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Application of steroid hormone and metabolite thereof

A technology of steroid hormones and regulators, applied in the application field of steroid hormones and their metabolites

Pending Publication Date: 2022-03-11
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no research on the correlation between steroid hormones and algae life activities. What are the similarities and differences between steroid hormone metabolic pathways and higher plants, whether they can be used in industrial production of algae and how to apply them are still a mystery for most researchers. issues that need resolving

Method used

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  • Application of steroid hormone and metabolite thereof
  • Application of steroid hormone and metabolite thereof
  • Application of steroid hormone and metabolite thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 Embodiment 1

[0071] Example 1 Example 1: Identification of algal steroid hormone metabolism and regulation key enzymes or proteins

[0072] Step 1. Algal species selection

[0073] The algal species involved in the present invention include (1) green algae, such as Micromonas sp.RCC299, Ostreococcustauri, Chlorella variabilis NC64A, Haematococcus pluvialis, Chlorellapyrenoidosa, Chlorella subellipsoidea C-169, Chlamydomonas reinhardtii, Volvoxcarteri etc.; (2) red algae, such as Cyanidioschyzon merolae,Porphyridium purpureum,Gelidiumamansii,Gloiopeltisfurcata,Caloglossa leprieurii,Digenea simples等;(3)硅藻,如Phaeodactylum tricornutum,Thalassiosirapseudonana,Fragilariopsis cylindrus等;(4)褐藻,如Pelvetia canaliculata,Ecklonia hornem,Undariapinnatifida,Ectocarpussiliculosus等;( 5) Euopsis algae, such as Nannochloropsis oceanica, etc., but not limited to the above-mentioned algae species.

[0074] Step 2. Data Collection

[0075] The data sources involved in the present invention include The Conserve...

Embodiment 2

[0079] Example 2: Identification of key enzymes or proteins involved in the synthesis, signal transmission and regulation of steroid hormones in the process of stress resistance and oil accumulation in microalgae

[0080] Step 1. Algal seed cultivation and sample collection

[0081] Take Nannochloropsis oceanica as an example. Using optimized F / 2 seawater culture medium, the formula is as follows: 35g l -1 sea ​​salt, 1g l -1 NaNO 3 , 67 mg l -1 NaH 2 PO 4 *H 2 O, 3.65 mg l -1 FeCl 3 *6H 2 O,4.37mg l -1 Na 2 EDTA*2H 2 O, trace metal mix (0.0196mg l -1 CuSO 4 *5H 2 O, 0.0126 mg l -1 NaMoO 4 *2H 2 O,0.044mg l -1 ZnSO 4 *7H 2 O,0.01mg l -1 CoCl 2 , and 0.36mg l-1MnCl 2 *4H 2 O), and vitamin mix (2.5 μg l-1VB12, 2.5 μg l-1biotin, and 0.5 μg l-1thiamine HCl). Cells at 50 μmolphotons m -2 the s -1 Incubate at 25°C under continuous light. Cultured to logarithmic phase (OD 750 =3.0) Nannochloropsis centrifuge collected, washed 3 times with sterilized seaw...

Embodiment 3

[0092] Embodiment 3: the application of steroid hormone in promoting the growth of algae

[0093] Step 1. Algae cultivation and sample collection Take N. oceanica as an example. Using optimized F / 2 seawater culture medium, the formula is as follows: 35g L -1 sea ​​salt, 1g L -1 Sodium nitrate (NaNO 3 ), 67 mg L -1 Sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 *H 2 O), 3.65 mg L -1 Ferric chloride hexahydrate (FeCl 3 *6H 2 O), 4.37 mg L -1 Sodium citrate dihydrate (Na 2 EDTA*2H 2 O), trace elements [0.0196mg L -1 Copper sulfate pentahydrate (CuSO 4 *5H 2 O), 0.0126 mg L -1 Sodium molybdate dihydrate (NaMoO 4 *2H 2 O), 0.044 mg L -1 Zinc sulfate heptahydrate (ZnSO 4 *7H 2 O), 0.01 mg L -1 Cobalt Chloride (CoCl 2 ) and 0.36mg L -1 Manganese chloride tetrahydrate (MnCl 2 *4H 2 O)] and vitamin mixture (2.5 μg L -1 Vitamin B12, 2.5 μg L -1 biotin and 0.5 μg L -1 Thiamine hydrochloride). cells in 50 μmol photons m -2 the s -1 Under continuous ligh...

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Abstract

The invention relates to the technical field of microalgae, in particular to application of a steroid hormone and a regulator thereof. The research finds that the physiological metabolism process of the algae can be regulated by exogenously giving the steroid hormone or utilizing the steroid hormone regulator, including regulating or controlling the growth of the algae, the accumulation of secondary metabolites of the algae or the stress resistance of the algae and the like. Experiments show that by exogenously giving the steroid hormone and overexpressing key enzyme genes (such as steroid hormone synthetic genes) for improving the steroid hormone level, the growth of algae can be promoted.

Description

technical field [0001] The invention relates to the technical field of microalgae, in particular to the application of steroid hormones and their metabolites. Background technique [0002] Microalgae absorb solar energy, capture carbon dioxide, and synthesize various high-value chemicals, which have broad application prospects in the fields of environment, agriculture, and aquaculture. Marine microalgae can be cultivated in coastal tidal flats and saline-alkali land, without using fresh water or competing with food for land, and have the possibility of industrialization. However, its economic feasibility still faces great challenges. The main technical bottlenecks include low growth density of microalgae, poor stress resistance and low oil content. The existing methods for regulating the growth and stress resistance of microalgae are complex and costly. Therefore, it is urgent to develop a novel method for regulating the growth of microalgae. [0003] Steroid hormones, al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12N1/13C12N15/82C12R1/89
CPCC12N1/38C12N1/12C12N15/8261C12N15/8271C12N15/8247C12N15/8291C12N15/8269
Inventor 路延笃邓湘子周文序
Owner HAINAN UNIVERSITY
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