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Application of adenosine or adenosine monophosphate in preparation of anti-infective drugs

An adenosine monophosphate, anti-infection technology, applied in the field of biomedicine, can solve the problems of limited small molecules, unable to meet the needs of antibiotics and pathogenic bacteria, achieve good anti-infection effect, and reduce the effect of bacterial drug resistance

Pending Publication Date: 2022-03-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects and insufficiencies of existing small molecules that can improve the sensitivity of pathogenic bacteria to antibiotics, and their effects are specific to antibiotics and pathogenic bacteria, and cannot meet the needs of all antibiotics and pathogenic bacteria. Application of small molecules that increase the sensitivity of pathogenic bacteria to antibiotics in the preparation of anti-infective drugs

Method used

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  • Application of adenosine or adenosine monophosphate in preparation of anti-infective drugs
  • Application of adenosine or adenosine monophosphate in preparation of anti-infective drugs
  • Application of adenosine or adenosine monophosphate in preparation of anti-infective drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Bacterial Sample Preparation

[0035] Pick a single bacterial colony and inoculate it in 5 mL of LB medium, at 37°C (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Coccus faecium, Streptococcus iniae, Acinetobacter baumannii, Streptococcus pyogenes cocci) or 30°C (Aeromonas hydrophila, Edwardsiella tarda, Vibrio alginolyticus, Vibrio parahaemolyticus) at 200rpm for 16 hours; transfer the bacterial solution to fresh LB medium at a ratio of 1:100, at 37°C or Cultivate to OD at 30°C 200rpm 600 1.0, take an appropriate amount of bacterial liquid at 8000rpm, collect the bacterial cells by centrifugation for 5min, remove the supernatant and wash the bacterial cells with an equal volume of 0.85% normal saline for 3 times, suspend in M9 medium (containing 10mM lactose, 2mM MgSO 4 , 0.1mM CaCl 2 ), and use M9 medium to adjust the concentration of the bacteria solution to OD 600 is 0.2, and dispensed into 5mL test tubes for later use.

Embodiment 2

[0036] Example 2 Determination of the effect of adenosine and adenosine monophosphate on improving clinical Klebsiella multidrug-resistant bacteria to cefoperazone-sulbactam antibiotic susceptibility

[0037] Prepare 4 strains of bacterial samples according to Example 1, and the bacteria include 2 strains of clinical Klebsiella multidrug-resistant bacteria (No. Kpn4 and No. Kpn 9), 2 strains of Escherichia coli (1 strain of model Escherichia coli is K12, clinical multidrug-resistant Escherichia coli One strain is EC-Y17). Each bacterial strain was divided into 5 groups, which were the control group of M9 medium, cefoperazone-sulbactam (perazone-sulbactam) group, cefoperazone-sulbactam+adenosine group, cefoperazone-sulbactam+adenosine group, and cefoperazone-sulbactam+ Adenosine monophosphate group, cefoperazone-sulbactam+adenine group; the concentration of cefoperazone-sulbactam was 160ug / mL (clinical Klebsiella multidrug-resistant bacteria) or 30ug / mL (Escherichia coli), aden...

Embodiment 3

[0044] Example 3 Adenosine and adenosine monophosphate can improve the sensitivity of clinical Klebsiella multidrug-resistant bacteria to cefoperazone-sulbactam in a concentration-dependent manner

[0045] In order to study the optimal bactericidal concentration ratio between the combined use of cefoperazone sulbactam and adenosine, the combined use of cefoperazone sulbactam and adenosine monophosphate and the bactericidal efficiency, the prepared clinical Klebsiella multi-resistant Drug No. 4 strain, Kpn4, was added with different concentrations (from 20 to 320 μg / ml) of cefoperazone sulbactam and different concentrations (from 0.25 to 16 mM) of adenosine, or different concentrations of adenosine monophosphate (from 0.25 to 16 mM) ) were combined; each combination had three biological repetitions; the control group was M9 medium. Incubate in a shaker at 37°C and 200rpm for 6 hours, then take 100μL and use serial dilution method, take 10μL respectively for plate counting, and ...

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Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to application of adenosine or adenosine monophosphate in preparation of anti-infective drugs. The invention relates to a preparation method of the medicine. Adenosine or adenosine monophosphate can significantly improve the effect of clinical escherichia coli, aeromonas hydrophila, vibrio including vibrio alginolyticus and vibrio parahaemolyticus, streptococcus pyogenes, pseudomonas aeruginosa, bacillus faecium, streptococcus iniae, acinetobacter baumannii, klebsiella pneumoniae and other bacteria on the effects of cefoperazone sulbactam, ceftazidime, ceftriaxone sodium, ceftazidime, ceftriaxone sodium, ceftazidime, ceftriaxone sodium, ceftazidime sodium and the like. The sensitivity of antibiotics such as cefoperazone, meropenem, imipenem, ciprofloxacin, ampenem, moxifloxacin, levofloxacin, gentamicin, amikacin, kanamycin and the like is improved, the compound can be used together with the antibiotics to serve as an anti-infection drug, bacteria are killed under the condition of low-concentration antibiotics, and a good anti-infection effect is achieved; meanwhile, the generation of bacterial drug resistance is reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of adenosine or adenosine monophosphate in the preparation of anti-infective drugs. Background technique [0002] Antibiotics can effectively inhibit or kill pathogenic bacteria and play a key role in the control of bacterial infections. However, with the widespread use of antibiotics, many pathogenic bacteria have begun to develop resistance to antibiotics, making the infections caused by them difficult to control and seriously endangering people's health. The World Health Organization (WHO) clearly pointed out in the 2007 "World Health Report" that bacterial drug resistance is a major public health problem that threatens human health. [0003] At present, in order to reduce drug-resistant bacteria and treat infections caused by drug-resistant bacteria, in addition to reducing and limiting the use of antibiotics, improving the sensitivity of...

Claims

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Application Information

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IPC IPC(8): A61K31/7076A61K45/06A61P31/04
CPCA61K31/7076A61K45/06A61P31/04A61K2300/00Y02A50/30
Inventor 李惠彭博彭宣宪项娟娟陶建军
Owner SUN YAT SEN UNIV
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