according to the following calculation formula, and stir for 30 minutes;

[0071] Adding amount of filler (kg)=[number of fermentation broth bacteria (100 million / ml)×can volume (liter)×yield] / number of finished product bacteria-residue in fermentation broth (kg). Then, it is filtered by plate and frame, beaten, dried and crushed to make wettable powder for field application.

[0072] (3) Plate and frame filtration: use 2kg / cm 2 Press the material into the plate frame from the storage tank, filter through No. 7 filter cloth, adjust the pressure at any time, so that the bacteria content in the filtrate is not more than 0.2 billion / ml, ...

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Abstract

The invention belongs to the technical field of biological prevention and control, and discloses lysobacter enzymogenes and application thereof.The lysobacter enzymogenes are lysobacter enzymogenes BN3-15 strains, the strains are separated from rhizosphere soil of fresh corn in Dai autonomous zhou of Xishuangbanna in Yunnan province, and the strains are classified and named as lysobacter enzymogenes; the lysobacter enzymogenes BN3-15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lysobacter enzymogenes BN3-15 is CGMCC No. 21259. The lysobacter enzymogenes BN3-15 provided by the invention is safe and residue-free, has the effects of preventing diseases and promoting growth, can be prepared into an aqueous solution, wettable powder or a seed coating agent, and is used for preventing and treating diseases such as northern corn leaf blight, pepper anthracnose, Chinese cabbage clubroot, panax notoginseng root rot and the like in fields. The biological agent provided by the invention is relatively good in comprehensive character, remarkable in fungal disease control effect, beneficial to reducing the use amount and residual quantity of chemical agents in agricultural products, relatively safe to use and easy for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biological control, in particular to an enzyme-producing lysobacterium and its application. Background technique [0002] At present, corn (Zea mays Linn.) is an annual herbaceous plant of the Poaceae family, which is an important grain-forage-industrial raw material crop in my country, and is also one of the crops with the most extensive planting area and the largest land utilization rate in China, and it is also the largest planting area in Yunnan Province. crop. Yunnan Province can be divided into four corn-growing regions: Northwest Yunnan cool corn region, Central Yunnan warm corn region, Southern Yunnan warm corn region, and Northeast Yunnan warm and cool corn region. In recent years, the planting area of ​​fresh corn in our province has gradually expanded, and it has become a pillar industry with plateau characteristics. The main disease types of grain corn in Yunnan Province are corn rust, corn g...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P21/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 魏兰芳姬广海代真林汪娅婷
Owner YUNNAN AGRICULTURAL UNIVERSITY
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