Application of steroid hormone and metabolite thereof
A technology of steroid hormones and regulators, applied in the application field of steroid hormones and their metabolites
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Embodiment 1 Embodiment 1
[0071] Example 1 Example 1: Identification of algal steroid hormone metabolism and regulation key enzymes or proteins
[0072] Step 1. Algal species selection
[0073] The algal species involved in the present invention include (1) green algae, such as Micromonas sp.RCC299, Ostreococcustauri, Chlorella variabilis NC64A, Haematococcus pluvialis, Chlorellapyrenoidosa, Chlorella subellipsoidea C-169, Chlamydomonas reinhardtii, Volvoxcarteri etc.; (2) red algae, such as Cyanidioschyzon merolae,Porphyridium purpureum,Gelidiumamansii,Gloiopeltisfurcata,Caloglossa leprieurii,Digenea simples等;(3)硅藻,如Phaeodactylum tricornutum,Thalassiosirapseudonana,Fragilariopsis cylindrus等;(4)褐藻,如Pelvetia canaliculata,Ecklonia hornem,Undariapinnatifida,Ectocarpussiliculosus等;( 5) Euopsis algae, such as Nannochloropsis oceanica, etc., but not limited to the above-mentioned algae species.
[0074] Step 2. Data Collection
[0075] The data sources involved in the present invention include The Conserve...
Embodiment 2
[0079] Example 2: Identification of key enzymes or proteins involved in the synthesis, signal transmission and regulation of steroid hormones in the process of stress resistance and oil accumulation in microalgae
[0080] Step 1. Algal seed cultivation and sample collection
[0081] Take Nannochloropsis oceanica as an example. Using optimized F / 2 seawater culture medium, the formula is as follows: 35g l -1 sea salt, 1g l -1 NaNO 3 , 67 mg l -1 NaH 2 PO 4 *H 2 O, 3.65 mg l -1 FeCl 3 *6H 2 O,4.37mg l -1 Na 2 EDTA*2H 2 O, trace metal mix (0.0196mg l -1 CuSO 4 *5H 2 O, 0.0126 mg l -1 NaMoO 4 *2H 2 O,0.044mg l -1 ZnSO 4 *7H 2 O,0.01mg l -1 CoCl 2 , and 0.36mg l-1MnCl 2 *4H 2 O), and vitamin mix (2.5 μg l-1VB12, 2.5 μg l-1biotin, and 0.5 μg l-1thiamine HCl). Cells at 50 μmolphotons m -2 the s -1 Incubate at 25°C under continuous light. Cultured to logarithmic phase (OD 750 =3.0) Nannochloropsis centrifuge collected, washed 3 times with sterilized seaw...
Embodiment 3
[0092] Embodiment 3: the application of steroid hormone in promoting the growth of algae
[0093] Step 1. Algae cultivation and sample collection Take N. oceanica as an example. Using optimized F / 2 seawater culture medium, the formula is as follows: 35g L -1 sea salt, 1g L -1 Sodium nitrate (NaNO 3 ), 67 mg L -1 Sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 *H 2 O), 3.65 mg L -1 Ferric chloride hexahydrate (FeCl 3 *6H 2 O), 4.37 mg L -1 Sodium citrate dihydrate (Na 2 EDTA*2H 2 O), trace elements [0.0196mg L -1 Copper sulfate pentahydrate (CuSO 4 *5H 2 O), 0.0126 mg L -1 Sodium molybdate dihydrate (NaMoO 4 *2H 2 O), 0.044 mg L -1 Zinc sulfate heptahydrate (ZnSO 4 *7H 2 O), 0.01 mg L -1 Cobalt Chloride (CoCl 2 ) and 0.36mg L -1 Manganese chloride tetrahydrate (MnCl 2 *4H 2 O)] and vitamin mixture (2.5 μg L -1 Vitamin B12, 2.5 μg L -1 biotin and 0.5 μg L -1 Thiamine hydrochloride). cells in 50 μmol photons m -2 the s -1 Under continuous ligh...
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