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Immobilized microbial agent for offshore oil pollution remediation

A technology for pollution remediation and offshore oil, applied in immobilized enzymes, methods based on microorganisms, immobilized on/in organic carriers, etc., can solve problems such as lack of mechanical strength, limited activity, poor stability, etc., to achieve Improve mass transfer performance, improve mechanical strength, and improve the effect of survival rate

Pending Publication Date: 2022-03-15
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation methods of immobilized microorganisms mainly include adsorption method, embedding method, enveloping method, covalent method and cross-linking method, etc. The immobilization method of adsorption method is simple, the conditions are mild, and the carrier can be regenerated; The binding force is weak, and the microorganisms are easy to fall off, so the stability in the application is not good
The cross-linking method has the advantage of being able to obtain very high cell densities, but it cannot be widely used due to the lack of good mechanical strength
The covalent immobilized microorganism has good stability and is not easy to fall off, but its activity is limited, and the reaction is intense, and the operation and control are complicated and harsh.

Method used

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  • Immobilized microbial agent for offshore oil pollution remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of Immobilized Bacteria

[0041] 1. Modification of sodium alginate

[0042] Take 1g of sodium alginate and add 20g of water, stir until completely dissolved and then heat to 60°C; add 0.1g of ammonium persulfate under nitrogen protection, stir for 10 minutes and then cool down to 0°C; slowly add 0.8g of allylamine hydrochloride, and heat up after adding React at 70°C for 2.5 hours; after the reaction, adjust the pH to neutral, dialyze with water for 48 hours, and freeze-dry to obtain modified sodium alginate.

[0043] 2. Preparation of bacterial suspension

[0044] Separately preserved in glycerol Marinobacter aromaticivorans (Accession No. CGMCC111015) and Hyphomonas adhaerens (Accession No. ATCC43965) After being activated in the medium, expand the culture at a constant temperature of 25°C to OD630=1.2, and then dilute to 10 9 CFU / mL, take 90g Marinobacter aromaticivorans Bacterial suspension and 10g Hyphomonas adhaerens The bacterial suspension...

Embodiment 2

[0048] Preparation of Immobilized Bacteria

[0049] The difference between this embodiment and embodiment 1 is:

[0050] 3. Preparation of immobilized bacterial agent

[0051] Take 40g of PVA, 20g of modified sodium alginate, 7.5g of bentonite, 3.2g of methyl indole-3-carboxylate, and 1000g of seawater for high-temperature sterilization, cool to 80°C after sterilization, mix PVA, modified sodium alginate, and bentonite and seawater; cool down to 40°C again, add 100g of bacterial suspension and mix well; after mixing, use a peristaltic pump to add the mixed solution dropwise into the saturated boric acid mixture containing 3% calcium chloride, keep stirring for 24 hours, and then filter out the microspheres. Immobilized bacterial agent can be obtained after washing.

Embodiment 3

[0053] Preparation of Immobilized Bacteria

[0054] The difference between this example and Example 1 is that no bentonite is added.

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Abstract

The invention discloses an immobilized microbial agent for offshore oil pollution remediation, and belongs to the technical field of bioremediation. The immobilized microbial agent for offshore oil pollution remediation comprises PVA (polyvinyl alcohol), modified sodium alginate and bentonite, wherein the modified sodium alginate is modified by allylamine hydrochloride. The modification method of the modified sodium alginate comprises the following steps: adding water into sodium alginate, stirring until the sodium alginate is completely dissolved, and heating; adding ammonium persulfate under the protection of nitrogen, and cooling after stirring; adding allylamine hydrochloride, and then raising the temperature to react; and after the reaction is finished, adjusting the pH value, dialyzing with water, and freeze-drying to obtain the modified sodium alginate. The modified sodium alginate can overcome the problem of low mechanical strength of sodium alginate, so that the mechanical strength of the immobilized microbial agent is increased, and the durability is improved. And the prepared immobilized microbial agent has good mass transfer capability and strong crude oil degradation capability.

Description

technical field [0001] The invention relates to the technical field of bioremediation, in particular to an immobilized bacterial agent for repairing marine oil pollution. Background technique [0002] Due to the uneven distribution and consumption of oil around the world and the low cost of sea transportation, oil transportation between countries is mainly based on ocean transportation. With the continuous improvement of exploration and mining technology, the oil resources in the ocean are valued by countries all over the world. Marine oil spills occur frequently due to human factors and natural disasters. When these accidents happened, tens of thousands of tons of crude oil entered the marine environment, causing huge ecological damage. [0003] Petroleum is an extremely complex mixture in terms of composition and phase state, among which hydrocarbons are mainly alkanes, cycloalkanes and aromatic hydrocarbons, in addition to various organic compounds containing nitrogen, ...

Claims

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Application Information

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IPC IPC(8): C12N11/084C12N11/10C12N1/20C02F3/34C12R1/01C12R1/32
CPCC12N11/084C12N11/10C12N1/20C02F3/344Y02A20/204
Inventor 李振华周永东朱文斌卢占晖
Owner MARINE FISHERIES RES INST OF ZHEJIANG